(Circular) (Six-base) MAPSORT of: pFN21AE2208.seq Check: 5593 from: 1 to: 5473 pFN21AE2208 5473 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3283 278 Size: 53 83 186 936 1747 2468 Fragments arranged by size: 2468 1747 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2816 1937 Size: 879 4594 Acc65I G'GTAC_C Cuts at: 2794 2794 Size: 5473 AclI AA'CG_TT Cuts at: 1563 3600 3973 1563 Size: 2037 373 3063 Fragments arranged by size: 3063 2037 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2327 2507 3536 4920 5188 1632 Size: 695 180 1029 1384 268 1917 Fragments arranged by size: 1917 1384 1029 695 268 180 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5276 AflIII A'CryG_T Cuts at: 1203 4372 5349 1203 Size: 3169 977 1327 Fragments arranged by size: 3169 1327 977 AgeI A'CCGG_T Cuts at: 4304 4304 Size: 5473 AhdI GACnn_n'nnGTC Cuts at: 4202 4202 Size: 5473 AleI CACnn'nnGTG Cuts at: 1166 2123 1166 Size: 957 4516 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5473 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2354 2386 2354 Size: 32 5441 AlwNI CAG_nnn'CTG Cuts at: 2381 2716 2731 3216 4788 2381 Size: 335 15 485 1572 3066 Fragments arranged by size: 3066 1572 485 335 15 ApaLI G'TGCA_C Cuts at: 2126 3530 4686 2126 Size: 1404 1156 2913 Fragments arranged by size: 2913 1404 1156 ApoI r'AATT_y Cuts at: 1493 2582 2782 3008 3160 5269 1493 Size: 1089 200 226 152 2109 1697 Fragments arranged by size: 2109 1697 1089 226 200 152 AseI AT'TA_AT Cuts at: 160 1032 4027 160 Size: 872 2995 1606 Fragments arranged by size: 2995 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5473 AvaI C'yCGr_G Cuts at: 1870 1943 2537 2546 2798 1870 Size: 73 594 9 252 4545 Fragments arranged by size: 4545 594 252 73 9 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2003 2036 2003 Size: 33 5440 BamHI G'GATC_C Cuts at: 2259 2803 3170 2259 Size: 544 367 4562 Fragments arranged by size: 4562 544 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2794 4249 618 Size: 325 217 627 1007 1455 1842 Fragments arranged by size: 1842 1455 1007 627 325 217 BanII G_rGCy'C Cuts at: 1391 2427 2792 1391 Size: 1036 365 4072 Fragments arranged by size: 4072 1036 365 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4502 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3664 3698 1903 Size: 34 50 34 1643 34 3678 Fragments arranged by size: 3678 1643 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2031 3365 4575 2031 Size: 1334 1210 2929 Fragments arranged by size: 2929 1334 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5473 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2079 4084 136 Size: 107 122 71 631 605 407 2005 1525 Fragments arranged by size: 2005 1525 631 605 407 122 107 71 BglII A'GATC_T Cuts at: 2688 5468 2688 Size: 2780 2693 BlpI GC'TnA_GC Cuts at: 2890 2890 Size: 5473 Bme1580I G_kGCm'C Cuts at: 1790 2130 2202 3534 4690 1790 Size: 340 72 1332 1156 2573 Fragments arranged by size: 2573 1332 1156 340 72 BmgBI CAC'GTC Cuts at: 1318 2197 1318 Size: 879 4594 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3204 4162 442 Size: 416 53 83 399 24 1787 958 1753 Fragments arranged by size: 1787 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5473 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2120 2648 4133 1239 Size: 881 528 1485 2579 Fragments arranged by size: 2579 1485 881 528 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2426 3598 4463 4761 2426 Size: 1172 865 298 3138 Fragments arranged by size: 3138 1172 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4136 882 Size: 617 2637 2219 Fragments arranged by size: 2637 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3264 493 Size: 711 2060 2702 Fragments arranged by size: 2702 2060 711 BsaBI GATnn'nnATC Cuts at: 5467 5467 Size: 5473 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2114 3280 3662 4297 Size: 53 83 186 936 581 1166 382 635 Cuts at: 4297 275 Size: 1451 Fragments arranged by size: 1451 1166 936 635 581 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3906 4304 4578 4725 5136 3906 Size: 398 274 147 411 4243 Fragments arranged by size: 4243 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2078 2104 2108 2134 2279 Size: 30 165 30 675 26 4 26 145 Cuts at: 2279 2309 2447 2450 2477 2480 2594 2624 1178 Size: 30 138 3 27 3 114 30 4027 Fragments arranged by size: 4027 675 165 145 138 114 30 30 30 30 27 26 26 4 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2120 2612 2615 2645 2675 1186 Size: 931 3 492 3 30 30 3984 Fragments arranged by size: 3984 931 492 30 30 3 3 BseYI C'CCAG_C Cuts at: 2229 2526 4676 5172 2229 Size: 297 2150 496 2530 Fragments arranged by size: 2530 2150 496 297 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2516 2771 1163 Size: 573 780 255 3865 Fragments arranged by size: 3865 780 573 255 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2923 3684 4712 1540 Size: 275 186 922 761 1028 2301 Fragments arranged by size: 2301 1028 922 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2130 2468 3534 3619 4690 2130 Size: 338 1066 85 1071 2913 Fragments arranged by size: 2913 1071 1066 338 85 BsmI GAATG_Cn' Cuts at: 2984 3077 2984 Size: 93 5380 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2130 2202 2427 2468 2792 3534 3619 Size: 399 340 72 225 41 324 742 85 Cuts at: 3619 4690 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 340 324 225 85 72 41 BspEI T'CCGG_A Cuts at: 5136 5136 Size: 5473 BspHI T'CATG_A Cuts at: 3362 5092 3362 Size: 1730 3743 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2097 2828 844 Size: 1253 731 3489 Fragments arranged by size: 3489 1253 731 BsrBI CCG'CTC Cuts at: 1394 1823 2110 2551 3360 1394 Size: 429 287 441 809 3507 Fragments arranged by size: 3507 809 441 429 287 BsrDI GCAATG_nn' Cuts at: 65 1248 3968 4142 65 Size: 1183 2720 174 1396 Fragments arranged by size: 2720 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2740 4117 4304 1700 Size: 110 930 1377 187 2869 Fragments arranged by size: 2869 1377 930 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5473 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5473 BssSI C'ACGA_G Cuts at: 3533 4545 3533 Size: 1012 4461 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5473 BstBI TT'CG_AA Cuts at: 4277 4277 Size: 5473 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5473 BstYI r'GATC_y Cuts at: 1972 2259 2355 2688 2803 3170 3555 3572 5024 Size: 287 96 333 115 367 385 17 1452 Cuts at: 5024 5110 5122 5468 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 333 287 115 96 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4921 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4734 Fragments arranged by size: 4734 683 56 BtsI GCAGTG_nn' Cuts at: 2989 3783 3803 2989 Size: 794 20 4659 Fragments arranged by size: 4659 794 20 ClaI AT'CG_AT Cuts at: 1554 2409 1554 Size: 855 4618 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5438 DraI TTT'AAA Cuts at: 2777 2777 Size: 5473 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4480 809 Size: 507 3164 1802 Fragments arranged by size: 3164 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2080 2767 2920 Size: 54 1425 50 275 18 250 687 153 Cuts at: 2920 3809 5438 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 687 275 250 153 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2920 1537 Size: 275 1108 4090 Fragments arranged by size: 4090 1108 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2750 3403 1353 Size: 1397 653 3423 Fragments arranged by size: 3423 1397 653 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2714 4060 4434 4580 387 Size: 1064 138 1125 1346 374 146 1280 Fragments arranged by size: 1346 1280 1125 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2120 2327 2507 2648 3536 4133 4920 Size: 393 488 207 180 141 888 597 787 Cuts at: 4920 5188 1239 Size: 268 1524 Fragments arranged by size: 1524 888 787 597 488 393 268 207 180 141 EcoNI CCTnn'n_nnAGG Cuts at: 2102 2102 Size: 5473 EcoO109I rG'GnC_Cy Cuts at: 2458 2521 2542 2668 2458 Size: 63 21 126 5263 Fragments arranged by size: 5263 126 63 21 EcoRI G'AATT_C Cuts at: 2782 3160 2782 Size: 378 5095 FspI TGC'GCA Cuts at: 3979 3979 Size: 5473 HaeII r_GCGC'y Cuts at: 1678 4620 1678 Size: 2942 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2104 2136 2279 2311 2450 Size: 32 135 32 532 32 143 32 139 Cuts at: 2450 2482 4114 4146 4188 4220 1373 Size: 32 1632 32 42 32 2626 Fragments arranged by size: 2626 1632 532 143 139 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2817 3063 669 Size: 988 281 879 246 3079 Fragments arranged by size: 3079 988 879 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5473 HpaI GTT'AAC Cuts at: 3063 3063 Size: 5473 KpnI G_GTAC'C Cuts at: 2798 2798 Size: 5473 MfeI C'AATT_G Cuts at: 3072 3072 Size: 5473 MluI A'CGCG_T Cuts at: 5349 5349 Size: 5473 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4587 4771 1305 Size: 3282 184 2007 Fragments arranged by size: 3282 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2769 10 Size: 54 1425 343 937 2714 Fragments arranged by size: 2714 1425 937 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2123 3431 3790 3949 518 Size: 648 957 1308 359 159 2042 Fragments arranged by size: 2042 1308 957 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 2179 2233 2329 2530 2716 2889 3566 Size: 444 129 54 96 201 186 173 677 Cuts at: 3566 4714 4959 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 444 245 201 186 173 129 96 54 NaeI GCC'GGC Cuts at: 2742 2742 Size: 5473 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4921 NdeI CA'TA_TG Cuts at: 387 387 Size: 5473 NgoMIV G'CCGG_C Cuts at: 2740 2740 Size: 5473 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5473 NotI GC'GGCC_GC Cuts at: 2920 2920 Size: 5473 NspI r_CATG'y Cuts at: 1132 1229 2831 4376 1132 Size: 97 1602 1545 2229 Fragments arranged by size: 2229 1602 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5473 PciI A'CATG_T Cuts at: 4372 4372 Size: 5473 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5473 PfoI T'CCnGG_A Cuts at: 2358 2358 Size: 5473 PmeI GTTT'AAAC Cuts at: 2777 2777 Size: 5473 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2327 2359 3542 3574 5055 5087 2327 Size: 32 1183 32 1481 32 2713 Fragments arranged by size: 2713 1481 1183 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2458 2521 2542 2668 2458 Size: 63 21 126 5263 Fragments arranged by size: 5263 126 63 21 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5473 PsiI TTA'TAA Cuts at: 3043 3043 Size: 5473 PstI C_TGCA'G Cuts at: 830 2049 2721 2825 830 Size: 1219 672 104 3478 Fragments arranged by size: 3478 1219 672 104 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5473 PvuII CAG'CTG Cuts at: 2050 2329 2530 2716 2050 Size: 279 201 186 4807 Fragments arranged by size: 4807 279 201 186 SalI G'TCGA_C Cuts at: 1936 2815 1936 Size: 879 4594 SbfI CC_TGCA'GG Cuts at: 2825 2825 Size: 5473 ScaI AGT'ACT Cuts at: 3721 5433 3721 Size: 1712 3761 SfcI C'TryA_G Cuts at: 826 1045 2045 2131 2347 2717 2821 3956 4637 Size: 219 1000 86 216 370 104 1135 681 Cuts at: 4637 4828 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1000 681 370 219 216 191 104 86 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5473 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5473 SmaI CCC'GGG Cuts at: 1872 2800 1872 Size: 928 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2441 3577 4478 4740 820 Size: 197 926 498 1136 901 262 1553 Fragments arranged by size: 1553 1136 926 901 498 262 197 SnaBI TAC'GTA Cuts at: 493 3264 493 Size: 2771 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5473 SphI G_CATG'C Cuts at: 1132 2831 1132 Size: 1699 3774 SspI AAT'ATT Cuts at: 5 52 3397 5 Size: 47 3345 2081 Fragments arranged by size: 3345 2081 47 StuI AGG'CCT Cuts at: 1517 2137 1517 Size: 620 4853 StyI C'CwwG_G Cuts at: 513 1065 2912 513 Size: 552 1847 3074 Fragments arranged by size: 3074 1847 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2602 3500 3517 3670 3855 4300 325 Size: 1255 1022 898 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1022 898 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2574 3719 5431 Size: 274 80 33 51 1443 597 1145 1712 Cuts at: 5431 96 Size: 138 Fragments arranged by size: 1712 1443 1145 597 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2447 2479 2594 2626 5433 5465 2447 Size: 32 115 32 2807 32 2455 Fragments arranged by size: 2807 2455 115 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5473 XbaI T'CTAG_A Cuts at: 2809 2809 Size: 5473 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2395 2476 2395 Size: 81 5392 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5473 XmaI C'CCGG_G Cuts at: 1870 2798 1870 Size: 928 4545 XmnI GAAnn'nnTTC Cuts at: 3602 3602 Size: 5473 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3281 276 Size: 53 83 186 936 1747 2468 Fragments arranged by size: 2468 1747 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI