################################## # NO CUT ENZYMES ( 201 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AciI 474 CCGC CCGC(-3/-1) BanI 236 GGCACC G^G[CT][GA]CC BbsI 32 GAAGAC GAAGAC(2/6) BpmI 134 CTCCAG CTGGAG(16/14) BpuAI 32 GAAGAC GAAGAC(2/6) BsaJI 289 CCTGGG C^C[ACGT][ACGT]GG BsrI 61 CCAGT ACTGG(1/-1) BssKI 289 CCTGG ^CC[ACGT]GG BstNI 289 CCTGG CC^[AT]GG Cfr13I 130 GGCCC G^G[ACGT]CC DpnII 100 GATC ^GATC DraI 457 TTTAAA TTT^AAA DraIII 343 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRII 289 CCTGG ^CC[AT]GG FauI 473 CCCGC CCCGC(4/6) HaeIII 130 GGCC GG^CC HinfI 400 GACTC G^A[ACGT]TC MboI 100 GATC ^GATC MvaI 289 CCTGG CC^[AT]GG MwoI 30 GCGAAGACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 100 GATC ^GATC PflMI 342 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 400 GACTC GAGTC(4/5) PvuII 37 CAGCTG CAG^CTG Sau3AI 100 GATC ^GATC Sau96I 130 GGCCC G^G[ACGT]CC ScrFI 289 CCTGG CC^[ACGT]GG SfaNI 418 GATGC GCATC(5/9) Van91I 342 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 319 CCAGAGGAACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 217 AAATTC [GA]^AATT[CT] 454 GAATTT AluI 8 AGCT AG^CT 38 AGCT ApoI 217 AAATTC [GA]^AATT[CT] 454 GAATTT BbvI 9 GCTGC GCAGC(8/12) 421 GCTGC BsiYI 61 CCAGTGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 342 CCACACTGTGG BslI 61 CCAGTGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 342 CCACACTGTGG BsmAI 398 GAGAC GTCTC(1/5) 496 GAGAC BspHI 282 TCATGA T^CATGA 359 TCATGA BspMI 405 ACCTGC ACCTGC(4/8) 79 GCAGGT Fnu4HI 9 GCTGC GC^[ACGT]GC 421 GCTGC HphI 259 GGTGA GGTGA(8/7) 403 TCACC ItaI 9 GCTGC GC^[ACGT]GC 421 GCTGC MslI 238 CACCCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 372 CATTTCTATG MspA1I 37 CAGCTG C[AC]G^C[GT]G 474 CCGCTG NlaIII 283 CATG CATG^ 360 CATG NlaIV 236 GGCACC GG[ACGT]^[ACGT]CC 332 GGCTCC PstI 10 CTGCAG CTGCA^G 77 CTGCAG RcaI 282 TCATGA T^CATGA 359 TCATGA SfcI 10 CTGCAG C^T[GA][CT]AG 77 CTGCAG TseI 9 GCTGC G^C[AT]GC 421 GCTGC Tsp509I 218 AATT ^AATT 455 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 6 enzymes) # ######################################### Cac8I MboII MnlI MseI Tru9I TspRI