(Circular) (Six-base) MAPSORT of: pFN21AE5286.seq Check: 4782 from: 1 to: 5401 pFN21AE5286 5401 bp With 182 enzymes: SgfI * January 30, 2014 17:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3211 278 Size: 53 83 186 936 1675 2468 Fragments arranged by size: 2468 1675 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2744 1937 Size: 807 4594 Acc65I G'GTAC_C Cuts at: 2722 2722 Size: 5401 AclI AA'CG_TT Cuts at: 1563 3528 3901 1563 Size: 1965 373 3063 Fragments arranged by size: 3063 1965 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3464 4848 5116 1632 Size: 1832 1384 268 1917 Fragments arranged by size: 1917 1832 1384 268 AfeI AGC'GCT Cuts at: 2169 2169 Size: 5401 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5204 AflIII A'CryG_T Cuts at: 1203 4300 5277 1203 Size: 3097 977 1327 Fragments arranged by size: 3097 1327 977 AgeI A'CCGG_T Cuts at: 4232 4232 Size: 5401 AhdI GACnn_n'nnGTC Cuts at: 4130 4130 Size: 5401 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5401 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2054 2331 1184 Size: 870 277 4254 Fragments arranged by size: 4254 870 277 AlwNI CAG_nnn'CTG Cuts at: 2186 2539 3144 4716 2186 Size: 353 605 1572 2871 Fragments arranged by size: 2871 1572 605 353 ApaI G_GGCC'C Cuts at: 2422 2485 2422 Size: 63 5338 ApaLI G'TGCA_C Cuts at: 2306 3458 4614 2306 Size: 1152 1156 3093 Fragments arranged by size: 3093 1156 1152 ApoI r'AATT_y Cuts at: 1493 2710 2936 3088 5197 1493 Size: 1217 226 152 2109 1697 Fragments arranged by size: 2109 1697 1217 226 152 AseI AT'TA_AT Cuts at: 160 1032 3955 160 Size: 872 2923 1606 Fragments arranged by size: 2923 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5401 AvaI C'yCGr_G Cuts at: 1870 1943 2504 2618 2726 1870 Size: 73 561 114 108 4545 Fragments arranged by size: 4545 561 114 108 73 BamHI G'GATC_C Cuts at: 2731 3098 2731 Size: 367 5034 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2030 2579 2722 4177 618 Size: 325 217 627 243 549 143 1455 1842 Fragments arranged by size: 1842 1455 627 549 325 243 217 143 BanII G_rGCy'C Cuts at: 1391 2351 2422 2462 2485 2720 1391 Size: 960 71 40 23 235 4072 Fragments arranged by size: 4072 960 235 71 40 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2638 2667 928 Size: 971 739 29 3662 Fragments arranged by size: 3662 971 739 29 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3592 3626 1903 Size: 34 50 34 1571 34 3678 Fragments arranged by size: 3678 1571 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3293 4503 3293 Size: 1210 4191 BclI T'GATC_A Cuts at: 1548 1548 Size: 5401 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4012 136 Size: 107 122 71 631 605 2340 1525 Fragments arranged by size: 2340 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5396 5396 Size: 5401 BlpI GC'TnA_GC Cuts at: 2345 2818 2345 Size: 473 4928 Bme1580I G_kGCm'C Cuts at: 1790 2310 2422 2485 2582 3462 4618 1790 Size: 520 112 63 97 880 1156 2573 Fragments arranged by size: 2573 1156 880 520 112 97 63 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5401 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2534 2556 3132 Size: 416 53 83 399 24 1117 22 576 Cuts at: 3132 4090 442 Size: 958 1753 Fragments arranged by size: 1753 1117 958 576 416 399 83 53 24 22 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5401 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2365 2365 Size: 5401 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 2495 2615 2659 4061 1239 Size: 1013 243 120 44 1402 2579 Fragments arranged by size: 2579 1402 1013 243 120 44 Bpu10I CC'TnA_GC Cuts at: 2174 2174 Size: 5401 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3526 4391 4689 3526 Size: 865 298 4238 Fragments arranged by size: 4238 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2685 4064 882 Size: 617 1186 1379 2219 Fragments arranged by size: 2219 1379 1186 617 BsaAI yAC'GTr Cuts at: 493 1204 3192 493 Size: 711 1988 2702 Fragments arranged by size: 2702 1988 711 BsaBI GATnn'nnATC Cuts at: 5395 5395 Size: 5401 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3208 3590 4225 275 Size: 53 83 186 936 1675 382 635 1451 Fragments arranged by size: 1675 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3834 4232 4506 4653 5064 3834 Size: 398 274 147 411 4171 Fragments arranged by size: 4171 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5176 Fragments arranged by size: 5176 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2612 1186 Size: 1426 3975 BseYI C'CCAG_C Cuts at: 2008 2128 4604 5100 2008 Size: 120 2476 496 2309 Fragments arranged by size: 2476 2309 496 120 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2238 2371 1163 Size: 573 502 133 4193 Fragments arranged by size: 4193 573 502 133 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2086 2851 3612 4640 1540 Size: 275 186 85 765 761 1028 2301 Fragments arranged by size: 2301 1028 765 761 275 186 85 BsiHKAI G_wGCw'C Cuts at: 2310 2351 2625 3462 3547 4618 2310 Size: 41 274 837 85 1071 3093 Fragments arranged by size: 3093 1071 837 274 85 41 BsmI GAATG_Cn' Cuts at: 2912 3005 2912 Size: 93 5308 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2310 2351 2422 2462 2485 2582 2625 Size: 399 520 41 71 40 23 97 43 Cuts at: 2625 2720 3462 3547 4618 1391 Size: 95 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 520 399 97 95 85 71 43 41 40 23 BspEI T'CCGG_A Cuts at: 5064 5064 Size: 5401 BspHI T'CATG_A Cuts at: 3290 5020 3290 Size: 1730 3671 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2151 2673 2756 844 Size: 1307 522 83 3489 Fragments arranged by size: 3489 1307 522 83 BsrBI CCG'CTC Cuts at: 1394 1823 2563 3288 1394 Size: 429 740 725 3507 Fragments arranged by size: 3507 740 725 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3896 4070 65 Size: 1183 2648 174 1396 Fragments arranged by size: 2648 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2027 4045 4232 1700 Size: 110 217 2018 187 2869 Fragments arranged by size: 2869 2018 217 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5401 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5401 BssSI C'ACGA_G Cuts at: 3461 4473 3461 Size: 1012 4389 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5401 BstBI TT'CG_AA Cuts at: 4205 4205 Size: 5401 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5401 BstYI r'GATC_y Cuts at: 1972 2199 2731 3098 3483 3500 4952 5038 5050 Size: 227 532 367 385 17 1452 86 12 Cuts at: 5050 5396 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 532 385 367 346 227 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2123 2123 Size: 5401 BtgI C'CryG_G Cuts at: 513 1065 2149 2493 513 Size: 552 1084 344 3421 Fragments arranged by size: 3421 1084 552 344 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4662 Fragments arranged by size: 4662 683 56 BtsI GCAGTG_nn' Cuts at: 2917 3711 3731 2917 Size: 794 20 4587 Fragments arranged by size: 4587 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5401 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5366 DraI TTT'AAA Cuts at: 2705 2705 Size: 5401 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4408 809 Size: 507 3092 1802 Fragments arranged by size: 3092 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2068 2433 2848 Size: 54 1425 50 275 18 238 365 415 Cuts at: 2848 3737 5366 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 415 365 275 238 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2848 1537 Size: 275 1036 4090 Fragments arranged by size: 4090 1036 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3331 1353 Size: 1978 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3988 4362 4508 387 Size: 1064 138 2399 374 146 1280 Fragments arranged by size: 2399 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2349 2349 Size: 5401 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2495 2615 2659 3464 4061 4848 Size: 393 620 243 120 44 805 597 787 Cuts at: 4848 5116 1239 Size: 268 1524 Fragments arranged by size: 1524 805 787 620 597 393 268 243 120 44 EcoNI CCTnn'n_nnAGG Cuts at: 2597 2597 Size: 5401 EcoO109I rG'GnC_Cy Cuts at: 2247 2327 2482 2247 Size: 80 155 5166 Fragments arranged by size: 5166 155 80 EcoRI G'AATT_C Cuts at: 2710 3088 2710 Size: 378 5023 FspI TGC'GCA Cuts at: 3907 3907 Size: 5401 HaeII r_GCGC'y Cuts at: 1678 2171 4548 1678 Size: 493 2377 2531 Fragments arranged by size: 2531 2377 493 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4042 4074 4116 4148 1373 Size: 32 135 32 2470 32 42 32 2626 Fragments arranged by size: 2626 2470 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2745 2991 669 Size: 988 281 807 246 3079 Fragments arranged by size: 3079 988 807 281 246 HindIII A'AGCT_T Cuts at: 748 2587 748 Size: 1839 3562 HpaI GTT'AAC Cuts at: 2991 2991 Size: 5401 KpnI G_GTAC'C Cuts at: 2726 2726 Size: 5401 MfeI C'AATT_G Cuts at: 3000 3000 Size: 5401 MluI A'CGCG_T Cuts at: 5277 5277 Size: 5401 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2656 4515 4699 1305 Size: 1351 1859 184 2007 Fragments arranged by size: 2007 1859 1351 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2435 10 Size: 54 1425 343 603 2976 Fragments arranged by size: 2976 1425 603 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2036 2280 2337 3359 3718 3877 518 Size: 648 870 244 57 1022 359 159 2042 Fragments arranged by size: 2042 1022 870 648 359 244 159 57 MspA1I CmG'CkG Cuts at: 1606 2264 2539 2817 3494 4642 4887 1606 Size: 658 275 278 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 658 278 275 245 NaeI GCC'GGC Cuts at: 2029 2029 Size: 5401 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4849 NdeI CA'TA_TG Cuts at: 387 387 Size: 5401 NgoMIV G'CCGG_C Cuts at: 2027 2027 Size: 5401 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5401 NotI GC'GGCC_GC Cuts at: 2848 2848 Size: 5401 NspI r_CATG'y Cuts at: 1132 1229 2759 4304 1132 Size: 97 1530 1545 2229 Fragments arranged by size: 2229 1545 1530 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5401 PciI A'CATG_T Cuts at: 4300 4300 Size: 5401 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5401 PfoI T'CCnGG_A Cuts at: 2211 2592 2211 Size: 381 5020 PmeI GTTT'AAAC Cuts at: 2705 2705 Size: 5401 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3470 3502 4983 5015 3470 Size: 32 1481 32 3856 Fragments arranged by size: 3856 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2247 2327 2247 Size: 80 5321 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5401 PsiI TTA'TAA Cuts at: 2971 2971 Size: 5401 PspOMI G'GGCC_C Cuts at: 2418 2481 2418 Size: 63 5338 PstI C_TGCA'G Cuts at: 830 2535 2753 830 Size: 1705 218 3478 Fragments arranged by size: 3478 1705 218 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5401 PvuII CAG'CTG Cuts at: 2264 2264 Size: 5401 SacI G_AGCT'C Cuts at: 2351 2351 Size: 5401 SalI G'TCGA_C Cuts at: 1936 2743 1936 Size: 807 4594 SanDI GG'GwC_CC Cuts at: 2247 2327 2247 Size: 80 5321 SbfI CC_TGCA'GG Cuts at: 2753 2753 Size: 5401 ScaI AGT'ACT Cuts at: 3649 5361 3649 Size: 1712 3689 SfcI C'TryA_G Cuts at: 826 1045 2531 2749 3884 4565 4756 826 Size: 219 1486 218 1135 681 191 1471 Fragments arranged by size: 1486 1471 1135 681 219 218 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5401 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5401 SmaI CCC'GGG Cuts at: 1872 2728 1872 Size: 856 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2618 3505 4406 4668 820 Size: 197 926 675 887 901 262 1553 Fragments arranged by size: 1553 926 901 887 675 262 197 SnaBI TAC'GTA Cuts at: 493 3192 493 Size: 2699 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5401 SphI G_CATG'C Cuts at: 1132 2759 1132 Size: 1627 3774 SspI AAT'ATT Cuts at: 5 52 3325 5 Size: 47 3273 2081 Fragments arranged by size: 3273 2081 47 StuI AGG'CCT Cuts at: 1517 2099 2237 1517 Size: 582 138 4681 Fragments arranged by size: 4681 582 138 StyI C'CwwG_G Cuts at: 513 1065 2059 2840 513 Size: 552 994 781 3074 Fragments arranged by size: 3074 994 781 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2272 3428 3445 3598 3783 4228 325 Size: 1255 692 1156 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1156 692 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3647 5359 96 Size: 274 80 33 51 1443 1670 1712 138 Fragments arranged by size: 1712 1670 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5361 5393 5361 Size: 32 5369 Tth111I GACn'n_nGTC Cuts at: 1316 2642 1316 Size: 1326 4075 XbaI T'CTAG_A Cuts at: 2737 2737 Size: 5401 XhoI C'TCGA_G Cuts at: 1943 2618 1943 Size: 675 4726 XmaI C'CCGG_G Cuts at: 1870 2726 1870 Size: 856 4545 XmnI GAAnn'nnTTC Cuts at: 3530 3530 Size: 5401 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3209 276 Size: 53 83 186 936 1675 2468 Fragments arranged by size: 2468 1675 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI