(Circular) (Six-base) MAPSORT of: pFN21AE0744.seq Check: 5344 from: 1 to: 5977 pFN21AE0744 5977 bp With 182 enzymes: SgfI * March 28, 2012 12:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2407 3232 2407 Size: 825 5152 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2151 3787 278 Size: 53 83 186 936 615 1636 2468 Fragments arranged by size: 2468 1636 936 615 186 83 53 AccI GT'mk_AC Cuts at: 1937 2217 3320 1937 Size: 280 1103 4594 Fragments arranged by size: 4594 1103 280 Acc65I G'GTAC_C Cuts at: 3298 3298 Size: 5977 AclI AA'CG_TT Cuts at: 1563 4104 4477 1563 Size: 2541 373 3063 Fragments arranged by size: 3063 2541 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2124 2267 2297 4040 5424 5692 1632 Size: 492 143 30 1743 1384 268 1917 Fragments arranged by size: 1917 1743 1384 492 268 143 30 AfeI AGC'GCT Cuts at: 2462 2885 2462 Size: 423 5554 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5780 AflIII A'CryG_T Cuts at: 1203 2709 4876 5853 1203 Size: 1506 2167 977 1327 Fragments arranged by size: 2167 1506 1327 977 AgeI A'CCGG_T Cuts at: 4808 4808 Size: 5977 AhdI GACnn_n'nnGTC Cuts at: 4706 4706 Size: 5977 AleI CACnn'nnGTG Cuts at: 1166 2702 1166 Size: 1536 4441 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5977 AlwNI CAG_nnn'CTG Cuts at: 2027 2119 2476 3720 5292 2027 Size: 92 357 1244 1572 2712 Fragments arranged by size: 2712 1572 1244 357 92 ApaI G_GGCC'C Cuts at: 3201 3201 Size: 5977 ApaLI G'TGCA_C Cuts at: 2516 4034 5190 2516 Size: 1518 1156 3303 Fragments arranged by size: 3303 1518 1156 ApoI r'AATT_y Cuts at: 1493 3286 3512 3664 5773 1493 Size: 1793 226 152 2109 1697 Fragments arranged by size: 2109 1793 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4531 160 Size: 872 3499 1606 Fragments arranged by size: 3499 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 AvaI C'yCGr_G Cuts at: 1870 1943 2534 2903 3002 3038 3173 3302 1870 Size: 73 591 369 99 36 135 129 4545 Fragments arranged by size: 4545 591 369 135 129 99 73 36 BamHI G'GATC_C Cuts at: 3307 3674 3307 Size: 367 5610 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2081 2189 2633 2650 2777 Size: 325 217 627 294 108 444 17 127 Cuts at: 2777 2847 3043 3121 3139 3145 3163 3298 4753 Size: 70 196 78 18 6 18 135 1455 Cuts at: 4753 618 Size: 1842 Fragments arranged by size: 1842 1455 627 444 325 294 217 196 135 127 108 78 70 18 18 17 6 BanII G_rGCy'C Cuts at: 1391 2442 2865 2904 2955 3180 3201 3296 1391 Size: 1051 423 39 51 225 21 95 4072 Fragments arranged by size: 4072 1051 423 225 95 51 39 21 BbeI G_GCGC'C Cuts at: 2654 2781 2851 3047 2654 Size: 127 70 196 5584 Fragments arranged by size: 5584 196 127 70 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2112 928 Size: 971 213 4793 Fragments arranged by size: 4793 971 213 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4168 4202 1903 Size: 34 2231 34 3678 Fragments arranged by size: 3678 2231 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3869 5079 3869 Size: 1210 4767 BclI T'GATC_A Cuts at: 1548 1548 Size: 5977 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2607 2670 4588 Size: 107 122 71 631 605 935 63 1918 Cuts at: 4588 136 Size: 1525 Fragments arranged by size: 1918 1525 935 631 605 122 107 71 63 BglII A'GATC_T Cuts at: 3267 5972 3267 Size: 2705 3272 BlpI GC'TnA_GC Cuts at: 3394 3394 Size: 5977 Bme1580I G_kGCm'C Cuts at: 1790 2094 2228 2520 3126 3156 3174 3201 4038 Size: 304 134 292 606 30 18 27 837 Cuts at: 4038 5194 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 837 606 304 292 134 30 27 18 BmgBI CAC'GTC Cuts at: 1318 2350 2710 2958 1318 Size: 1032 360 248 4337 Fragments arranged by size: 4337 1032 360 248 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3708 4666 442 Size: 416 53 83 399 24 2291 958 1753 Fragments arranged by size: 2291 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2305 2918 4637 1239 Size: 1066 613 1719 2579 Fragments arranged by size: 2579 1719 1066 613 Bpu10I CC'TnA_GC Cuts at: 2028 2028 Size: 5977 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2927 3041 4102 4967 5265 2927 Size: 114 1061 865 298 3639 Fragments arranged by size: 3639 1061 865 298 114 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4640 882 Size: 617 3141 2219 Fragments arranged by size: 3141 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3768 493 Size: 711 2564 2702 Fragments arranged by size: 2702 2564 711 BsaBI GATnn'nnATC Cuts at: 5971 5971 Size: 5977 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2148 2651 2771 2778 Size: 53 83 186 936 615 503 120 7 Cuts at: 2778 2784 2848 3044 3784 4166 4801 275 Size: 6 64 196 740 382 635 1451 Fragments arranged by size: 1451 936 740 635 615 503 382 196 186 120 83 64 53 7 6 BsaWI w'CCGG_w Cuts at: 4410 4808 5082 5229 5640 4410 Size: 398 274 147 411 4747 Fragments arranged by size: 4747 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2300 2330 2756 2786 1178 Size: 30 165 30 897 30 426 30 4369 Fragments arranged by size: 4369 897 426 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2308 2810 1186 Size: 1122 502 4353 Fragments arranged by size: 4353 1122 502 BseYI C'CCAG_C Cuts at: 2179 2402 2476 2917 5180 5676 2179 Size: 223 74 441 2263 496 2480 Fragments arranged by size: 2480 2263 496 441 223 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2489 1163 Size: 573 753 4651 Fragments arranged by size: 4651 753 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2557 3208 3220 3427 4188 5216 Size: 275 186 556 651 12 207 761 1028 Cuts at: 5216 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 651 556 275 207 186 12 BsiHKAI G_wGCw'C Cuts at: 2520 2730 2745 2955 4038 4123 5194 2520 Size: 210 15 210 1083 85 1071 3303 Fragments arranged by size: 3303 1083 1071 210 210 85 15 BsmI GAATG_Cn' Cuts at: 3488 3581 3488 Size: 93 5884 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2228 2442 2520 2730 2745 2865 Size: 399 304 134 214 78 210 15 120 Cuts at: 2865 2904 2955 3126 3156 3174 3180 3201 3296 Size: 39 51 171 30 18 6 21 95 Cuts at: 3296 4038 4123 5194 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 304 214 210 171 134 120 95 85 78 51 39 30 21 18 15 6 BspEI T'CCGG_A Cuts at: 5640 5640 Size: 5977 BspHI T'CATG_A Cuts at: 3866 5596 3866 Size: 1730 4247 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2057 2407 3232 3332 844 Size: 1213 350 825 100 3489 Fragments arranged by size: 3489 1213 825 350 100 BsrBI CCG'CTC Cuts at: 1394 1823 2040 2631 3055 3081 3864 1394 Size: 429 217 591 424 26 783 3507 Fragments arranged by size: 3507 783 591 429 424 217 26 BsrDI GCAATG_nn' Cuts at: 65 1248 4472 4646 65 Size: 1183 3224 174 1396 Fragments arranged by size: 3224 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2084 2747 2973 3136 3142 3160 4621 Size: 110 274 663 226 163 6 18 1461 Cuts at: 4621 4808 1700 Size: 187 2869 Fragments arranged by size: 2869 1461 663 274 226 187 163 110 18 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5977 BssHII G'CGCG_C Cuts at: 1925 2988 2990 1925 Size: 1063 2 4912 Fragments arranged by size: 4912 1063 2 BssSI C'ACGA_G Cuts at: 2948 4037 5049 2948 Size: 1089 1012 3876 Fragments arranged by size: 3876 1089 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2515 3247 794 Size: 1721 732 3524 Fragments arranged by size: 3524 1721 732 BstBI TT'CG_AA Cuts at: 4781 4781 Size: 5977 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5977 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5977 BstYI r'GATC_y Cuts at: 1972 3267 3307 3674 4059 4076 5528 5614 5626 Size: 1295 40 367 385 17 1452 86 12 Cuts at: 5626 5972 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1295 385 367 346 86 40 17 12 Bsu36I CC'TnA_GG Cuts at: 2445 2655 2445 Size: 210 5767 BtgI C'CryG_G Cuts at: 513 1065 2759 2911 3255 513 Size: 552 1694 152 344 3235 Fragments arranged by size: 3235 1694 552 344 152 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5238 Fragments arranged by size: 5238 683 56 BtsI GCAGTG_nn' Cuts at: 2849 3029 3493 4287 4307 2849 Size: 180 464 794 20 4519 Fragments arranged by size: 4519 794 464 180 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5942 DraI TTT'AAA Cuts at: 3281 3281 Size: 5977 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2213 2375 4984 809 Size: 507 897 162 2609 1802 Fragments arranged by size: 2609 1802 897 507 162 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2136 2580 3205 Size: 54 1425 50 275 18 306 444 625 Cuts at: 3205 3217 3252 3258 3424 4313 5942 8 Size: 12 35 6 166 889 1629 43 Fragments arranged by size: 1629 1425 889 625 444 306 275 166 54 50 43 35 18 12 6 EagI C'GGCC_G Cuts at: 1537 1812 3205 3217 3424 1537 Size: 275 1393 12 207 4090 Fragments arranged by size: 4090 1393 275 207 12 EarI CTCTTCn'nnn_ Cuts at: 1353 3907 1353 Size: 2554 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4564 4938 5084 387 Size: 1064 138 2975 374 146 1280 Fragments arranged by size: 2975 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2953 2953 Size: 5977 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2124 2267 2297 2305 2918 4040 4637 Size: 393 492 143 30 8 613 1122 597 Cuts at: 4637 5424 5692 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1122 787 613 597 492 393 268 143 30 8 EcoNI CCTnn'n_nnAGG Cuts at: 2291 2291 Size: 5977 EcoO109I rG'GnC_Cy Cuts at: 2116 2407 3197 3198 2116 Size: 291 790 1 4895 Fragments arranged by size: 4895 790 291 1 EcoRI G'AATT_C Cuts at: 3286 3664 3286 Size: 378 5599 FspI TGC'GCA Cuts at: 2873 4483 2873 Size: 1610 4367 HaeII r_GCGC'y Cuts at: 1678 2464 2489 2654 2781 2851 2887 2911 3047 Size: 786 25 165 127 70 36 24 136 Cuts at: 3047 5124 1678 Size: 2077 2531 Fragments arranged by size: 2531 2077 786 165 136 127 70 36 25 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2756 2788 2942 2974 4618 Size: 32 135 32 1184 32 154 32 1644 Cuts at: 4618 4650 4692 4724 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1644 1184 154 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2206 3321 3567 669 Size: 988 281 268 1115 246 3079 Fragments arranged by size: 3079 1115 988 281 268 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5977 HpaI GTT'AAC Cuts at: 3567 3567 Size: 5977 KasI G'GCGC_C Cuts at: 2650 2777 2847 3043 2650 Size: 127 70 196 5584 Fragments arranged by size: 5584 196 127 70 KpnI G_GTAC'C Cuts at: 3302 3302 Size: 5977 MfeI C'AATT_G Cuts at: 3576 3576 Size: 5977 MluI A'CGCG_T Cuts at: 5853 5853 Size: 5977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5091 5275 1305 Size: 3786 184 2007 Fragments arranged by size: 3786 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3254 10 Size: 54 1425 343 1422 2733 Fragments arranged by size: 2733 1425 1422 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2237 2702 3935 4294 4453 518 Size: 648 843 228 465 1233 359 159 2042 Fragments arranged by size: 2042 1233 843 648 465 359 228 159 MspA1I CmG'CkG Cuts at: 1606 2476 2512 2815 2913 3087 3393 4070 5218 Size: 870 36 303 98 174 306 677 1148 Cuts at: 5218 5463 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 870 677 306 303 245 174 98 36 NaeI GCC'GGC Cuts at: 2975 2975 Size: 5977 NarI GG'CG_CC Cuts at: 2651 2778 2848 3044 2651 Size: 127 70 196 5584 Fragments arranged by size: 5584 196 127 70 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5425 NdeI CA'TA_TG Cuts at: 387 387 Size: 5977 NgoMIV G'CCGG_C Cuts at: 2973 2973 Size: 5977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5977 NotI GC'GGCC_GC Cuts at: 3424 3424 Size: 5977 NspI r_CATG'y Cuts at: 1132 1229 2113 2878 3335 4880 1132 Size: 97 884 765 457 1545 2229 Fragments arranged by size: 2229 1545 884 765 457 97 PasI CC'CwG_GG Cuts at: 1348 2072 3193 1348 Size: 724 1121 4132 Fragments arranged by size: 4132 1121 724 PciI A'CATG_T Cuts at: 4876 4876 Size: 5977 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5977 PfoI T'CCnGG_A Cuts at: 2172 2172 Size: 5977 PmeI GTTT'AAAC Cuts at: 3281 3281 Size: 5977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4046 4078 5559 5591 4046 Size: 32 1481 32 4432 Fragments arranged by size: 4432 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2407 2407 Size: 5977 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5977 PsiI TTA'TAA Cuts at: 3547 3547 Size: 5977 PspOMI G'GGCC_C Cuts at: 3197 3197 Size: 5977 PspXI vC'TCGA_Gb Cuts at: 3038 3038 Size: 5977 PstI C_TGCA'G Cuts at: 830 3329 830 Size: 2499 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5977 PvuII CAG'CTG Cuts at: 2476 2512 2476 Size: 36 5941 RsrII CG'GwC_CG Cuts at: 3210 3210 Size: 5977 SacI G_AGCT'C Cuts at: 2955 2955 Size: 5977 SacII CC_GC'GG Cuts at: 2914 2914 Size: 5977 SalI G'TCGA_C Cuts at: 1936 3319 1936 Size: 1383 4594 SbfI CC_TGCA'GG Cuts at: 3329 3329 Size: 5977 ScaI AGT'ACT Cuts at: 4225 5937 4225 Size: 1712 4265 SexAI A'CCwGG_T Cuts at: 2208 2208 Size: 5977 SfcI C'TryA_G Cuts at: 826 1045 3325 4460 5141 5332 826 Size: 219 2280 1135 681 191 1471 Fragments arranged by size: 2280 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2652 2779 2849 3045 2652 Size: 127 70 196 5584 Fragments arranged by size: 5584 196 127 70 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5977 SmaI CCC'GGG Cuts at: 1872 3004 3304 1872 Size: 1132 300 4545 Fragments arranged by size: 4545 1132 300 SmlI C'TyrA_G Cuts at: 820 1017 1943 2942 3038 3056 4081 4982 5244 Size: 197 926 999 96 18 1025 901 262 Cuts at: 5244 820 Size: 1553 Fragments arranged by size: 1553 1025 999 926 901 262 197 96 18 SnaBI TAC'GTA Cuts at: 493 3768 493 Size: 3275 2702 SpeI A'CTAG_T Cuts at: 152 2167 152 Size: 2015 3962 SphI G_CATG'C Cuts at: 1132 3335 1132 Size: 2203 3774 SspI AAT'ATT Cuts at: 5 52 3901 5 Size: 47 3849 2081 Fragments arranged by size: 3849 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5977 StyI C'CwwG_G Cuts at: 513 1065 2922 3416 513 Size: 552 1857 494 3074 Fragments arranged by size: 3074 1857 552 494 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3089 3227 4004 4021 4174 4359 4804 Size: 1255 1509 138 777 17 153 185 445 Cuts at: 4804 325 Size: 1498 Fragments arranged by size: 1509 1498 1255 777 445 185 153 138 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4223 5935 96 Size: 274 80 33 51 1443 2246 1712 138 Fragments arranged by size: 2246 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5937 5969 5937 Size: 32 5945 Tth111I GACn'n_nGTC Cuts at: 1316 2351 1316 Size: 1035 4942 XbaI T'CTAG_A Cuts at: 3313 3313 Size: 5977 XhoI C'TCGA_G Cuts at: 1943 3038 1943 Size: 1095 4882 XmaI C'CCGG_G Cuts at: 1870 3002 3302 1870 Size: 1132 300 4545 Fragments arranged by size: 4545 1132 300 XmnI GAAnn'nnTTC Cuts at: 2282 4106 2282 Size: 1824 4153 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2149 3785 276 Size: 53 83 186 936 615 1636 2468 Fragments arranged by size: 2468 1636 936 615 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI SanDI SapI SfiI SrfI SwaI XcmI