################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HpaI HphI KasI KpnI Ksp632I KspI MaeII MaeIII MamI MfeI MluI MroI MseI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp45I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 213 CGCG CG^CG AfaI 150 GTAC GT^AC Alw44I 301 GTGCAC G^TGCAC AlwI 195 GATCC GGATC(4/5) AocI 117 CCTGAGG CC^T[ACGT]AGG Aor51HI 162 AGCGCT AGC^GCT ApaLI 301 GTGCAC G^TGCAC AspI 634 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 428 TGGCCA TGG^CCA BlpI 339 GCTGAGC GC^T[ACGT]AGC Bpu1102I 339 GCTGAGC GC^T[ACGT]AGC BsaI 674 GGTCTC GGTCTC(1/5) BseRI 592 GAGGAG GAGGAG(10/8) BsiEI 78 CGACCG CG[GA][CT]^CG BsrBI 556 GAGCGG CCGCTC(-3/-3) BsrFI 22 GCCGGC [GA]^CCGG[CT] BstUI 213 CGCG CG^CG BstYI 194 AGATCC [GA]^GATC[CT] Bsu36I 117 CCTGAGG CC^T[ACGT]AGG CelII 339 GCTGAGC GC^T[ACGT]AGC Cfr10I 22 GCCGGC [GA]^CCGG[CT] DpnII 195 GATC ^GATC Eco47III 162 AGCGCT AGC^GCT Eco81I 117 CCTGAGG CC^T[ACGT]AGG EcoNI 588 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 54 CCAAGG C^C[AT][AT]GG HaeII 162 AGCGCT [GA]GCGC^[CT] HgaI 211 GACGC GACGC(5/10) HindIII 582 AAGCTT A^AGCTT MboI 195 GATC ^GATC MflI 194 AGATCC [GA]^GATC[CT] MluNI 428 TGGCCA TGG^CCA MscI 428 TGGCCA TGG^CCA MvnI 213 CGCG CG^CG NaeI 22 GCCGGC GCC^GGC NdeII 195 GATC ^GATC NgoMIV 22 GCCGGC G^CCGGC PaeR7I 613 CTCGAG C^TCGAG PflFI 634 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 526 CTGCAG CTGCA^G PvuII 257 CAGCTG CAG^CTG RsaI 150 GTAC GT^AC SacI 342 GAGCTC GAGCT^C Sau3AI 195 GATC ^GATC SfcI 526 CTGCAG C^T[GA][CT]AG SmlI 613 CTCGAG C^T[CT][GA]AG StyI 54 CCAAGG C^C[AT][AT]GG TspRI 432 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 634 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 613 CTCGAG C^TCGAG XhoII 194 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AlwNI 176 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 529 CAGCCGCTG ApaI 413 GGGCCC GGGCC^C 476 GGGCCC AvaI 499 CCCGAG C^[CT]CG[GA]G 613 CTCGAG BanI 25 GGCACC G^G[CT][GA]CC 574 GGCACC BbsI 655 GAAGAC GAAGAC(2/6) 640 GTCTTC BcnI 81 CCGGG CC^[GC]GG 318 CCGGG BfaI 400 CTAG C^TAG 631 CTAG BpuAI 655 GAAGAC GAAGAC(2/6) 640 GTCTTC BsgI 248 CTGCAC GTGCAG(16/14) 381 CTGCAC BsmFI 241 GGGAC GGGAC(10/14) 321 GGGAC BsoBI 499 CCCGAG C^[CT]CG[GA]G 613 CTCGAG BspMI 137 ACCTGC ACCTGC(4/8) 659 ACCTGC BstF5I 105 CATCC GGATG(2/0) 84 GGATG DsaI 144 CCGTGG C^C[GA][CT]GG 488 CCGTGG EaeI 63 CGGCCA [CT]^GGCC[GA] 428 TGGCCA FokI 105 CATCC GGATG(9/13) 84 GGATG HinfI 388 GACTC G^A[ACGT]TC 634 GACTC MaeI 400 CTAG C^TAG 631 CTAG MboII 655 GAAGA GAAGA(8/7) 641 TCTTC MspA1I 257 CAGCTG C[AC]G^C[GT]G 532 CCGCTG NciI 81 CCGGG CC^[GC]GG 318 CCGGG NlaIII 12 CATG CATG^ 33 CATG PleI 388 GACTC GAGTC(4/5) 634 GACTC PpuMI 241 GGGACCC [GA]G^G[AT]CC[CT] 321 GGGACCC StuI 92 AGGCCT AGG^CCT 230 AGGCCT TaqI 77 TCGA T^CGA 614 TCGA Tsp509I 49 AATT ^AATT 112 AATT TthHB8I 77 TCGA T^CGA 614 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AluI AspHI AvaII BanII BbvI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MslI MspI MvaI MwoI NlaIV Sau96I ScrFI TseI