################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BbsI BbvCI BcgI BclI BfaI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CpoI DdeI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeI MamI MfeI MluI MroI MseI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tru9I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AcsI 577 GAATTT [GA]^AATT[CT] AcyI 108 GACGCC G[GA]^CG[CT]C Alw44I 121 GTGCAC G^TGCAC ApaLI 121 GTGCAC G^TGCAC ApoI 577 GAATTT [GA]^AATT[CT] BalI 762 TGGCCA TGG^CCA BamHI 254 GGATCC G^GATCC BanII 418 GGGCTC G[GA]GC[CT]^C BciVI 15 GTATCC GTATCC(6/5) BglI 68 GCCTGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 683 AGATCT A^GATCT BsaHI 108 GACGCC G[GA]^CG[CT]C BsmAI 645 GAGAC GTCTC(1/5) BspDI 403 ATCGAT AT^CGAT BspMI 83 ACCTGC ACCTGC(4/8) BsrFI 735 GCCGGC [GA]^CCGG[CT] BsrI 758 CCAGT ACTGG(1/-1) CfoI 87 GCGC GCG^C Cfr10I 735 GCCGGC [GA]^CCGG[CT] ClaI 403 ATCGAT AT^CGAT EarI 750 GAAGAG CTCTTC(1/4) EcoNI 93 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 108 GACGC GACGC(5/10) HhaI 87 GCGC GCG^C Hin1I 108 GACGCC G[GA]^CG[CT]C HinP1I 87 GCGC G^CGC HinfI 220 GAATC G^A[ACGT]TC Ksp632I 750 GAAGAG CTCTTC(1/4) MaeII 191 ACGT A^CGT MluNI 762 TGGCCA TGG^CCA MscI 762 TGGCCA TGG^CCA MslI 114 CACTCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 735 GCCGGC GCC^GGC NgoMIV 735 GCCGGC G^CCGGC SmlI 436 CTCAAG C^T[CT][GA]AG StuI 130 AGGCCT AGG^CCT TfiI 220 GAATC G^A[AT]TC Tsp509I 578 AATT ^AATT ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 570 GTAC GT^AC 670 GTAC AspHI 121 GTGCAC G[AT]GC[AT]^C 459 GTGCTC AvaI 532 CCCGAG C^[CT]CG[GA]G 541 CCCGAG BcnI 73 CCCGG CC^[GC]GG 509 CCCGG BpmI 94 CTGGAG CTGGAG(16/14) 622 CTGGAG BsgI 526 CTGCAC GTGCAG(16/14) 745 GTGCAG BsiHKAI 121 GTGCAC G[AT]GC[AT]^C 459 GTGCTC BsoBI 532 CCCGAG C^[CT]CG[GA]G 541 CCCGAG BsrBI 103 GAGCGG CCGCTC(-3/-3) 544 GAGCGG EaeI 75 CGGCCA [CT]^GGCC[GA] 762 TGGCCA MaeIII 373 GTGAC ^GT[ACGT]AC 379 GTGAC MboII 303 GAAGA GAAGA(8/7) 750 GAAGA NciI 73 CCCGG CC^[GC]GG 509 CCCGG NlaIII 136 CATG CATG^ 384 CATG PstI 40 CTGCAG CTGCA^G 712 CTGCAG RsaI 570 GTAC GT^AC 670 GTAC SfaNI 492 GATGC GCATC(5/9) 56 GCATC TaqI 404 TCGA T^CGA 422 TCGA Tsp45I 373 GTGAC ^GT[GC]AC 379 GTGAC TthHB8I 404 TCGA T^CGA 422 TCGA XcmI 383 CCATGCGGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 464 CCAAGTTTGGGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AluI AlwI AlwNI AvaII BbvI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BssKI BstF5I BstNI BstYI Cac8I Cfr13I DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HpaII ItaI MboI MflI MnlI MspA1I MspI MvaI MwoI NdeII NlaIV PpuMI PvuII Sau3AI Sau96I ScrFI SfcI TseI TspRI XhoII