################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsgI BsiEI BsiHKAI BsiWI BsmFI BsmI BsoBI Bsp1407I BspDI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MscI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccIII 554 TCCGGA T^CCGGA AcsI 31 GAATTT [GA]^AATT[CT] AfaI 216 GTAC GT^AC AluI 299 AGCT AG^CT AlwNI 230 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 508 GGGCCC GGGCC^C ApoI 31 GAATTT [GA]^AATT[CT] Asp700I 31 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 66 GGTCC G^G[AT]CC BanII 508 GGGCCC G[GA]GC[CT]^C BbvI 892 GCAGC GCAGC(8/12) BciVI 425 GTATCC GTATCC(6/5) BmyI 508 GGGCCC G[AGT]GC[ACT]^C BsaAI 848 TACGTA [CT]AC^GT[GA] BsaJI 20 CCAAGG C^C[ACGT][ACGT]GG BsaWI 554 TCCGGA [AT]^CCGG[AT] BseAI 554 TCCGGA T^CCGGA BseRI 583 GAGGAG GAGGAG(10/8) BsmAI 416 GTCTC GTCTC(1/5) BsmBI 415 CGTCTC CGTCTC(1/5) Bsp1286I 508 GGGCCC G[AGT]GC[ACT]^C BspEI 554 TCCGGA T^CCGGA BspHI 404 TCATGA T^CATGA BspMI 721 ACCTGC ACCTGC(4/8) BsrDI 291 CATTGC GCAATG(2/0) BsrI 43 ACTGG ACTGG(1/-1) BstEII 909 GGTCACC G^GT[ACGT]ACC BstPI 909 GGTCACC G^GT[ACGT]ACC Cac8I 781 GCTCGC GC[ACGT]^[ACGT]GC DdeI 690 CTCAG C^T[ACGT]AG EcoO65I 909 GGTCACC G^GT[ACGT]ACC EcoT14I 20 CCAAGG C^C[AT][AT]GG EcoT22I 288 ATGCAT ATGCA^T Fnu4HI 892 GCAGC GC^[ACGT]GC HapII 555 CCGG C^CGG HpaII 555 CCGG C^CGG ItaI 892 GCAGC GC^[ACGT]GC MroI 554 TCCGGA T^CCGGA MspI 555 CCGG C^CGG MwoI 190 GCTAATCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 508 GGGCCC GG[ACGT]^[ACGT]CC NsiI 288 ATGCAT ATGCA^T RcaI 404 TCATGA T^CATGA RsaI 216 GTAC GT^AC SapI 300 GCTCTTC GCTCTTC(1/4) SfaNI 287 GATGC GCATC(5/9) SfcI 600 CTGTAG C^T[GA][CT]AG SnaBI 848 TACGTA TAC^GTA SpeI 72 ACTAGT A^CTAGT SspI 837 AATATT AAT^ATT StyI 20 CCAAGG C^C[AT][AT]GG TaqI 492 TCGA T^CGA TfiI 408 GAATC G^A[AT]TC TseI 892 GCAGC G^C[AT]GC TthHB8I 492 TCGA T^CGA XbaI 150 TCTAGA T^CTAGA XcmI 896 CCATGAGGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 31 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BpmI 59 CTCCAG CTGGAG(16/14) 444 CTGGAG BsiYI 443 CCTGGAGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 499 CCTTTCTGTGG BslI 443 CCTGGAGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 499 CCTTTCTGTGG DpnII 597 GATC ^GATC 615 GATC EarI 219 CTCTTC CTCTTC(1/4) 301 CTCTTC Ksp632I 219 CTCTTC CTCTTC(1/4) 301 CTCTTC MaeII 414 ACGT A^CGT 849 ACGT MboI 597 GATC ^GATC 615 GATC MseI 643 TTAA T^TAA 835 TTAA NdeII 597 GATC ^GATC 615 GATC PleI 57 GACTC GAGTC(4/5) 793 GACTC Sau3AI 597 GATC ^GATC 615 GATC Tru9I 643 TTAA T^TAA 835 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### BfaI BssKI BstF5I BstNI Cfr13I EcoRII FokI HaeIII HinfI HphI MaeI MaeIII MboII MnlI MslI MvaI NlaIII Sau96I ScrFI Tsp45I Tsp509I TspRI