(Linear) (Six-base) MAPSORT of: KIEE3477.seq Check: 791 from: 1 to: 4812 KIEE3477.orf With 182 enzymes: SgfI * July 3, 2012 17:57 .. Acc65I G'GTAC_C Cuts at: 0 3936 4812 Size: 3936 876 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 1427 2575 4812 Size: 103 1324 1148 2237 Fragments arranged by size: 2237 1324 1148 103 AfeI AGC'GCT Cuts at: 0 4098 4812 Size: 4098 714 AflII C'TTAA_G Cuts at: 0 3844 4812 Size: 3844 968 AflIII A'CryG_T Cuts at: 0 2683 4812 Size: 2683 2129 AhdI GACnn_n'nnGTC Cuts at: 0 4779 4812 Size: 4779 33 AleI CACnn'nnGTG Cuts at: 0 3136 4812 Size: 3136 1676 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1482 4563 4812 Size: 1482 3081 249 Fragments arranged by size: 3081 1482 249 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3251 3283 4143 4175 4812 Size: 3251 32 860 32 637 Fragments arranged by size: 3251 860 637 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 1420 1693 2271 3533 4634 4812 Size: 1420 273 578 1262 1101 178 Fragments arranged by size: 1420 1262 1101 578 273 178 ApoI r'AATT_y Cuts at: 0 808 1378 1949 2987 3898 4559 4812 Size: 808 570 571 1038 911 661 253 Fragments arranged by size: 1038 911 808 661 571 570 253 AvaI C'yCGr_G Cuts at: 0 3905 4812 Size: 3905 907 BamHI G'GATC_C Cuts at: 0 3597 4812 Size: 3597 1215 BanI G'GyrC_C Cuts at: 0 4 3506 3936 4812 Size: 4 3502 430 876 Fragments arranged by size: 3502 876 430 4 BanII G_rGCy'C Cuts at: 0 3394 3695 3995 4004 4812 Size: 3394 301 300 9 808 Fragments arranged by size: 3394 808 301 300 9 BbsI GAAGACnn'nnnn_ Cuts at: 0 1016 2500 3560 4487 4778 4812 Size: 1016 1484 1060 927 291 34 Fragments arranged by size: 1484 1060 1016 927 291 34 BbvCI CC'TCA_GC Cuts at: 0 31 49 182 4812 Size: 31 18 133 4630 Fragments arranged by size: 4630 133 31 18 BciVI GTATCCnnnnn_n' Cuts at: 0 3387 4812 Size: 3387 1425 BglII A'GATC_T Cuts at: 0 1019 2517 2662 4812 Size: 1019 1498 145 2150 Fragments arranged by size: 2150 1498 1019 145 BlpI GC'TnA_GC Cuts at: 0 3789 4812 Size: 3789 1023 Bme1580I G_kGCm'C Cuts at: 0 3511 4812 Size: 3511 1301 BmgBI CAC'GTC Cuts at: 0 4065 4812 Size: 4065 747 BmrI ACTGGGnnnn_n' Cuts at: 0 2589 4812 Size: 2589 2223 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2408 4812 Size: 2408 2404 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 515 578 837 1044 3834 4149 4812 Size: 515 63 259 207 2790 315 663 Fragments arranged by size: 2790 663 515 315 259 207 63 Bpu10I CC'TnA_GC Cuts at: 0 31 49 182 4072 4812 Size: 31 18 133 3890 740 Fragments arranged by size: 3890 740 133 31 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 616 1577 2223 2651 4353 4812 Size: 616 961 646 428 1702 459 Fragments arranged by size: 1702 961 646 616 459 428 BsaI GGTCTCn'nnnn_ Cuts at: 0 3938 4812 Size: 3938 874 BsaAI yAC'GTr Cuts at: 0 363 1359 4812 Size: 363 996 3453 Fragments arranged by size: 3453 996 363 BsaBI GATnn'nnATC Cuts at: 0 725 2048 2522 3596 4812 Size: 725 1323 474 1074 1216 Fragments arranged by size: 1323 1216 1074 725 474 BsaHI Gr'CG_yC Cuts at: 0 4328 4812 Size: 4328 484 BsaWI w'CCGG_w Cuts at: 0 1110 4812 Size: 1110 3702 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 239 269 4812 Size: 239 30 4543 Fragments arranged by size: 4543 239 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 55 66 2146 3033 4224 4370 4812 Size: 55 11 2080 887 1191 146 442 Fragments arranged by size: 2080 1191 887 442 146 55 11 BseYI C'CCAG_C Cuts at: 0 1515 3292 4051 4303 4350 4812 Size: 1515 1777 759 252 47 462 Fragments arranged by size: 1777 1515 759 462 252 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 2392 3499 4812 Size: 208 2184 1107 1313 Fragments arranged by size: 2184 1313 1107 208 BsiHKAI G_wGCw'C Cuts at: 0 1707 3995 4070 4812 Size: 1707 2288 75 742 Fragments arranged by size: 2288 1707 742 75 BsmI GAATG_Cn' Cuts at: 0 359 975 1890 1937 3128 3311 4812 Size: 359 616 915 47 1191 183 1501 Fragments arranged by size: 1501 1191 915 616 359 183 47 Bsp1286I G_dGCh'C Cuts at: 0 1707 3394 3511 3695 3995 4004 4070 4812 Size: 1707 1687 117 184 300 9 66 742 Fragments arranged by size: 1707 1687 742 300 184 117 66 9 BspEI T'CCGG_A Cuts at: 0 1110 4812 Size: 1110 3702 BspHI T'CATG_A Cuts at: 0 2231 2511 4812 Size: 2231 280 2301 Fragments arranged by size: 2301 2231 280 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1148 1314 4812 Size: 1148 166 3498 Fragments arranged by size: 3498 1148 166 BsrDI GCAATG_nn' Cuts at: 0 3227 4446 4458 4812 Size: 3227 1219 12 354 Fragments arranged by size: 3227 1219 354 12 BstAPI GCAn_nnn'nTGC Cuts at: 0 2440 4812 Size: 2440 2372 BstXI CCAn_nnnn'nTGG Cuts at: 0 990 1251 3702 4011 4812 Size: 990 261 2451 309 801 Fragments arranged by size: 2451 990 801 309 261 BstYI r'GATC_y Cuts at: 0 939 1019 2517 2662 3597 3807 4812 Size: 939 80 1498 145 935 210 1005 Fragments arranged by size: 1498 1005 939 935 210 145 80 Bsu36I CC'TnA_GG Cuts at: 0 2129 2933 3404 4382 4812 Size: 2129 804 471 978 430 Fragments arranged by size: 2129 978 804 471 430 BtgI C'CryG_G Cuts at: 0 2649 3511 4195 4812 Size: 2649 862 684 617 Fragments arranged by size: 2649 862 684 617 BtsI GCAGTG_nn' Cuts at: 0 132 1210 3783 4812 Size: 132 1078 2573 1029 Fragments arranged by size: 2573 1078 1029 132 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2618 2653 2667 2702 4184 4219 4812 Size: 2618 35 14 35 1482 35 593 Fragments arranged by size: 2618 1482 593 35 35 35 14 DraI TTT'AAA Cuts at: 0 1895 2846 4812 Size: 1895 951 1966 Fragments arranged by size: 1966 1895 951 DraIII CAC_nnn'GTG Cuts at: 0 366 4812 Size: 366 4446 DrdI GACnn_nn'nnGTC Cuts at: 0 1842 4812 Size: 1842 2970 EaeI y'GGCC_r Cuts at: 0 4403 4812 Size: 4403 409 EarI CTCTTCn'nnn_ Cuts at: 0 272 1367 1662 1813 2039 3051 3203 3731 Size: 272 1095 295 151 226 1012 152 528 Cuts at: 3731 4052 4812 Size: 321 760 Fragments arranged by size: 1095 1012 760 528 321 295 272 226 152 151 EcoICRI GAG'CTC Cuts at: 0 3993 4812 Size: 3993 819 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 515 578 837 1044 1427 2575 3834 Size: 103 412 63 259 207 383 1148 1259 Cuts at: 3834 4149 4812 Size: 315 663 Fragments arranged by size: 1259 1148 663 412 383 315 259 207 103 63 EcoNI CCTnn'n_nnAGG Cuts at: 0 780 1069 4812 Size: 780 289 3743 Fragments arranged by size: 3743 780 289 EcoO109I rG'GnC_Cy Cuts at: 0 101 787 3090 4812 Size: 101 686 2303 1722 Fragments arranged by size: 2303 1722 686 101 EcoRI G'AATT_C Cuts at: 0 4559 4812 Size: 4559 253 EcoRV GAT'ATC Cuts at: 0 758 4812 Size: 758 4054 FspI TGC'GCA Cuts at: 0 1978 4040 4812 Size: 1978 2062 772 Fragments arranged by size: 2062 1978 772 HaeII r_GCGC'y Cuts at: 0 49 4100 4812 Size: 49 4051 712 Fragments arranged by size: 4051 712 49 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 239 271 981 1013 1252 1284 1736 1768 Size: 239 32 710 32 239 32 452 32 Cuts at: 1768 2122 2154 3028 3060 3478 3510 3979 4011 Size: 354 32 874 32 418 32 469 32 Cuts at: 4011 4654 4686 4765 4797 4812 Size: 643 32 79 32 15 Fragments arranged by size: 874 710 643 469 452 418 354 239 239 79 32 32 32 32 32 32 32 32 32 32 15 HincII GTy'rAC Cuts at: 0 834 4812 Size: 834 3978 HindIII A'AGCT_T Cuts at: 0 2416 4812 Size: 2416 2396 KpnI G_GTAC'C Cuts at: 0 3940 4812 Size: 3940 872 MfeI C'AATT_G Cuts at: 0 2630 4812 Size: 2630 2182 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 225 2939 3049 4812 Size: 225 2714 110 1763 Fragments arranged by size: 2714 1763 225 110 MscI TGG'CCA Cuts at: 0 4405 4812 Size: 4405 407 MslI CAynn'nnrTG Cuts at: 0 988 1198 1718 1855 2303 2332 2696 3136 Size: 988 210 520 137 448 29 364 440 Cuts at: 3136 3659 4652 4812 Size: 523 993 160 Fragments arranged by size: 993 988 523 520 448 440 364 210 160 137 29 MspA1I CmG'CkG Cuts at: 0 1430 1656 2856 3710 4157 4634 4812 Size: 1430 226 1200 854 447 477 178 Fragments arranged by size: 1430 1200 854 477 447 226 178 NcoI C'CATG_G Cuts at: 0 2649 3511 4812 Size: 2649 862 1301 Fragments arranged by size: 2649 1301 862 NdeI CA'TA_TG Cuts at: 0 935 2345 2544 4812 Size: 935 1410 199 2268 Fragments arranged by size: 2268 1410 935 199 NspI r_CATG'y Cuts at: 0 2228 2687 3192 4348 4436 4812 Size: 2228 459 505 1156 88 376 Fragments arranged by size: 2228 1156 505 459 376 88 PasI CC'CwG_GG Cuts at: 0 3981 4812 Size: 3981 831 PciI A'CATG_T Cuts at: 0 2683 4812 Size: 2683 2129 PflMI CCAn_nnn'nTGG Cuts at: 0 366 552 1205 1317 4812 Size: 366 186 653 112 3495 Fragments arranged by size: 3495 653 366 186 112 PfoI T'CCnGG_A Cuts at: 0 143 628 2075 4812 Size: 143 485 1447 2737 Fragments arranged by size: 2737 1447 485 143 PmlI CAC'GTG Cuts at: 0 363 4812 Size: 363 4449 PpuMI rG'GwC_Cy Cuts at: 0 101 787 3090 4812 Size: 101 686 2303 1722 Fragments arranged by size: 2303 1722 686 101 PshAI GACnn'nnGTC Cuts at: 0 3710 4812 Size: 3710 1102 PsiI TTA'TAA Cuts at: 0 4529 4812 Size: 4529 283 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2087 2119 4812 Size: 2087 32 2693 Fragments arranged by size: 2693 2087 32 PstI C_TGCA'G Cuts at: 0 1308 2595 4812 Size: 1308 1287 2217 Fragments arranged by size: 2217 1308 1287 PvuII CAG'CTG Cuts at: 0 1656 2856 3710 4157 4634 4812 Size: 1656 1200 854 447 477 178 Fragments arranged by size: 1656 1200 854 477 447 178 SacI G_AGCT'C Cuts at: 0 3995 4812 Size: 3995 817 SanDI GG'GwC_CC Cuts at: 0 787 4812 Size: 787 4025 SapI GCTCTTCn'nnn_ Cuts at: 0 3731 4812 Size: 3731 1081 ScaI AGT'ACT Cuts at: 0 4798 4812 Size: 4798 14 SexAI A'CCwGG_T Cuts at: 0 2090 4812 Size: 2090 2722 SfcI C'TryA_G Cuts at: 0 1304 1367 2591 4446 4812 Size: 1304 63 1224 1855 366 Fragments arranged by size: 1855 1304 1224 366 63 SmlI C'TyrA_G Cuts at: 0 595 1592 2238 2666 3844 4332 4812 Size: 595 997 646 428 1178 488 480 Fragments arranged by size: 1178 997 646 595 488 480 428 SspI AAT'ATT Cuts at: 0 1533 2065 3107 4812 Size: 1533 532 1042 1705 Fragments arranged by size: 1705 1533 1042 532 StuI AGG'CCT Cuts at: 0 2599 4812 Size: 2599 2213 StyI C'CwwG_G Cuts at: 0 8 2649 3511 4812 Size: 8 2641 862 1301 Fragments arranged by size: 2641 1301 862 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1845 3840 4555 4812 Size: 1845 1995 715 257 Fragments arranged by size: 1995 1845 715 257 TatI w'GTAC_w Cuts at: 0 2834 2904 3325 3758 4796 4812 Size: 2834 70 421 433 1038 16 Fragments arranged by size: 2834 1038 433 421 70 16 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3483 3515 4812 Size: 3483 32 1297 Fragments arranged by size: 3483 1297 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 367 553 1694 4607 4812 Size: 367 186 1141 2913 205 Fragments arranged by size: 2913 1141 367 205 186 XmnI GAAnn'nnTTC Cuts at: 0 400 1790 3160 4812 Size: 400 1390 1370 1652 Fragments arranged by size: 1652 1390 1370 400 Enzymes that do cut: Acc65I AcuI AfeI AflII AflIII AhdI AleI AlfI AloI AlwNI ApoI AvaI BamHI BanI BanII BbsI BbvCI BciVI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BstAPI BstXI BstYI Bsu36I BtgI BtsI CspCI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I NcoI NdeI NspI PasI PciI PflMI PfoI PmlI PpuMI PshAI PsiI PsrI PstI PvuII SacI SanDI SapI ScaI SexAI SfcI SmlI SspI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AgeI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BcgI BclI BfrBI BglI BmtI BsiEI BsiWI BsmBI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI EagI EciI FalI FseI FspAI HpaI KasI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PmeI PpiI PspOMI PspXI PvuI RsrII SacII SalI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XhoI XmaI ZraI