(Circular) (Six-base) MAPSORT of: pFN21AE2250.seq Check: 6862 from: 1 to: 5215 pFN21AE2250 5215 bp With 182 enzymes: SgfI * October 27, 2011 14:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2419 2419 Size: 5215 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3025 278 Size: 53 83 186 936 1489 2468 Fragments arranged by size: 2468 1489 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2558 1937 Size: 621 4594 Acc65I G'GTAC_C Cuts at: 2536 2536 Size: 5215 AclI AA'CG_TT Cuts at: 1563 3342 3715 1563 Size: 1779 373 3063 Fragments arranged by size: 3063 1779 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3278 4662 4930 1632 Size: 1646 1384 268 1917 Fragments arranged by size: 1917 1646 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5018 AflIII A'CryG_T Cuts at: 1203 4114 5091 1203 Size: 2911 977 1327 Fragments arranged by size: 2911 1327 977 AgeI A'CCGG_T Cuts at: 4046 4046 Size: 5215 AhdI GACnn_n'nnGTC Cuts at: 3944 3944 Size: 5215 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5215 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5215 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2322 2354 2322 Size: 32 5183 AlwNI CAG_nnn'CTG Cuts at: 2958 4530 2958 Size: 1572 3643 ApaLI G'TGCA_C Cuts at: 3272 4428 3272 Size: 1156 4059 ApoI r'AATT_y Cuts at: 1493 2222 2459 2524 2750 2902 5011 1493 Size: 729 237 65 226 152 2109 1697 Fragments arranged by size: 2109 1697 729 237 226 152 65 AseI AT'TA_AT Cuts at: 160 1032 3769 160 Size: 872 2737 1606 Fragments arranged by size: 2737 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5215 AvaI C'yCGr_G Cuts at: 1870 1943 2540 1870 Size: 73 597 4545 Fragments arranged by size: 4545 597 73 BamHI G'GATC_C Cuts at: 2545 2912 2545 Size: 367 4848 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2241 2536 3991 618 Size: 325 217 627 454 295 1455 1842 Fragments arranged by size: 1842 1455 627 454 325 295 217 BanII G_rGCy'C Cuts at: 1391 2534 1391 Size: 1143 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2044 928 Size: 971 145 4099 Fragments arranged by size: 4099 971 145 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3406 3440 1903 Size: 34 1469 34 3678 Fragments arranged by size: 3678 1469 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3107 4317 3107 Size: 1210 4005 BclI T'GATC_A Cuts at: 1548 1548 Size: 5215 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3826 136 Size: 107 122 71 631 605 2154 1525 Fragments arranged by size: 2154 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5210 5210 Size: 5215 BlpI GC'TnA_GC Cuts at: 2632 2632 Size: 5215 Bme1580I G_kGCm'C Cuts at: 1790 3276 4432 1790 Size: 1486 1156 2573 Fragments arranged by size: 2573 1486 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5215 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2946 3904 442 Size: 416 53 83 399 24 1529 958 1753 Fragments arranged by size: 1753 1529 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5215 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2124 3875 1239 Size: 885 1751 2579 Fragments arranged by size: 2579 1751 885 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3340 4205 4503 3340 Size: 865 298 4052 Fragments arranged by size: 4052 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3878 882 Size: 617 2379 2219 Fragments arranged by size: 2379 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3006 493 Size: 711 1802 2702 Fragments arranged by size: 2702 1802 711 BsaBI GATnn'nnATC Cuts at: 5209 5209 Size: 5215 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3022 3404 4039 275 Size: 53 83 186 936 1489 382 635 1451 Fragments arranged by size: 1489 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3648 4046 4320 4467 4878 3648 Size: 398 274 147 411 3985 Fragments arranged by size: 3985 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2003 2033 2322 2352 1178 Size: 30 165 30 600 30 289 30 4041 Fragments arranged by size: 4041 600 289 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5215 BseYI C'CCAG_C Cuts at: 4418 4914 4418 Size: 496 4719 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4642 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2665 3426 4454 1540 Size: 275 186 664 761 1028 2301 Fragments arranged by size: 2301 1028 761 664 275 186 BsiHKAI G_wGCw'C Cuts at: 3276 3361 4432 3276 Size: 85 1071 4059 Fragments arranged by size: 4059 1071 85 BsmI GAATG_Cn' Cuts at: 2726 2819 2726 Size: 93 5122 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2534 3276 3361 4432 1391 Size: 399 744 742 85 1071 2174 Fragments arranged by size: 2174 1071 744 742 399 85 BspEI T'CCGG_A Cuts at: 4878 4878 Size: 5215 BspHI T'CATG_A Cuts at: 2287 2364 3104 4834 2287 Size: 77 740 1730 2668 Fragments arranged by size: 2668 1730 740 77 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2075 2419 2570 844 Size: 1231 344 151 3489 Fragments arranged by size: 3489 1231 344 151 BsrBI CCG'CTC Cuts at: 1394 1823 3102 1394 Size: 429 1279 3507 Fragments arranged by size: 3507 1279 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3710 3884 65 Size: 1183 2462 174 1396 Fragments arranged by size: 2462 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3859 4046 1700 Size: 110 2049 187 2869 Fragments arranged by size: 2869 2049 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5215 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5215 BssSI C'ACGA_G Cuts at: 3275 4287 3275 Size: 1012 4203 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5215 BstBI TT'CG_AA Cuts at: 4019 4019 Size: 5215 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5215 BstYI r'GATC_y Cuts at: 1972 2545 2912 3297 3314 4766 4852 4864 5210 Size: 573 367 385 17 1452 86 12 346 Cuts at: 5210 1972 Size: 1977 Fragments arranged by size: 1977 1452 573 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4663 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4476 Fragments arranged by size: 4476 683 56 BtsI GCAGTG_nn' Cuts at: 2024 2731 3525 3545 2024 Size: 707 794 20 3694 Fragments arranged by size: 3694 794 707 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5215 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5180 DraI TTT'AAA Cuts at: 2464 2519 2464 Size: 55 5160 DraIII CAC_nnn'GTG Cuts at: 2353 2353 Size: 5215 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4222 809 Size: 507 2906 1802 Fragments arranged by size: 2906 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2662 3551 5180 Size: 54 1425 50 275 18 832 889 1629 Cuts at: 5180 8 Size: 43 Fragments arranged by size: 1629 1425 889 832 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2662 1537 Size: 275 850 4090 Fragments arranged by size: 4090 850 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3145 1353 Size: 1792 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3802 4176 4322 387 Size: 1064 138 2213 374 146 1280 Fragments arranged by size: 2213 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2124 3278 3875 4662 4930 1239 Size: 393 492 1154 597 787 268 1524 Fragments arranged by size: 1524 1154 787 597 492 393 268 EcoRI G'AATT_C Cuts at: 2524 2902 2524 Size: 378 4837 FspI TGC'GCA Cuts at: 3721 3721 Size: 5215 HaeII r_GCGC'y Cuts at: 1678 4362 1678 Size: 2684 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 2106 2138 3856 Size: 32 135 32 431 32 71 32 1718 Cuts at: 3856 3888 3930 3962 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1718 431 135 71 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2559 2805 669 Size: 988 281 621 246 3079 Fragments arranged by size: 3079 988 621 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5215 HpaI GTT'AAC Cuts at: 2805 2805 Size: 5215 KpnI G_GTAC'C Cuts at: 2540 2540 Size: 5215 MfeI C'AATT_G Cuts at: 2814 2814 Size: 5215 MluI A'CGCG_T Cuts at: 5091 5091 Size: 5215 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2230 4329 4513 1305 Size: 925 2099 184 2007 Fragments arranged by size: 2099 2007 925 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3393 Fragments arranged by size: 3393 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2247 2381 3173 3532 3691 518 Size: 648 1081 134 792 359 159 2042 Fragments arranged by size: 2042 1081 792 648 359 159 134 MspA1I CmG'CkG Cuts at: 1606 2044 2481 2631 3308 4456 4701 1606 Size: 438 437 150 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 438 437 245 150 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4663 NdeI CA'TA_TG Cuts at: 387 387 Size: 5215 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5215 NotI GC'GGCC_GC Cuts at: 2662 2662 Size: 5215 NspI r_CATG'y Cuts at: 1132 1229 2573 4118 1132 Size: 97 1344 1545 2229 Fragments arranged by size: 2229 1545 1344 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5215 PciI A'CATG_T Cuts at: 4114 4114 Size: 5215 PflMI CCAn_nnn'nTGG Cuts at: 1108 2353 1108 Size: 1245 3970 PmeI GTTT'AAAC Cuts at: 2519 2519 Size: 5215 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2322 2354 3284 3316 4797 4829 2322 Size: 32 930 32 1481 32 2708 Fragments arranged by size: 2708 1481 930 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5215 PsiI TTA'TAA Cuts at: 2785 2785 Size: 5215 PstI C_TGCA'G Cuts at: 830 2019 2086 2567 830 Size: 1189 67 481 3478 Fragments arranged by size: 3478 1189 481 67 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5215 PvuII CAG'CTG Cuts at: 2044 2044 Size: 5215 SalI G'TCGA_C Cuts at: 1936 2557 1936 Size: 621 4594 SbfI CC_TGCA'GG Cuts at: 2567 2567 Size: 5215 ScaI AGT'ACT Cuts at: 3463 5175 3463 Size: 1712 3503 SfcI C'TryA_G Cuts at: 826 1045 2015 2082 2563 3698 4379 4570 826 Size: 219 970 67 481 1135 681 191 1471 Fragments arranged by size: 1471 1135 970 681 481 219 191 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5215 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5215 SmaI CCC'GGG Cuts at: 1872 2542 1872 Size: 670 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3319 4220 4482 820 Size: 197 926 1376 901 262 1553 Fragments arranged by size: 1553 1376 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3006 493 Size: 2513 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5215 SphI G_CATG'C Cuts at: 1132 2573 1132 Size: 1441 3774 SspI AAT'ATT Cuts at: 5 52 3139 5 Size: 47 3087 2081 Fragments arranged by size: 3087 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5215 StyI C'CwwG_G Cuts at: 513 1065 2654 513 Size: 552 1589 3074 Fragments arranged by size: 3074 1589 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2259 3242 3259 3412 3597 4042 325 Size: 1255 679 983 17 153 185 445 1498 Fragments arranged by size: 1498 1255 983 679 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3461 5173 96 Size: 274 80 33 51 1443 1484 1712 138 Fragments arranged by size: 1712 1484 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5175 5207 5175 Size: 32 5183 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5215 XbaI T'CTAG_A Cuts at: 2551 2551 Size: 5215 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2331 2331 Size: 5215 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5215 XmaI C'CCGG_G Cuts at: 1870 2540 1870 Size: 670 4545 XmnI GAAnn'nnTTC Cuts at: 3344 3344 Size: 5215 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3023 276 Size: 53 83 186 936 1489 2468 Fragments arranged by size: 2468 1489 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI