(Circular) (Six-base) MAPSORT of: pFN21AE9429.seq Check: 2723 from: 1 to: 5764 pFN21AE9429 5764 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2477 2477 Size: 5764 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3574 278 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2097 2577 3107 1937 Size: 160 480 530 4594 Fragments arranged by size: 4594 530 480 160 Acc65I G'GTAC_C Cuts at: 3085 3085 Size: 5764 AclI AA'CG_TT Cuts at: 1563 3891 4264 1563 Size: 2328 373 3063 Fragments arranged by size: 3063 2328 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2538 3827 5211 5479 1632 Size: 906 1289 1384 268 1917 Fragments arranged by size: 1917 1384 1289 906 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5567 AflIII A'CryG_T Cuts at: 1203 4663 5640 1203 Size: 3460 977 1327 Fragments arranged by size: 3460 1327 977 AgeI A'CCGG_T Cuts at: 4595 4595 Size: 5764 AhdI GACnn_n'nnGTC Cuts at: 4493 4493 Size: 5764 AleI CACnn'nnGTG Cuts at: 1166 2537 1166 Size: 1371 4393 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2555 3029 1184 Size: 1371 474 3919 Fragments arranged by size: 3919 1371 474 AlwNI CAG_nnn'CTG Cuts at: 3507 5079 3507 Size: 1572 4192 ApaI G_GGCC'C Cuts at: 2731 2731 Size: 5764 ApaLI G'TGCA_C Cuts at: 3821 4977 3821 Size: 1156 4608 ApoI r'AATT_y Cuts at: 1493 2327 3073 3299 3451 5560 1493 Size: 834 746 226 152 2109 1697 Fragments arranged by size: 2109 1697 834 746 226 152 AseI AT'TA_AT Cuts at: 160 1032 4318 160 Size: 872 3286 1606 Fragments arranged by size: 3286 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 AvaI C'yCGr_G Cuts at: 1870 1943 3089 1870 Size: 73 1146 4545 Fragments arranged by size: 4545 1146 73 BamHI G'GATC_C Cuts at: 2559 3094 3461 2559 Size: 535 367 4862 Fragments arranged by size: 4862 535 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2584 2767 3085 4540 618 Size: 325 217 627 797 183 318 1455 1842 Fragments arranged by size: 1842 1455 797 627 325 318 217 183 BanII G_rGCy'C Cuts at: 1391 2731 3083 1391 Size: 1340 352 4072 Fragments arranged by size: 4072 1340 352 BbeI G_GCGC'C Cuts at: 2771 2771 Size: 5764 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2182 2526 2684 928 Size: 971 283 344 158 4008 Fragments arranged by size: 4008 971 344 283 158 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3955 3989 1903 Size: 34 2018 34 3678 Fragments arranged by size: 3678 2018 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3067 3656 4866 3067 Size: 589 1210 3965 Fragments arranged by size: 3965 1210 589 BclI T'GATC_A Cuts at: 1548 2148 1548 Size: 600 5164 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4375 136 Size: 107 122 71 631 605 2703 1525 Fragments arranged by size: 2703 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5759 5759 Size: 5764 BlpI GC'TnA_GC Cuts at: 3181 3181 Size: 5764 Bme1580I G_kGCm'C Cuts at: 1790 2091 2589 2731 3825 4981 1790 Size: 301 498 142 1094 1156 2573 Fragments arranged by size: 2573 1156 1094 498 301 142 BmgBI CAC'GTC Cuts at: 1318 2548 1318 Size: 1230 4534 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3495 4453 442 Size: 416 53 83 399 24 2078 958 1753 Fragments arranged by size: 2078 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2825 1056 Size: 1769 3995 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4424 1239 Size: 3185 2579 Bpu10I CC'TnA_GC Cuts at: 2633 2633 Size: 5764 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2390 2888 3889 4754 5052 2390 Size: 498 1001 865 298 3102 Fragments arranged by size: 3102 1001 865 498 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2425 4427 882 Size: 617 926 2002 2219 Fragments arranged by size: 2219 2002 926 617 BsaAI yAC'GTr Cuts at: 493 1204 3555 493 Size: 711 2351 2702 Fragments arranged by size: 2702 2351 711 BsaBI GATnn'nnATC Cuts at: 5758 5758 Size: 5764 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2768 3571 3953 4588 Size: 53 83 186 936 1235 803 382 635 Cuts at: 4588 275 Size: 1451 Fragments arranged by size: 1451 1235 936 803 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4197 4595 4869 5016 5427 4197 Size: 398 274 147 411 4534 Fragments arranged by size: 4534 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5539 Fragments arranged by size: 5539 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5764 BseYI C'CCAG_C Cuts at: 2160 2428 4967 5463 2160 Size: 268 2539 496 2461 Fragments arranged by size: 2539 2461 496 268 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5191 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3214 3975 5003 1540 Size: 275 186 1213 761 1028 2301 Fragments arranged by size: 2301 1213 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2013 3825 3910 4981 2013 Size: 1812 85 1071 2796 Fragments arranged by size: 2796 1812 1071 85 BsmI GAATG_Cn' Cuts at: 3275 3368 3275 Size: 93 5671 BsmBI CGTCTCn'nnnn_ Cuts at: 2660 2660 Size: 5764 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2091 2589 2731 3083 3825 3910 Size: 399 223 78 498 142 352 742 85 Cuts at: 3910 4981 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 498 399 352 223 142 85 78 BspEI T'CCGG_A Cuts at: 5427 5427 Size: 5764 BspHI T'CATG_A Cuts at: 2742 3653 5383 2742 Size: 911 1730 3123 Fragments arranged by size: 3123 1730 911 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2477 3119 844 Size: 1633 642 3489 Fragments arranged by size: 3489 1633 642 BsrBI CCG'CTC Cuts at: 1394 1823 2404 3651 1394 Size: 429 581 1247 3507 Fragments arranged by size: 3507 1247 581 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2808 4259 4433 65 Size: 1183 1560 1451 174 1396 Fragments arranged by size: 1560 1451 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2989 4408 4595 1700 Size: 110 1179 1419 187 2869 Fragments arranged by size: 2869 1419 1179 187 110 BsrGI T'GTAC_A Cuts at: 96 2625 2935 96 Size: 2529 310 2925 Fragments arranged by size: 2925 2529 310 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5764 BssSI C'ACGA_G Cuts at: 3824 4836 3824 Size: 1012 4752 BstAPI GCAn_nnn'nTGC Cuts at: 794 2213 794 Size: 1419 4345 BstBI TT'CG_AA Cuts at: 4568 4568 Size: 5764 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5764 BstXI CCAn_nnnn'nTGG Cuts at: 2762 2762 Size: 5764 BstYI r'GATC_y Cuts at: 1972 2559 2982 3094 3461 3846 3863 5315 5401 Size: 587 423 112 367 385 17 1452 86 Cuts at: 5401 5413 5759 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 587 423 385 367 346 112 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2295 2295 Size: 5764 BtgI C'CryG_G Cuts at: 513 1065 2004 2091 2659 2670 2886 513 Size: 552 939 87 568 11 216 3391 Fragments arranged by size: 3391 939 568 552 216 87 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5025 Fragments arranged by size: 5025 683 56 BtsI GCAGTG_nn' Cuts at: 2563 3001 3280 4074 4094 2563 Size: 438 279 794 20 4233 Fragments arranged by size: 4233 794 438 279 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5764 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5729 DraI TTT'AAA Cuts at: 3068 3068 Size: 5764 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2228 4771 809 Size: 507 912 2543 1802 Fragments arranged by size: 2543 1802 912 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3211 4100 5729 Size: 54 1425 50 275 18 1381 889 1629 Cuts at: 5729 8 Size: 43 Fragments arranged by size: 1629 1425 1381 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3211 1537 Size: 275 1399 4090 Fragments arranged by size: 4090 1399 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2636 3694 1353 Size: 1283 1058 3423 Fragments arranged by size: 3423 1283 1058 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4351 4725 4871 387 Size: 1064 138 2762 374 146 1280 Fragments arranged by size: 2762 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2538 3827 4424 5211 5479 1239 Size: 393 906 1289 597 787 268 1524 Fragments arranged by size: 1524 1289 906 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2299 2728 2299 Size: 429 5335 EcoRI G'AATT_C Cuts at: 2327 3073 3451 2327 Size: 746 378 4640 Fragments arranged by size: 4640 746 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2929 2929 Size: 5764 FspI TGC'GCA Cuts at: 4270 4270 Size: 5764 HaeII r_GCGC'y Cuts at: 1678 2771 4911 1678 Size: 1093 2140 2531 Fragments arranged by size: 2531 2140 1093 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4405 4437 4479 4511 1373 Size: 32 135 32 2833 32 42 32 2626 Fragments arranged by size: 2833 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2370 3108 3354 669 Size: 988 281 432 738 246 3079 Fragments arranged by size: 3079 988 738 432 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5764 HpaI GTT'AAC Cuts at: 2370 3354 2370 Size: 984 4780 KasI G'GCGC_C Cuts at: 2767 2767 Size: 5764 KpnI G_GTAC'C Cuts at: 3089 3089 Size: 5764 MfeI C'AATT_G Cuts at: 3363 3363 Size: 5764 MluI A'CGCG_T Cuts at: 5640 5640 Size: 5764 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2244 2829 4878 5062 1305 Size: 939 585 2049 184 2007 Fragments arranged by size: 2049 2007 939 585 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3942 Fragments arranged by size: 3942 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2288 2318 2537 2741 2760 3011 3722 Size: 648 1122 30 219 204 19 251 711 Cuts at: 3722 4081 4240 518 Size: 359 159 2042 Fragments arranged by size: 2042 1122 711 648 359 251 219 204 159 30 19 MspA1I CmG'CkG Cuts at: 1606 2164 2784 3180 3857 5005 5250 1606 Size: 558 620 396 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 620 558 396 245 NaeI GCC'GGC Cuts at: 2991 2991 Size: 5764 NarI GG'CG_CC Cuts at: 2768 2768 Size: 5764 NcoI C'CATG_G Cuts at: 513 1065 2004 2886 513 Size: 552 939 882 3391 Fragments arranged by size: 3391 939 882 552 NdeI CA'TA_TG Cuts at: 387 2351 387 Size: 1964 3800 NgoMIV G'CCGG_C Cuts at: 2989 2989 Size: 5764 NheI G'CTAG_C Cuts at: 1052 2821 1052 Size: 1769 3995 NotI GC'GGCC_GC Cuts at: 3211 3211 Size: 5764 NspI r_CATG'y Cuts at: 1132 1229 2287 2821 3122 4667 1132 Size: 97 1058 534 301 1545 2229 Fragments arranged by size: 2229 1545 1058 534 301 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5764 PciI A'CATG_T Cuts at: 4663 4663 Size: 5764 PflMI CCAn_nnn'nTGG Cuts at: 1108 2167 2511 2724 1108 Size: 1059 344 213 4148 Fragments arranged by size: 4148 1059 344 213 PmeI GTTT'AAAC Cuts at: 3068 3068 Size: 5764 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3833 3865 5346 5378 3833 Size: 32 1481 32 4219 Fragments arranged by size: 4219 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2299 2299 Size: 5764 PshAI GACnn'nnGTC Cuts at: 1613 2182 1613 Size: 569 5195 PsiI TTA'TAA Cuts at: 3334 3334 Size: 5764 PspOMI G'GGCC_C Cuts at: 2727 2727 Size: 5764 PstI C_TGCA'G Cuts at: 830 3116 830 Size: 2286 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5764 PvuII CAG'CTG Cuts at: 2164 2164 Size: 5764 SalI G'TCGA_C Cuts at: 1936 3106 1936 Size: 1170 4594 SapI GCTCTTCn'nnn_ Cuts at: 2636 2636 Size: 5764 SbfI CC_TGCA'GG Cuts at: 3116 3116 Size: 5764 ScaI AGT'ACT Cuts at: 4012 5724 4012 Size: 1712 4052 SexAI A'CCwGG_T Cuts at: 2580 2580 Size: 5764 SfcI C'TryA_G Cuts at: 826 1045 2101 3112 4247 4928 5119 826 Size: 219 1056 1011 1135 681 191 1471 Fragments arranged by size: 1471 1135 1056 1011 681 219 191 SfoI GGC'GCC Cuts at: 2769 2769 Size: 5764 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5764 SmaI CCC'GGG Cuts at: 1872 3091 1872 Size: 1219 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2405 2903 3868 4769 5031 820 Size: 197 926 462 498 965 901 262 1553 Fragments arranged by size: 1553 965 926 901 498 462 262 197 SnaBI TAC'GTA Cuts at: 493 3555 493 Size: 3062 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5764 SphI G_CATG'C Cuts at: 1132 3122 1132 Size: 1990 3774 SspI AAT'ATT Cuts at: 5 52 3688 5 Size: 47 3636 2081 Fragments arranged by size: 3636 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5764 StyI C'CwwG_G Cuts at: 513 1065 2004 2886 3203 513 Size: 552 939 882 317 3074 Fragments arranged by size: 3074 939 882 552 317 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3791 3808 3961 4146 4591 325 Size: 1255 2211 17 153 185 445 1498 Fragments arranged by size: 2211 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2625 2935 4010 Size: 274 80 33 51 1443 648 310 1075 Cuts at: 4010 5722 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1075 648 310 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2786 2818 5724 5756 2786 Size: 32 2906 32 2794 Fragments arranged by size: 2906 2794 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5764 XbaI T'CTAG_A Cuts at: 3100 3100 Size: 5764 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5764 XmaI C'CCGG_G Cuts at: 1870 3089 1870 Size: 1219 4545 XmnI GAAnn'nnTTC Cuts at: 3893 3893 Size: 5764 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3572 276 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI