################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BbrPI BbvCI BcgI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmI BsoBI BspDI BspEI BspLU11I BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII ClaI CpoI DraI DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HgaI HindIII KpnI KspI MaeIII MamI MfeI MluI MluNI MroI MscI MunI MvnI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AcsI 322 GAATTC [GA]^AATT[CT] AcyI 762 GGCGCC G[GA]^CG[CT]C AocI 289 CCTCAGG CC^T[ACGT]AGG ApaI 722 GGGCCC GGGCC^C ApoI 322 GAATTC [GA]^AATT[CT] AspHI 4 GAGCAC G[AT]GC[AT]^C BamHI 554 GGATCC G^GATCC BanII 722 GGGCCC G[GA]GC[CT]^C BbeI 762 GGCGCC GGCGC^C BciVI 1051 GTATCC GTATCC(6/5) BclI 143 TGATCA T^GATCA BfaI 817 CTAG C^TAG BsaHI 762 GGCGCC G[GA]^CG[CT]C BsaI 414 GGTCTC GGTCTC(1/5) BsiHKAI 4 GAGCAC G[AT]GC[AT]^C BsmBI 649 CGTCTC CGTCTC(1/5) BspHI 737 TCATGA T^CATGA BspMI 463 ACCTGC ACCTGC(4/8) BsrBI 397 CCGCTC CCGCTC(-3/-3) BsrDI 804 CATTGC GCAATG(2/0) BsrFI 984 GCCGGC [GA]^CCGG[CT] BstAPI 202 GCACTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 750 CCACCTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 289 CCTCAGG CC^T[ACGT]AGG CfoI 763 GCGC GCG^C Cfr10I 984 GCCGGC [GA]^CCGG[CT] DrdI 217 GACATCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 636 GAAGAG CTCTTC(1/4) Eco81I 289 CCTCAGG CC^T[ACGT]AGG EcoRI 322 GAATTC G^AATTC EcoT14I 881 CCATGG C^C[AT][AT]GG FbaI 143 TGATCA T^GATCA HaeII 762 GGCGCC [GA]GCGC^[CT] HhaI 763 GCGC GCG^C Hin1I 762 GGCGCC G[GA]^CG[CT]C HinP1I 763 GCGC G^CGC HincII 363 GTTAAC GT[CT]^[GA]AC HindII 363 GTTAAC GT[CT]^[GA]AC HinfI 741 GATTC G^A[ACGT]TC HpaI 363 GTTAAC GTT^AAC KasI 762 GGCGCC G^GCGCC Ksp632I 636 GAAGAG CTCTTC(1/4) MaeI 817 CTAG C^TAG MaeII 542 ACGT A^CGT MseI 364 TTAA T^TAA NaeI 984 GCCGGC GCC^GGC NarI 762 GGCGCC GG^CGCC NcoI 881 CCATGG C^CATGG NdeI 345 CATATG CA^TATG NgoMIV 984 GCCGGC G^CCGGC NheI 816 GCTAGC G^CTAGC PpuMI 293 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 173 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 157 CAGCTG CAG^CTG RcaI 737 TCATGA T^CATGA SapI 636 GAAGAGC GCTCTTC(1/4) SexAI 575 ACCTGGT A^CC[AT]GGT SfcI 96 CTACAG C^T[GA][CT]AG SfoI 762 GGCGCC GGC^GCC StyI 881 CCATGG C^C[AT][AT]GG TfiI 741 GATTC G^A[AT]TC Tru9I 364 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 91 GTCTAC GT^[AC][GT]AC 571 GTCTAC BanI 579 GGTGCC G^G[CT][GA]CC 762 GGCGCC BcnI 55 CCCGG CC^[GC]GG 434 CCCGG BsmAI 415 GTCTC GTCTC(1/5) 650 GTCTC BsmFI 51 GTCCC GGGAC(10/14) 295 GTCCC Bsp1407I 620 TGTACA T^GTACA 930 TGTACA BsrGI 620 TGTACA T^GTACA 930 TGTACA BsrI 491 ACTGG ACTGG(1/-1) 1020 CCAGT BstNI 576 CCTGG CC^[AT]GG 974 CCTGG BstYI 554 GGATCC [GA]^GATC[CT] 977 GGATCT DdeI 290 CTCAG C^T[ACGT]AG 628 CTCAG DraII 293 AGGTCCC [GA]G^G[ACGT]CC[CT] 722 GGGCCCC EcoO109I 293 AGGTCCC [GA]G^G[ACGT]CC[CT] 722 GGGCCCC EcoRII 576 CCTGG ^CC[AT]GG 974 CCTGG MflI 554 GGATCC [GA]^GATC[CT] 977 GGATCT MspA1I 157 CAGCTG C[AC]G^C[GT]G 777 CCGCTG MvaI 576 CCTGG CC^[AT]GG 974 CCTGG NciI 55 CCCGG CC^[GC]GG 434 CCCGG NspI 278 ACATGC [GA]CATG^[CT] 812 ACATGC SmlI 400 CTCAAG C^T[CT][GA]AG 898 CTCAAG SspBI 620 TGTACA T^GTACA 930 TGTACA Tsp509I 323 AATT ^AATT 643 AATT XhoII 554 GGATCC [GA]^GATC[CT] 977 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BbsI BbvI BmyI BpuAI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I Cac8I Cfr13I DpnII DsaI FauI Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MboI MboII MnlI MslI MspI MwoI NdeII NlaIII NlaIV PflMI RsaI Sau3AI Sau96I ScrFI SfaNI TseI TspRI Van91I