(Linear) (Six-base) MAPSORT of: KIEE2358.seq Check: 4449 from: 1 to: 1263 KIEE2358.orf With 182 enzymes: SgfI * July 7, 2011 16:40 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 754 1263 Size: 754 509 AfeI AGC'GCT Cuts at: 0 379 1263 Size: 379 884 AflIII A'CryG_T Cuts at: 0 485 1263 Size: 485 778 AgeI A'CCGG_T Cuts at: 0 51 1263 Size: 51 1212 AleI CACnn'nnGTG Cuts at: 0 1229 1263 Size: 1229 34 AlwNI CAG_nnn'CTG Cuts at: 0 460 1263 Size: 460 803 ApaI G_GGCC'C Cuts at: 0 467 1263 Size: 467 796 ApoI r'AATT_y Cuts at: 0 689 1263 Size: 689 574 AvaI C'yCGr_G Cuts at: 0 256 346 394 1263 Size: 256 90 48 869 Fragments arranged by size: 869 256 90 48 BanII G_rGCy'C Cuts at: 0 26 230 257 467 710 1163 1170 1263 Size: 26 204 27 210 243 453 7 93 Fragments arranged by size: 453 243 210 204 93 27 26 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 587 1263 Size: 587 676 BglI GCCn_nnn'nGGC Cuts at: 0 234 1263 Size: 234 1029 Bme1580I G_kGCm'C Cuts at: 0 467 1079 1263 Size: 467 612 184 Fragments arranged by size: 612 467 184 BmgBI CAC'GTC Cuts at: 0 291 1263 Size: 291 972 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 457 1263 Size: 457 806 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 40 205 337 518 532 634 1189 1263 Size: 40 165 132 181 14 102 555 74 Fragments arranged by size: 555 181 165 132 102 74 40 14 Bpu10I CC'TnA_GC Cuts at: 0 1181 1263 Size: 1181 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 1263 Size: 244 1019 BsaI GGTCTCn'nnnn_ Cuts at: 0 802 1263 Size: 802 461 BsaHI Gr'CG_yC Cuts at: 0 827 1263 Size: 827 436 BsaWI w'CCGG_w Cuts at: 0 51 1263 Size: 51 1212 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1053 1263 Size: 1053 210 BseYI C'CCAG_C Cuts at: 0 41 201 535 1263 Size: 41 160 334 728 Fragments arranged by size: 728 334 160 41 BsiHKAI G_wGCw'C Cuts at: 0 710 949 1263 Size: 710 239 314 Fragments arranged by size: 710 314 239 BsmBI CGTCTCn'nnnn_ Cuts at: 0 595 1263 Size: 595 668 Bsp1286I G_dGCh'C Cuts at: 0 26 230 257 467 710 949 1079 1163 Size: 26 204 27 210 243 239 130 84 Cuts at: 1163 1170 1263 Size: 7 93 Fragments arranged by size: 243 239 210 204 130 93 84 27 26 7 BsrFI r'CCGG_y Cuts at: 0 51 90 411 1263 Size: 51 39 321 852 Fragments arranged by size: 852 321 51 39 BssHII G'CGCG_C Cuts at: 0 102 128 148 189 191 417 1263 Size: 102 26 20 41 2 226 846 Fragments arranged by size: 846 226 102 41 26 20 2 BssSI C'ACGA_G Cuts at: 0 373 1237 1263 Size: 373 864 26 Fragments arranged by size: 864 373 26 BstEII G'GTnAC_C Cuts at: 0 849 1263 Size: 849 414 BstXI CCAn_nnnn'nTGG Cuts at: 0 597 1263 Size: 597 666 Bsu36I CC'TnA_GG Cuts at: 0 656 1263 Size: 656 607 BtgI C'CryG_G Cuts at: 0 26 230 308 596 719 1032 1175 1263 Size: 26 204 78 288 123 313 143 88 Fragments arranged by size: 313 288 204 143 123 88 78 26 EaeI y'GGCC_r Cuts at: 0 305 330 773 1233 1263 Size: 305 25 443 460 30 Fragments arranged by size: 460 443 305 30 25 EarI CTCTTCn'nnn_ Cuts at: 0 448 910 982 1263 Size: 448 462 72 281 Fragments arranged by size: 462 448 281 72 EcoICRI GAG'CTC Cuts at: 0 708 1263 Size: 708 555 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 40 205 337 518 532 634 754 1189 Size: 40 165 132 181 14 102 120 435 Cuts at: 1189 1263 Size: 74 Fragments arranged by size: 435 181 165 132 120 102 74 40 14 EcoNI CCTnn'n_nnAGG Cuts at: 0 899 1263 Size: 899 364 EcoO109I rG'GnC_Cy Cuts at: 0 464 904 1263 Size: 464 440 359 Fragments arranged by size: 464 440 359 HaeII r_GCGC'y Cuts at: 0 381 1263 Size: 381 882 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 594 626 1263 Size: 594 32 637 Fragments arranged by size: 637 594 32 HincII GTy'rAC Cuts at: 0 831 1263 Size: 831 432 MscI TGG'CCA Cuts at: 0 332 775 1235 1263 Size: 332 443 460 28 Fragments arranged by size: 460 443 332 28 MslI CAynn'nnrTG Cuts at: 0 847 928 1229 1263 Size: 847 81 301 34 Fragments arranged by size: 847 301 81 34 MspA1I CmG'CkG Cuts at: 0 28 35 1263 Size: 28 7 1228 Fragments arranged by size: 1228 28 7 NcoI C'CATG_G Cuts at: 0 230 596 719 1032 1175 1263 Size: 230 366 123 313 143 88 Fragments arranged by size: 366 313 230 143 123 88 NspI r_CATG'y Cuts at: 0 489 1126 1263 Size: 489 637 137 Fragments arranged by size: 637 489 137 PasI CC'CwG_GG Cuts at: 0 1163 1263 Size: 1163 100 PciI A'CATG_T Cuts at: 0 485 1263 Size: 485 778 PflMI CCAn_nnn'nTGG Cuts at: 0 460 676 1263 Size: 460 216 587 Fragments arranged by size: 587 460 216 PfoI T'CCnGG_A Cuts at: 0 17 669 1263 Size: 17 652 594 Fragments arranged by size: 652 594 17 PpuMI rG'GwC_Cy Cuts at: 0 904 1263 Size: 904 359 PspOMI G'GGCC_C Cuts at: 0 463 1263 Size: 463 800 SacI G_AGCT'C Cuts at: 0 710 1263 Size: 710 553 SacII CC_GC'GG Cuts at: 0 29 1263 Size: 29 1234 SapI GCTCTTCn'nnn_ Cuts at: 0 448 910 1263 Size: 448 462 353 Fragments arranged by size: 462 448 353 ScaI AGT'ACT Cuts at: 0 619 1263 Size: 619 644 SmaI CCC'GGG Cuts at: 0 348 396 1263 Size: 348 48 867 Fragments arranged by size: 867 348 48 SmlI C'TyrA_G Cuts at: 0 223 1263 Size: 223 1040 SphI G_CATG'C Cuts at: 0 1126 1263 Size: 1126 137 StuI AGG'CCT Cuts at: 0 1012 1263 Size: 1012 251 StyI C'CwwG_G Cuts at: 0 168 230 248 266 302 596 719 1032 Size: 168 62 18 18 36 294 123 313 Cuts at: 1032 1127 1175 1263 Size: 95 48 88 Fragments arranged by size: 313 294 168 123 95 88 62 48 36 18 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1219 1263 Size: 1219 44 TatI w'GTAC_w Cuts at: 0 617 1263 Size: 617 646 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1182 1263 Size: 1182 81 XmaI C'CCGG_G Cuts at: 0 346 394 1263 Size: 346 48 869 Fragments arranged by size: 869 346 48 XmnI GAAnn'nnTTC Cuts at: 0 919 1263 Size: 919 344 Enzymes that do cut: AcuI AfeI AflIII AgeI AleI AlwNI ApaI ApoI AvaI BanII BbsI BglI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI BsiHKAI BsmBI Bsp1286I BsrFI BssHII BssSI BstEII BstXI Bsu36I BtgI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MscI MslI MspA1I NcoI NspI PasI PciI PflMI PfoI PpuMI PspOMI SacI SacII SapI ScaI SmaI SmlI SphI StuI StyI TaqII TatI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmrI BmtI BsaAI BsaBI BsaXI BsgI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI BstAPI BstBI BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TstI Tth111I XbaI XhoI ZraI