(Circular) (Six-base) MAPSORT of: pFN21AE6380.seq Check: 4114 from: 1 to: 5656 pFN21AE6380 5656 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3466 278 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2999 1937 Size: 1062 4594 Acc65I G'GTAC_C Cuts at: 2035 2977 2035 Size: 942 4714 AclI AA'CG_TT Cuts at: 1563 3783 4156 1563 Size: 2220 373 3063 Fragments arranged by size: 3063 2220 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2061 2640 3719 5103 5371 1632 Size: 429 579 1079 1384 268 1917 Fragments arranged by size: 1917 1384 1079 579 429 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5459 AflIII A'CryG_T Cuts at: 1203 4555 5532 1203 Size: 3352 977 1327 Fragments arranged by size: 3352 1327 977 AgeI A'CCGG_T Cuts at: 4487 4487 Size: 5656 AhdI GACnn_n'nnGTC Cuts at: 2395 4385 2395 Size: 1990 3666 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5656 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2138 2655 1184 Size: 954 517 4185 Fragments arranged by size: 4185 954 517 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2040 2072 2040 Size: 32 5624 AlwNI CAG_nnn'CTG Cuts at: 2408 3399 4971 2408 Size: 991 1572 3093 Fragments arranged by size: 3093 1572 991 ApaLI G'TGCA_C Cuts at: 3713 4869 3713 Size: 1156 4500 ApoI r'AATT_y Cuts at: 1493 2359 2965 3191 3343 5452 1493 Size: 866 606 226 152 2109 1697 Fragments arranged by size: 2109 1697 866 606 226 152 AseI AT'TA_AT Cuts at: 160 1032 4210 160 Size: 872 3178 1606 Fragments arranged by size: 3178 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 AvaI C'yCGr_G Cuts at: 1870 1943 2178 2230 2981 1870 Size: 73 235 52 751 4545 Fragments arranged by size: 4545 751 235 73 52 BamHI G'GATC_C Cuts at: 2986 3353 2986 Size: 367 5289 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 2977 4432 618 Size: 325 217 627 248 942 1455 1842 Fragments arranged by size: 1842 1455 942 627 325 248 217 BanII G_rGCy'C Cuts at: 1391 2058 2385 2975 1391 Size: 667 327 590 4072 Fragments arranged by size: 4072 667 590 327 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4685 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3847 3881 1903 Size: 34 1910 34 3678 Fragments arranged by size: 3678 1910 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3548 4758 3548 Size: 1210 4446 BclI T'GATC_A Cuts at: 1548 1548 Size: 5656 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4267 136 Size: 107 122 71 631 605 2595 1525 Fragments arranged by size: 2595 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2529 2659 5651 2529 Size: 130 2992 2534 Fragments arranged by size: 2992 2534 130 BlpI GC'TnA_GC Cuts at: 2910 3073 2910 Size: 163 5493 Bme1580I G_kGCm'C Cuts at: 1790 2024 2065 2879 3717 4873 1790 Size: 234 41 814 838 1156 2573 Fragments arranged by size: 2573 1156 838 814 234 41 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5656 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3387 4345 442 Size: 416 53 83 399 24 1970 958 1753 Fragments arranged by size: 1970 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5656 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 2576 2588 2669 4316 1239 Size: 851 486 12 81 1647 2579 Fragments arranged by size: 2579 1647 851 486 81 12 Bpu10I CC'TnA_GC Cuts at: 2379 2379 Size: 5656 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2813 3781 4646 4944 2813 Size: 968 865 298 3525 Fragments arranged by size: 3525 968 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2168 4319 882 Size: 617 669 2151 2219 Fragments arranged by size: 2219 2151 669 617 BsaAI yAC'GTr Cuts at: 493 1204 3447 493 Size: 711 2243 2702 Fragments arranged by size: 2702 2243 711 BsaBI GATnn'nnATC Cuts at: 5650 5650 Size: 5656 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3463 3845 4480 275 Size: 53 83 186 936 1930 382 635 1451 Fragments arranged by size: 1930 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2649 4089 4487 4761 4908 5319 2649 Size: 1440 398 274 147 411 2986 Fragments arranged by size: 2986 1440 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2040 2070 1178 Size: 30 165 30 637 30 4764 Fragments arranged by size: 4764 637 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2186 2198 2252 1186 Size: 1000 12 54 4590 Fragments arranged by size: 4590 1000 54 12 BseYI C'CCAG_C Cuts at: 2698 4859 5355 2698 Size: 2161 496 2999 Fragments arranged by size: 2999 2161 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2134 2234 2401 1163 Size: 573 398 100 167 4418 Fragments arranged by size: 4418 573 398 167 100 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3106 3867 4895 1540 Size: 275 186 1105 761 1028 2301 Fragments arranged by size: 2301 1105 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2058 2385 3717 3802 4873 2058 Size: 327 1332 85 1071 2841 Fragments arranged by size: 2841 1332 1071 327 85 BsmI GAATG_Cn' Cuts at: 3167 3260 3167 Size: 93 5563 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2058 2065 2385 2879 2975 3717 Size: 399 234 34 7 320 494 96 742 Cuts at: 3717 3802 4873 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 494 399 320 234 96 85 34 7 BspEI T'CCGG_A Cuts at: 2649 5319 2649 Size: 2670 2986 BspHI T'CATG_A Cuts at: 3545 5275 3545 Size: 1730 3926 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3011 844 Size: 2167 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3543 1394 Size: 429 1720 3507 Fragments arranged by size: 3507 1720 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4151 4325 65 Size: 1183 2903 174 1396 Fragments arranged by size: 2903 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4300 4487 1700 Size: 110 2490 187 2869 Fragments arranged by size: 2869 2490 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5656 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5656 BssSI C'ACGA_G Cuts at: 3716 4728 3716 Size: 1012 4644 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5656 BstBI TT'CG_AA Cuts at: 4460 4460 Size: 5656 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5656 BstYI r'GATC_y Cuts at: 1972 2529 2659 2986 3353 3738 3755 5207 5293 Size: 557 130 327 367 385 17 1452 86 Cuts at: 5293 5305 5651 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 557 385 367 346 327 130 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2092 2253 2735 2809 513 Size: 552 1027 161 482 74 3360 Fragments arranged by size: 3360 1027 552 482 161 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4917 Fragments arranged by size: 4917 683 56 BtsI GCAGTG_nn' Cuts at: 2196 3172 3966 3986 2196 Size: 976 794 20 3866 Fragments arranged by size: 3866 976 794 20 ClaI AT'CG_AT Cuts at: 1554 2844 1554 Size: 1290 4366 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5621 DraI TTT'AAA Cuts at: 2960 2960 Size: 5656 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4663 809 Size: 507 3347 1802 Fragments arranged by size: 3347 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 2467 3103 Size: 54 1425 50 275 18 420 217 636 Cuts at: 3103 3992 5621 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 636 420 275 217 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3103 1537 Size: 275 1291 4090 Fragments arranged by size: 4090 1291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2037 2489 2774 3586 1353 Size: 684 452 285 812 3423 Fragments arranged by size: 3423 812 684 452 285 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2273 4243 4617 4763 387 Size: 1064 138 684 1970 374 146 1280 Fragments arranged by size: 1970 1280 1064 684 374 146 138 EcoICRI GAG'CTC Cuts at: 2056 2383 2056 Size: 327 5329 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2061 2090 2576 2588 2640 2669 3719 Size: 393 429 29 486 12 52 29 1050 Cuts at: 3719 4316 5103 5371 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1050 787 597 486 429 393 268 52 29 29 12 EcoRI G'AATT_C Cuts at: 2965 3343 2965 Size: 378 5278 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2791 2791 Size: 5656 FspI TGC'GCA Cuts at: 4162 4162 Size: 5656 HaeII r_GCGC'y Cuts at: 1678 4803 1678 Size: 3125 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2381 2413 2644 2676 4297 Size: 32 135 32 809 32 231 32 1621 Cuts at: 4297 4329 4371 4403 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1621 809 231 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3000 3246 669 Size: 988 281 1062 246 3079 Fragments arranged by size: 3079 1062 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5656 HpaI GTT'AAC Cuts at: 3246 3246 Size: 5656 KpnI G_GTAC'C Cuts at: 2039 2981 2039 Size: 942 4714 MfeI C'AATT_G Cuts at: 3255 3255 Size: 5656 MluI A'CGCG_T Cuts at: 5532 5532 Size: 5656 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4770 4954 1305 Size: 3465 184 2007 Fragments arranged by size: 3465 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3834 Fragments arranged by size: 3834 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2814 3614 3973 4132 518 Size: 648 1648 800 359 159 2042 Fragments arranged by size: 2042 1648 800 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2122 2283 2415 3072 3749 4897 5142 1606 Size: 516 161 132 657 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 657 516 245 161 132 NcoI C'CATG_G Cuts at: 513 1065 2092 2735 513 Size: 552 1027 643 3434 Fragments arranged by size: 3434 1027 643 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5656 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5656 NotI GC'GGCC_GC Cuts at: 3103 3103 Size: 5656 NspI r_CATG'y Cuts at: 1132 1229 2683 3014 4559 1132 Size: 97 1454 331 1545 2229 Fragments arranged by size: 2229 1545 1454 331 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5656 PciI A'CATG_T Cuts at: 4555 4555 Size: 5656 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5656 PfoI T'CCnGG_A Cuts at: 2553 2685 2553 Size: 132 5524 PmeI GTTT'AAAC Cuts at: 2960 2960 Size: 5656 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2040 2072 3725 3757 5238 5270 2040 Size: 32 1653 32 1481 32 2426 Fragments arranged by size: 2426 1653 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5656 PsiI TTA'TAA Cuts at: 3226 3226 Size: 5656 PstI C_TGCA'G Cuts at: 830 2205 2373 3008 830 Size: 1375 168 635 3478 Fragments arranged by size: 3478 1375 635 168 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5656 PvuII CAG'CTG Cuts at: 2122 2122 Size: 5656 SacI G_AGCT'C Cuts at: 2058 2385 2058 Size: 327 5329 SalI G'TCGA_C Cuts at: 1936 2998 1936 Size: 1062 4594 SapI GCTCTTCn'nnn_ Cuts at: 2489 2774 2489 Size: 285 5371 SbfI CC_TGCA'GG Cuts at: 3008 3008 Size: 5656 ScaI AGT'ACT Cuts at: 3904 5616 3904 Size: 1712 3944 SfcI C'TryA_G Cuts at: 826 1045 2201 2369 3004 4139 4820 5011 826 Size: 219 1156 168 635 1135 681 191 1471 Fragments arranged by size: 1471 1156 1135 681 635 219 191 168 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5656 SmaI CCC'GGG Cuts at: 1872 2180 2983 1872 Size: 308 803 4545 Fragments arranged by size: 4545 803 308 SmlI C'TyrA_G Cuts at: 820 1017 1943 2792 3760 4661 4923 820 Size: 197 926 849 968 901 262 1553 Fragments arranged by size: 1553 968 926 901 849 262 197 SnaBI TAC'GTA Cuts at: 493 3447 493 Size: 2954 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5656 SphI G_CATG'C Cuts at: 1132 3014 1132 Size: 1882 3774 SspI AAT'ATT Cuts at: 5 52 3580 5 Size: 47 3528 2081 Fragments arranged by size: 3528 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5656 StyI C'CwwG_G Cuts at: 513 1065 2085 2092 2148 2735 3095 513 Size: 552 1020 7 56 587 360 3074 Fragments arranged by size: 3074 1020 587 552 360 56 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3683 3700 3853 4038 4483 325 Size: 1255 2103 17 153 185 445 1498 Fragments arranged by size: 2103 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3902 5614 96 Size: 274 80 33 51 1443 1925 1712 138 Fragments arranged by size: 1925 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5616 5648 5616 Size: 32 5624 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5656 XbaI T'CTAG_A Cuts at: 2992 2992 Size: 5656 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5656 XmaI C'CCGG_G Cuts at: 1870 2178 2981 1870 Size: 308 803 4545 Fragments arranged by size: 4545 803 308 XmnI GAAnn'nnTTC Cuts at: 3785 3785 Size: 5656 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3464 276 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI