(Linear) (Six-base) MAPSORT of: KIEE3200.seq Check: 2522 from: 1 to: 6327 KIEE3200.orf With 182 enzymes: SgfI * December 27, 2011 16:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 3873 5164 5305 6327 Size: 3873 1291 141 1022 Fragments arranged by size: 3873 1291 1022 141 AatII G_ACGT'C Cuts at: 0 983 6327 Size: 983 5344 AccI GT'mk_AC Cuts at: 0 290 2639 4778 6327 Size: 290 2349 2139 1549 Fragments arranged by size: 2349 2139 1549 290 Acc65I G'GTAC_C Cuts at: 0 2423 4196 6327 Size: 2423 1773 2131 Fragments arranged by size: 2423 2131 1773 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 532 1027 1424 1637 1807 2582 2627 2833 Size: 532 495 397 213 170 775 45 206 Cuts at: 2833 2890 3679 4270 5723 5879 6327 Size: 57 789 591 1453 156 448 Fragments arranged by size: 1453 789 775 591 532 495 448 397 213 206 170 156 57 45 AfeI AGC'GCT Cuts at: 0 68 5660 6148 6327 Size: 68 5592 488 179 Fragments arranged by size: 5592 488 179 68 AflIII A'CryG_T Cuts at: 0 1688 2411 6327 Size: 1688 723 3916 Fragments arranged by size: 3916 1688 723 AgeI A'CCGG_T Cuts at: 0 218 734 6327 Size: 218 516 5593 Fragments arranged by size: 5593 516 218 AleI CACnn'nnGTG Cuts at: 0 3856 5047 6327 Size: 3856 1191 1280 Fragments arranged by size: 3856 1280 1191 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3745 5065 6327 Size: 3745 1320 1262 Fragments arranged by size: 3745 1320 1262 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1072 1104 6327 Size: 1072 32 5223 Fragments arranged by size: 5223 1072 32 AlwNI CAG_nnn'CTG Cuts at: 0 1612 2865 2913 4185 4293 5379 6120 6327 Size: 1612 1253 48 1272 108 1086 741 207 Fragments arranged by size: 1612 1272 1253 1086 741 207 108 48 ApaI G_GGCC'C Cuts at: 0 150 2233 3868 4462 4479 5696 6327 Size: 150 2083 1635 594 17 1217 631 Fragments arranged by size: 2083 1635 1217 631 594 150 17 ApaLI G'TGCA_C Cuts at: 0 2074 6327 Size: 2074 4253 ApoI r'AATT_y Cuts at: 0 248 641 1012 1148 2794 4105 4149 5673 Size: 248 393 371 136 1646 1311 44 1524 Cuts at: 5673 6327 Size: 654 Fragments arranged by size: 1646 1524 1311 654 393 371 248 136 44 AscI GG'CGCG_CC Cuts at: 0 5586 6327 Size: 5586 741 AvaI C'yCGr_G Cuts at: 0 162 188 5539 5570 6049 6327 Size: 162 26 5351 31 479 278 Fragments arranged by size: 5351 479 278 162 31 26 BamHI G'GATC_C Cuts at: 0 501 4754 6317 6327 Size: 501 4253 1563 10 Fragments arranged by size: 4253 1563 501 10 BanI G'GyrC_C Cuts at: 0 992 2423 2438 3068 3343 4196 4436 5550 Size: 992 1431 15 630 275 853 240 1114 Cuts at: 5550 5575 5629 6327 Size: 25 54 698 Fragments arranged by size: 1431 1114 992 853 698 630 275 240 54 25 15 BanII G_rGCy'C Cuts at: 0 47 150 337 578 705 1355 2233 2709 Size: 47 103 187 241 127 650 878 476 Cuts at: 2709 3356 3560 3602 3868 3960 4462 4479 4655 Size: 647 204 42 266 92 502 17 176 Cuts at: 4655 5422 5696 5857 6058 6235 6254 6327 Size: 767 274 161 201 177 19 73 Fragments arranged by size: 878 767 650 647 502 476 274 266 241 204 201 187 177 176 161 127 103 92 73 47 42 19 17 BbeI G_GCGC'C Cuts at: 0 3072 4440 6327 Size: 3072 1368 1887 Fragments arranged by size: 3072 1887 1368 BbsI GAAGACnn'nnnn_ Cuts at: 0 1259 1795 2216 5216 5474 5495 5510 5756 Size: 1259 536 421 3000 258 21 15 246 Cuts at: 5756 5786 5867 6327 Size: 30 81 460 Fragments arranged by size: 3000 1259 536 460 421 258 246 81 30 21 15 BbvCI CC'TCA_GC Cuts at: 0 835 6327 Size: 835 5492 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3544 3578 3739 3773 6327 Size: 3544 34 161 34 2554 Fragments arranged by size: 3544 2554 161 34 34 BclI T'GATC_A Cuts at: 0 446 1859 1919 6327 Size: 446 1413 60 4408 Fragments arranged by size: 4408 1413 446 60 BglI GCCn_nnn'nGGC Cuts at: 0 463 2551 2853 5058 5573 5596 6327 Size: 463 2088 302 2205 515 23 731 Fragments arranged by size: 2205 2088 731 515 463 302 23 BglII A'GATC_T Cuts at: 0 5231 6327 Size: 5231 1096 BlpI GC'TnA_GC Cuts at: 0 796 5827 6327 Size: 796 5031 500 Fragments arranged by size: 5031 796 500 Bme1580I G_kGCm'C Cuts at: 0 150 2078 2192 2233 2441 3762 3868 4462 Size: 150 1928 114 41 208 1321 106 594 Cuts at: 4462 4479 5054 5578 5632 5696 6215 6327 Size: 17 575 524 54 64 519 112 Fragments arranged by size: 1928 1321 594 575 524 519 208 150 114 112 106 64 54 41 17 BmrI ACTGGGnnnn_n' Cuts at: 0 1802 2368 2671 6327 Size: 1802 566 303 3656 Fragments arranged by size: 3656 1802 566 303 BmtI G_CTAG'C Cuts at: 0 907 6327 Size: 907 5420 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1074 2574 6327 Size: 1074 1500 3753 Fragments arranged by size: 3753 1500 1074 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 58 103 196 562 719 1058 2168 Size: 31 27 45 93 366 157 339 1110 Cuts at: 2168 2809 3280 3301 3350 3451 4162 4712 4855 Size: 641 471 21 49 101 711 550 143 Cuts at: 4855 5164 5206 5383 5401 5530 5647 5831 6097 Size: 309 42 177 18 129 117 184 266 Cuts at: 6097 6124 6142 6201 6327 Size: 27 18 59 126 Fragments arranged by size: 1110 711 641 550 471 366 339 309 266 184 177 157 143 129 126 117 101 93 59 49 45 42 31 27 27 21 18 18 Bpu10I CC'TnA_GC Cuts at: 0 835 1315 1806 5062 6327 Size: 835 480 491 3256 1265 Fragments arranged by size: 3256 1265 835 491 480 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 982 3364 6327 Size: 982 2382 2963 Fragments arranged by size: 2963 2382 982 BsaI GGTCTCn'nnnn_ Cuts at: 0 578 2014 5608 6327 Size: 578 1436 3594 719 Fragments arranged by size: 3594 1436 719 578 BsaAI yAC'GTr Cuts at: 0 4535 6327 Size: 4535 1792 BsaBI GATnn'nnATC Cuts at: 0 2966 6327 Size: 2966 3361 BsaHI Gr'CG_yC Cuts at: 0 980 1375 2519 3069 4437 5471 5621 6327 Size: 980 395 1144 550 1368 1034 150 706 Fragments arranged by size: 1368 1144 1034 980 706 550 395 150 BsaWI w'CCGG_w Cuts at: 0 218 734 1751 2135 3617 6140 6327 Size: 218 516 1017 384 1482 2523 187 Fragments arranged by size: 2523 1482 1017 516 384 218 187 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 4 34 1056 1086 2617 2647 3253 3283 Size: 4 30 1022 30 1531 30 606 30 Cuts at: 3283 6327 Size: 3044 Fragments arranged by size: 3044 1531 1022 606 30 30 30 30 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 196 712 768 1063 1318 1402 1456 1459 Size: 196 516 56 295 255 84 54 3 Cuts at: 1459 1464 1759 2523 2716 3223 4434 5069 5182 Size: 5 295 764 193 507 1211 635 113 Cuts at: 5182 5293 6327 Size: 111 1034 Fragments arranged by size: 1211 1034 764 635 516 507 295 295 255 196 193 113 111 84 56 54 5 3 BseYI C'CCAG_C Cuts at: 0 616 704 825 846 1523 2177 2702 3339 Size: 616 88 121 21 677 654 525 637 Cuts at: 3339 3569 4455 5594 5683 5739 6276 6327 Size: 230 886 1139 89 56 537 51 Fragments arranged by size: 1139 886 677 654 637 616 537 525 230 121 89 88 56 51 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 108 610 4542 5025 5425 6327 Size: 108 502 3932 483 400 902 Fragments arranged by size: 3932 902 502 483 400 108 BsiEI CG_ry'CG Cuts at: 0 2527 5952 5956 6327 Size: 2527 3425 4 371 Fragments arranged by size: 3425 2527 371 4 BsiHKAI G_wGCw'C Cuts at: 0 578 802 1355 2078 3560 3960 4306 6058 Size: 578 224 553 723 1482 400 346 1752 Cuts at: 6058 6327 Size: 269 Fragments arranged by size: 1752 1482 723 578 553 400 346 269 224 BsmI GAATG_Cn' Cuts at: 0 1208 3000 4592 6327 Size: 1208 1792 1592 1735 Fragments arranged by size: 1792 1735 1592 1208 BsmBI CGTCTCn'nnnn_ Cuts at: 0 79 814 1680 6327 Size: 79 735 866 4647 Fragments arranged by size: 4647 866 735 79 Bsp1286I G_dGCh'C Cuts at: 0 47 150 337 578 705 802 1355 2078 Size: 47 103 187 241 127 97 553 723 Cuts at: 2078 2192 2233 2441 2709 3356 3560 3602 3762 Size: 114 41 208 268 647 204 42 160 Cuts at: 3762 3868 3960 4306 4462 4479 4655 5054 5422 Size: 106 92 346 156 17 176 399 368 Cuts at: 5422 5578 5632 5696 5857 6058 6215 6235 6254 Size: 156 54 64 161 201 157 20 19 Cuts at: 6254 6327 Size: 73 Fragments arranged by size: 723 647 553 399 368 346 268 241 208 204 201 187 176 161 160 157 156 156 127 114 106 103 97 92 73 64 54 47 42 41 20 19 17 BspEI T'CCGG_A Cuts at: 0 1751 2135 6140 6327 Size: 1751 384 4005 187 Fragments arranged by size: 4005 1751 384 187 BspHI T'CATG_A Cuts at: 0 4049 6029 6327 Size: 4049 1980 298 Fragments arranged by size: 4049 1980 298 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2115 3128 3509 3873 4917 4943 5164 5305 Size: 2115 1013 381 364 1044 26 221 141 Cuts at: 5305 6327 Size: 1022 Fragments arranged by size: 2115 1044 1022 1013 381 364 221 141 26 BsrBI CCG'CTC Cuts at: 0 1095 5077 5831 6327 Size: 1095 3982 754 496 Fragments arranged by size: 3982 1095 754 496 BsrDI GCAATG_nn' Cuts at: 0 3830 3846 6327 Size: 3830 16 2481 Fragments arranged by size: 3830 2481 16 BsrFI r'CCGG_y Cuts at: 0 77 218 554 734 1319 3053 4433 4597 Size: 77 141 336 180 585 1734 1380 164 Cuts at: 4597 5951 6327 Size: 1354 376 Fragments arranged by size: 1734 1380 1354 585 376 336 180 164 141 77 BsrGI T'GTAC_A Cuts at: 0 406 2867 6327 Size: 406 2461 3460 Fragments arranged by size: 3460 2461 406 BssHII G'CGCG_C Cuts at: 0 5401 5586 6327 Size: 5401 185 741 Fragments arranged by size: 5401 741 185 BssSI C'ACGA_G Cuts at: 0 2649 6295 6327 Size: 2649 3646 32 Fragments arranged by size: 3646 2649 32 BstAPI GCAn_nnn'nTGC Cuts at: 0 4556 5376 5448 6327 Size: 4556 820 72 879 Fragments arranged by size: 4556 879 820 72 BstXI CCAn_nnnn'nTGG Cuts at: 0 71 5508 6327 Size: 71 5437 819 Fragments arranged by size: 5437 819 71 BstYI r'GATC_y Cuts at: 0 501 935 2064 2138 3392 4754 5231 6317 Size: 501 434 1129 74 1254 1362 477 1086 Cuts at: 6317 6327 Size: 10 Fragments arranged by size: 1362 1254 1129 1086 501 477 434 74 10 Bsu36I CC'TnA_GG Cuts at: 0 4144 6327 Size: 4144 2183 BtgI C'CryG_G Cuts at: 0 2084 2456 2621 2945 3242 5252 5414 5956 Size: 2084 372 165 324 297 2010 162 542 Cuts at: 5956 6191 6236 6327 Size: 235 45 91 Fragments arranged by size: 2084 2010 542 372 324 297 235 165 162 91 45 BtsI GCAGTG_nn' Cuts at: 0 596 3130 3434 5396 6327 Size: 596 2534 304 1962 931 Fragments arranged by size: 2534 1962 931 596 304 DraI TTT'AAA Cuts at: 0 1017 4101 6327 Size: 1017 3084 2226 Fragments arranged by size: 3084 2226 1017 DraIII CAC_nnn'GTG Cuts at: 0 1958 5448 6327 Size: 1958 3490 879 Fragments arranged by size: 3490 1958 879 DrdI GACnn_nn'nnGTC Cuts at: 0 1051 3477 6327 Size: 1051 2426 2850 Fragments arranged by size: 2850 2426 1051 EaeI y'GGCC_r Cuts at: 0 2524 4010 4706 5832 5949 5953 5973 6327 Size: 2524 1486 696 1126 117 4 20 354 Fragments arranged by size: 2524 1486 1126 696 354 117 20 4 EagI C'GGCC_G Cuts at: 0 2524 5949 5953 6327 Size: 2524 3425 4 374 Fragments arranged by size: 3425 2524 374 4 EarI CTCTTCn'nnn_ Cuts at: 0 836 926 1613 3569 3911 3948 4053 4411 Size: 836 90 687 1956 342 37 105 358 Cuts at: 4411 4726 4847 5081 6184 6194 6327 Size: 315 121 234 1103 10 133 Fragments arranged by size: 1956 1103 836 687 358 342 315 234 133 121 105 90 37 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 4348 6327 Size: 4348 1979 EcoICRI GAG'CTC Cuts at: 0 576 1353 3558 3958 6056 6327 Size: 576 777 2205 400 2098 271 Fragments arranged by size: 2205 2098 777 576 400 271 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 58 103 196 532 562 719 1027 Size: 31 27 45 93 336 30 157 308 Cuts at: 1027 1058 1424 1637 1807 2168 2582 2627 2809 Size: 31 366 213 170 361 414 45 182 Cuts at: 2809 2833 2890 3280 3301 3350 3451 3679 4162 Size: 24 57 390 21 49 101 228 483 Cuts at: 4162 4270 4712 4855 5164 5206 5383 5401 5530 Size: 108 442 143 309 42 177 18 129 Cuts at: 5530 5647 5723 5831 5879 6097 6124 6142 6201 Size: 117 76 108 48 218 27 18 59 Cuts at: 6201 6327 Size: 126 Fragments arranged by size: 483 442 414 390 366 361 336 309 308 228 218 213 182 177 170 157 143 129 126 117 108 108 101 93 76 59 57 49 48 45 45 42 31 31 30 27 27 24 21 18 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 426 1999 3063 3223 4012 4521 6327 Size: 426 1573 1064 160 789 509 1806 Fragments arranged by size: 1806 1573 1064 789 509 426 160 EcoO109I rG'GnC_Cy Cuts at: 0 147 267 479 1478 2229 3666 3864 4459 Size: 147 120 212 999 751 1437 198 595 Cuts at: 4459 4476 5355 5724 6081 6117 6327 Size: 17 879 369 357 36 210 Fragments arranged by size: 1437 999 879 751 595 369 357 212 210 198 147 120 36 17 EcoRI G'AATT_C Cuts at: 0 4149 6327 Size: 4149 2178 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2487 2632 4440 6327 Size: 2487 145 1808 1887 Fragments arranged by size: 2487 1887 1808 145 FseI GG_CCGG'CC Cuts at: 0 5955 6327 Size: 5955 372 FspI TGC'GCA Cuts at: 0 4913 5287 6327 Size: 4913 374 1040 Fragments arranged by size: 4913 1040 374 HaeII r_GCGC'y Cuts at: 0 70 3072 3242 3489 4440 4890 5662 6150 Size: 70 3002 170 247 951 450 772 488 Cuts at: 6150 6327 Size: 177 Fragments arranged by size: 3002 951 772 488 450 247 177 170 70 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1056 1088 1512 1544 1911 1943 2632 2664 Size: 1056 32 424 32 367 32 689 32 Cuts at: 2664 3919 3951 4609 4641 6327 Size: 1255 32 658 32 1686 Fragments arranged by size: 1686 1255 1056 689 658 424 367 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 2640 3009 4431 4446 5010 6303 6327 Size: 2640 369 1422 15 564 1293 24 Fragments arranged by size: 2640 1422 1293 564 369 24 15 HindIII A'AGCT_T Cuts at: 0 781 2469 4117 4812 6327 Size: 781 1688 1648 695 1515 Fragments arranged by size: 1688 1648 1515 781 695 KasI G'GCGC_C Cuts at: 0 3068 4436 6327 Size: 3068 1368 1891 Fragments arranged by size: 3068 1891 1368 KpnI G_GTAC'C Cuts at: 0 2427 4200 6327 Size: 2427 1773 2127 Fragments arranged by size: 2427 2127 1773 MfeI C'AATT_G Cuts at: 0 4211 6327 Size: 4211 2116 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2 1412 3927 4020 4586 6327 Size: 2 1410 2515 93 566 1741 Fragments arranged by size: 2515 1741 1410 566 93 2 MscI TGG'CCA Cuts at: 0 5975 6327 Size: 5975 352 MslI CAynn'nnrTG Cuts at: 0 301 772 1034 1735 3856 4741 4900 5047 Size: 301 471 262 701 2121 885 159 147 Cuts at: 5047 5635 6327 Size: 588 692 Fragments arranged by size: 2121 885 701 692 588 471 301 262 159 147 MspA1I CmG'CkG Cuts at: 0 108 2256 2811 3768 5039 5071 5184 5594 Size: 108 2148 555 957 1271 32 113 410 Cuts at: 5594 5758 5764 5826 6327 Size: 164 6 62 501 Fragments arranged by size: 2148 1271 957 555 501 410 164 113 108 62 32 6 NaeI GCC'GGC Cuts at: 0 79 1321 3055 4599 5953 6327 Size: 79 1242 1734 1544 1354 374 Fragments arranged by size: 1734 1544 1354 1242 374 79 NarI GG'CG_CC Cuts at: 0 3069 4437 6327 Size: 3069 1368 1890 Fragments arranged by size: 3069 1890 1368 NcoI C'CATG_G Cuts at: 0 2084 3242 5414 6236 6327 Size: 2084 1158 2172 822 91 Fragments arranged by size: 2172 2084 1158 822 91 NdeI CA'TA_TG Cuts at: 0 302 6327 Size: 302 6025 NgoMIV G'CCGG_C Cuts at: 0 77 1319 3053 4597 5951 6327 Size: 77 1242 1734 1544 1354 376 Fragments arranged by size: 1734 1544 1354 1242 376 77 NheI G'CTAG_C Cuts at: 0 903 6327 Size: 903 5424 NspI r_CATG'y Cuts at: 0 2415 3907 6327 Size: 2415 1492 2420 Fragments arranged by size: 2420 2415 1492 PasI CC'CwG_GG Cuts at: 0 3670 6327 Size: 3670 2657 PciI A'CATG_T Cuts at: 0 2411 6327 Size: 2411 3916 PflMI CCAn_nnn'nTGG Cuts at: 0 4278 4455 4578 6120 6327 Size: 4278 177 123 1542 207 Fragments arranged by size: 4278 1542 207 177 123 PfoI T'CCnGG_A Cuts at: 0 212 539 2352 4178 4757 5360 5663 6327 Size: 212 327 1813 1826 579 603 303 664 Fragments arranged by size: 1826 1813 664 603 579 327 303 212 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1764 1796 6327 Size: 1764 32 4531 Fragments arranged by size: 4531 1764 32 PpuMI rG'GwC_Cy Cuts at: 0 267 1478 3666 6081 6327 Size: 267 1211 2188 2415 246 Fragments arranged by size: 2415 2188 1211 267 246 PshAI GACnn'nnGTC Cuts at: 0 979 1259 2032 6327 Size: 979 280 773 4295 Fragments arranged by size: 4295 979 773 280 PspOMI G'GGCC_C Cuts at: 0 146 2229 3864 4458 4475 5692 6327 Size: 146 2083 1635 594 17 1217 635 Fragments arranged by size: 2083 1635 1217 635 594 146 17 PstI C_TGCA'G Cuts at: 0 3125 3224 3767 5161 5763 6089 6327 Size: 3125 99 543 1394 602 326 238 Fragments arranged by size: 3125 1394 602 543 326 238 99 PvuII CAG'CTG Cuts at: 0 2256 5184 5594 5826 6327 Size: 2256 2928 410 232 501 Fragments arranged by size: 2928 2256 501 410 232 SacI G_AGCT'C Cuts at: 0 578 1355 3560 3960 6058 6327 Size: 578 777 2205 400 2098 269 Fragments arranged by size: 2205 2098 777 578 400 269 SalI G'TCGA_C Cuts at: 0 2638 6327 Size: 2638 3689 SanDI GG'GwC_CC Cuts at: 0 267 1478 6327 Size: 267 1211 4849 Fragments arranged by size: 4849 1211 267 SapI GCTCTTCn'nnn_ Cuts at: 0 836 3569 3948 5081 6184 6327 Size: 836 2733 379 1133 1103 143 Fragments arranged by size: 2733 1133 1103 836 379 143 SexAI A'CCwGG_T Cuts at: 0 3790 6327 Size: 3790 2537 SfcI C'TryA_G Cuts at: 0 205 2932 3121 3220 3763 4521 5157 5759 Size: 205 2727 189 99 543 758 636 602 Cuts at: 5759 6085 6327 Size: 326 242 Fragments arranged by size: 2727 758 636 602 543 326 242 205 189 99 SfoI GGC'GCC Cuts at: 0 3070 4438 6327 Size: 3070 1368 1889 Fragments arranged by size: 3070 1889 1368 SmaI CCC'GGG Cuts at: 0 164 5572 6327 Size: 164 5408 755 Fragments arranged by size: 5408 755 164 SmlI C'TyrA_G Cuts at: 0 997 3379 6327 Size: 997 2382 2948 Fragments arranged by size: 2948 2382 997 SphI G_CATG'C Cuts at: 0 3907 6327 Size: 3907 2420 SspI AAT'ATT Cuts at: 0 3528 6327 Size: 3528 2799 StuI AGG'CCT Cuts at: 0 2554 3985 4399 6039 6168 6327 Size: 2554 1431 414 1640 129 159 Fragments arranged by size: 2554 1640 1431 414 159 129 StyI C'CwwG_G Cuts at: 0 908 1547 2084 2193 3242 3854 5225 5303 Size: 908 639 537 109 1049 612 1371 78 Cuts at: 5303 5414 5431 5688 6163 6236 6257 6327 Size: 111 17 257 475 73 21 70 Fragments arranged by size: 1371 1049 908 639 612 537 475 257 111 109 78 73 70 21 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 777 991 1544 1592 3331 3608 3626 5632 Size: 777 214 553 48 1739 277 18 2006 Cuts at: 5632 5647 6327 Size: 15 680 Fragments arranged by size: 2006 1739 777 680 553 277 214 48 18 15 TatI w'GTAC_w Cuts at: 0 406 1280 2867 3284 4373 6327 Size: 406 874 1587 417 1089 1954 Fragments arranged by size: 1954 1587 1089 874 417 406 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3253 3285 3443 3475 4688 4720 6327 Size: 3253 32 158 32 1213 32 1607 Fragments arranged by size: 3253 1607 1213 158 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 28 6327 Size: 28 6299 XbaI T'CTAG_A Cuts at: 0 2269 6327 Size: 2269 4058 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 946 1368 1980 2454 3875 4662 6327 Size: 946 422 612 474 1421 787 1665 Fragments arranged by size: 1665 1421 946 787 612 474 422 XmaI C'CCGG_G Cuts at: 0 162 5570 6327 Size: 162 5408 757 Fragments arranged by size: 5408 757 162 XmnI GAAnn'nnTTC Cuts at: 0 2954 4399 6327 Size: 2954 1445 1928 Fragments arranged by size: 2954 1928 1445 ZraI GAC'GTC Cuts at: 0 981 6327 Size: 981 5346 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AfeI AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SacI SalI SanDI SapI SexAI SfcI SfoI SmaI SmlI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI Enzymes that do not cut: AclI AflII AhdI AseI AsiSI AvrII BaeI BciVI BfrBI BmgBI BsiWI BstBI BstEII BstZ17I BtgZI ClaI CspCI EcoRV FspAI HpaI MluI NotI NruI NsiI PacI PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacII SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI SwaI XhoI