(Linear) (Six-base) MAPSORT of: KIEE2208.seq Check: 615 from: 1 to: 768 KIEE2208.orf With 182 enzymes: SgfI * July 3, 2012 17:14 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 502 768 Size: 322 180 266 Fragments arranged by size: 322 266 180 AleI CACnn'nnGTG Cuts at: 0 118 768 Size: 118 650 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 349 381 768 Size: 349 32 387 Fragments arranged by size: 387 349 32 AlwNI CAG_nnn'CTG Cuts at: 0 376 711 726 768 Size: 376 335 15 42 Fragments arranged by size: 376 335 42 15 ApaLI G'TGCA_C Cuts at: 0 121 768 Size: 121 647 ApoI r'AATT_y Cuts at: 0 577 768 Size: 577 191 AvaI C'yCGr_G Cuts at: 0 532 541 768 Size: 532 9 227 Fragments arranged by size: 532 227 9 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 31 768 Size: 31 737 BamHI G'GATC_C Cuts at: 0 254 768 Size: 254 514 BanII G_rGCy'C Cuts at: 0 422 768 Size: 422 346 BciVI GTATCCnnnnn_n' Cuts at: 0 26 768 Size: 26 742 BglI GCCn_nnn'nGGC Cuts at: 0 74 768 Size: 74 694 BglII A'GATC_T Cuts at: 0 683 768 Size: 683 85 Bme1580I G_kGCm'C Cuts at: 0 125 197 768 Size: 125 72 571 Fragments arranged by size: 571 125 72 BmgBI CAC'GTC Cuts at: 0 192 768 Size: 192 576 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 115 643 768 Size: 115 528 125 Fragments arranged by size: 528 125 115 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 421 768 Size: 421 347 BsaHI Gr'CG_yC Cuts at: 0 109 768 Size: 109 659 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 73 99 103 129 274 304 442 445 Size: 73 26 4 26 145 30 138 3 Cuts at: 445 472 475 589 619 768 Size: 27 3 114 30 149 Fragments arranged by size: 149 145 138 114 73 30 30 27 26 26 4 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 112 115 607 610 640 670 768 Size: 112 3 492 3 30 30 98 Fragments arranged by size: 492 112 98 30 30 3 3 BseYI C'CCAG_C Cuts at: 0 224 521 768 Size: 224 297 247 Fragments arranged by size: 297 247 224 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 511 766 768 Size: 511 255 2 Fragments arranged by size: 511 255 2 BsiHKAI G_wGCw'C Cuts at: 0 125 463 768 Size: 125 338 305 Fragments arranged by size: 338 305 125 Bsp1286I G_dGCh'C Cuts at: 0 125 197 422 463 768 Size: 125 72 225 41 305 Fragments arranged by size: 305 225 125 72 41 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 92 768 Size: 92 676 BsrBI CCG'CTC Cuts at: 0 105 546 768 Size: 105 441 222 Fragments arranged by size: 441 222 105 BsrFI r'CCGG_y Cuts at: 0 735 768 Size: 735 33 BstYI r'GATC_y Cuts at: 0 254 350 683 768 Size: 254 96 333 85 Fragments arranged by size: 333 254 96 85 ClaI AT'CG_AT Cuts at: 0 404 768 Size: 404 364 EaeI y'GGCC_r Cuts at: 0 75 762 768 Size: 75 687 6 Fragments arranged by size: 687 75 6 EarI CTCTTCn'nnn_ Cuts at: 0 745 768 Size: 745 23 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 709 768 Size: 709 59 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 115 322 502 643 768 Size: 115 207 180 141 125 Fragments arranged by size: 207 180 141 125 115 EcoNI CCTnn'n_nnAGG Cuts at: 0 97 768 Size: 97 671 EcoO109I rG'GnC_Cy Cuts at: 0 453 516 537 663 768 Size: 453 63 21 126 105 Fragments arranged by size: 453 126 105 63 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 99 131 274 306 445 477 768 Size: 99 32 143 32 139 32 291 Fragments arranged by size: 291 143 139 99 32 32 32 MscI TGG'CCA Cuts at: 0 764 768 Size: 764 4 MslI CAynn'nnrTG Cuts at: 0 118 768 Size: 118 650 MspA1I CmG'CkG Cuts at: 0 45 174 228 324 525 711 768 Size: 45 129 54 96 201 186 57 Fragments arranged by size: 201 186 129 96 57 54 45 NaeI GCC'GGC Cuts at: 0 737 768 Size: 737 31 NgoMIV G'CCGG_C Cuts at: 0 735 768 Size: 735 33 PfoI T'CCnGG_A Cuts at: 0 353 768 Size: 353 415 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 322 354 768 Size: 322 32 414 Fragments arranged by size: 414 322 32 PpuMI rG'GwC_Cy Cuts at: 0 453 516 537 663 768 Size: 453 63 21 126 105 Fragments arranged by size: 453 126 105 63 21 PstI C_TGCA'G Cuts at: 0 44 716 768 Size: 44 672 52 Fragments arranged by size: 672 52 44 PvuII CAG'CTG Cuts at: 0 45 324 525 711 768 Size: 45 279 201 186 57 Fragments arranged by size: 279 201 186 57 45 SfcI C'TryA_G Cuts at: 0 40 126 342 712 768 Size: 40 86 216 370 56 Fragments arranged by size: 370 216 86 56 40 SmlI C'TyrA_G Cuts at: 0 436 768 Size: 436 332 StuI AGG'CCT Cuts at: 0 132 768 Size: 132 636 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 597 768 Size: 597 171 TatI w'GTAC_w Cuts at: 0 569 768 Size: 569 199 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 442 474 589 621 768 Size: 442 32 115 32 147 Fragments arranged by size: 442 147 115 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 390 471 768 Size: 390 81 297 Fragments arranged by size: 390 297 81 Enzymes that do cut: AcuI AleI AloI AlwNI ApaLI ApoI AvaI BaeI BamHI BanII BciVI BglI BglII Bme1580I BmgBI BpmI BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstYI ClaI EaeI EarI EciI Eco57MI EcoNI EcoO109I Hin4I MscI MslI MspA1I NaeI NgoMIV PfoI PpiI PpuMI PstI PvuII SfcI SmlI StuI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI ApaI AscI AseI AsiSI AvrII BanI BbeI BbsI BbvCI BcgI BclI BfrBI BlpI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI