(Linear) (Six-base) MAPSORT of: KIEE4204.seq Check: 8860 from: 1 to: 2133 KIEE4204.orf With 182 enzymes: SgfI * March 22, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 506 705 1647 2133 Size: 506 199 942 486 Fragments arranged by size: 942 506 486 199 AccI GT'mk_AC Cuts at: 0 596 1388 2133 Size: 596 792 745 Fragments arranged by size: 792 745 596 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 131 892 1499 1743 2133 Size: 131 761 607 244 390 Fragments arranged by size: 761 607 390 244 131 AgeI A'CCGG_T Cuts at: 0 2006 2133 Size: 2006 127 AleI CACnn'nnGTG Cuts at: 0 1015 2133 Size: 1015 1118 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 961 2133 Size: 961 1172 AlwNI CAG_nnn'CTG Cuts at: 0 745 1291 2133 Size: 745 546 842 Fragments arranged by size: 842 745 546 ApaLI G'TGCA_C Cuts at: 0 565 2133 Size: 565 1568 BamHI G'GATC_C Cuts at: 0 188 1887 2133 Size: 188 1699 246 Fragments arranged by size: 1699 246 188 BanI G'GyrC_C Cuts at: 0 1002 1946 2133 Size: 1002 944 187 Fragments arranged by size: 1002 944 187 BbsI GAAGACnn'nnnn_ Cuts at: 0 1430 1505 2133 Size: 1430 75 628 Fragments arranged by size: 1430 628 75 BbvCI CC'TCA_GC Cuts at: 0 1712 2133 Size: 1712 421 BclI T'GATC_A Cuts at: 0 1341 2133 Size: 1341 792 BglI GCCn_nnn'nGGC Cuts at: 0 1980 2133 Size: 1980 153 BlpI GC'TnA_GC Cuts at: 0 1583 2133 Size: 1583 550 Bme1580I G_kGCm'C Cuts at: 0 569 2133 Size: 569 1564 BmrI ACTGGGnnnn_n' Cuts at: 0 1423 1479 2067 2133 Size: 1423 56 588 66 Fragments arranged by size: 1423 588 66 56 BmtI G_CTAG'C Cuts at: 0 122 667 671 2133 Size: 122 545 4 1462 Fragments arranged by size: 1462 545 122 4 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 10 1696 2133 Size: 10 1686 437 Fragments arranged by size: 1686 437 10 Bpu10I CC'TnA_GC Cuts at: 0 530 1712 2133 Size: 530 1182 421 Fragments arranged by size: 1182 530 421 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 820 891 1654 2133 Size: 820 71 763 479 Fragments arranged by size: 820 763 479 71 BsaI GGTCTCn'nnnn_ Cuts at: 0 1672 2015 2133 Size: 1672 343 118 Fragments arranged by size: 1672 343 118 BsaHI Gr'CG_yC Cuts at: 0 992 2133 Size: 992 1141 BsaWI w'CCGG_w Cuts at: 0 2006 2133 Size: 2006 127 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 850 880 1669 1699 2133 Size: 850 30 789 30 434 Fragments arranged by size: 850 789 434 30 30 BseYI C'CCAG_C Cuts at: 0 601 1543 1859 2037 2133 Size: 601 942 316 178 96 Fragments arranged by size: 942 601 316 178 96 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 231 669 1178 2133 Size: 231 438 509 955 Fragments arranged by size: 955 509 438 231 BsiHKAI G_wGCw'C Cuts at: 0 41 557 569 660 2133 Size: 41 516 12 91 1473 Fragments arranged by size: 1473 516 91 41 12 BsmI GAATG_Cn' Cuts at: 0 273 361 2133 Size: 273 88 1772 Fragments arranged by size: 1772 273 88 Bsp1286I G_dGCh'C Cuts at: 0 41 557 569 660 2133 Size: 41 516 12 91 1473 Fragments arranged by size: 1473 516 91 41 12 BspHI T'CATG_A Cuts at: 0 2046 2133 Size: 2046 87 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 506 705 1647 1810 2133 Size: 506 199 942 163 323 Fragments arranged by size: 942 506 323 199 163 BsrDI GCAATG_nn' Cuts at: 0 1123 1969 2133 Size: 1123 846 164 Fragments arranged by size: 1123 846 164 BsrFI r'CCGG_y Cuts at: 0 2006 2133 Size: 2006 127 BstAPI GCAn_nnn'nTGC Cuts at: 0 277 367 1901 2133 Size: 277 90 1534 232 Fragments arranged by size: 1534 277 232 90 BstEII G'GTnAC_C Cuts at: 0 1238 2133 Size: 1238 895 BstXI CCAn_nnnn'nTGG Cuts at: 0 1991 2133 Size: 1991 142 BstYI r'GATC_y Cuts at: 0 188 397 465 1869 1887 2133 Size: 188 209 68 1404 18 246 Fragments arranged by size: 1404 246 209 188 68 18 Bsu36I CC'TnA_GG Cuts at: 0 1079 2133 Size: 1079 1054 BtsI GCAGTG_nn' Cuts at: 0 746 2133 Size: 746 1387 DraI TTT'AAA Cuts at: 0 1412 1689 2133 Size: 1412 277 444 Fragments arranged by size: 1412 444 277 DraIII CAC_nnn'GTG Cuts at: 0 573 2133 Size: 573 1560 EaeI y'GGCC_r Cuts at: 0 717 2133 Size: 717 1416 EarI CTCTTCn'nnn_ Cuts at: 0 220 1611 2117 2133 Size: 220 1391 506 16 Fragments arranged by size: 1391 506 220 16 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 10 131 892 1499 1696 1743 2133 Size: 10 121 761 607 197 47 390 Fragments arranged by size: 761 607 390 197 121 47 10 EcoRV GAT'ATC Cuts at: 0 318 2133 Size: 318 1815 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 328 2133 Size: 328 1805 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 180 212 1303 1335 1543 1575 2133 Size: 180 32 1091 32 208 32 558 Fragments arranged by size: 1091 558 208 180 32 32 32 HincII GTy'rAC Cuts at: 0 510 2133 Size: 510 1623 HpaI GTT'AAC Cuts at: 0 510 2133 Size: 510 1623 MfeI C'AATT_G Cuts at: 0 701 2133 Size: 701 1432 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 836 1627 2133 Size: 836 791 506 Fragments arranged by size: 836 791 506 MscI TGG'CCA Cuts at: 0 719 2133 Size: 719 1414 MslI CAynn'nnrTG Cuts at: 0 1015 2133 Size: 1015 1118 NheI G'CTAG_C Cuts at: 0 118 663 667 2133 Size: 118 545 4 1466 Fragments arranged by size: 1466 545 118 4 NspI r_CATG'y Cuts at: 0 113 545 2133 Size: 113 432 1588 Fragments arranged by size: 1588 432 113 PflMI CCAn_nnn'nTGG Cuts at: 0 1746 2133 Size: 1746 387 PfoI T'CCnGG_A Cuts at: 0 1673 2133 Size: 1673 460 PstI C_TGCA'G Cuts at: 0 741 2133 Size: 741 1392 ScaI AGT'ACT Cuts at: 0 1831 2133 Size: 1831 302 SexAI A'CCwGG_T Cuts at: 0 1458 1681 2133 Size: 1458 223 452 Fragments arranged by size: 1458 452 223 SfcI C'TryA_G Cuts at: 0 233 737 1878 2133 Size: 233 504 1141 255 Fragments arranged by size: 1141 504 255 233 SmlI C'TyrA_G Cuts at: 0 799 906 1633 2133 Size: 799 107 727 500 Fragments arranged by size: 799 727 500 107 StuI AGG'CCT Cuts at: 0 609 1093 2133 Size: 609 484 1040 Fragments arranged by size: 1040 609 484 StyI C'CwwG_G Cuts at: 0 347 479 1382 2133 Size: 347 132 903 751 Fragments arranged by size: 903 751 347 132 TatI w'GTAC_w Cuts at: 0 1618 1718 1829 2133 Size: 1618 100 111 304 Fragments arranged by size: 1618 304 111 100 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 850 882 2133 Size: 850 32 1251 Fragments arranged by size: 1251 850 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 317 486 892 1782 2133 Size: 317 169 406 890 351 Fragments arranged by size: 890 406 351 317 169 XmnI GAAnn'nnTTC Cuts at: 0 2020 2133 Size: 2020 113 Enzymes that do cut: AarI AccI AcuI AgeI AleI AlfI AlwNI ApaLI BamHI BanI BbsI BbvCI BclI BglI BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtsI DraI DraIII EaeI EarI Eco57MI EcoRV FalI Hin4I HincII HpaI MfeI MmeI MscI MslI NheI NspI PflMI PfoI PstI ScaI SexAI SfcI SmlI StuI StyI TatI TstI XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AhdI AloI ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI BanII BbeI BcgI BciVI BfrBI BglII BmgBI BplI BsaAI BsaBI BseRI BsiEI BsiWI BsmBI BspEI BsrBI BsrGI BssHII BssSI BstBI BstZ17I BtgI BtgZI ClaI CspCI DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII HindIII KasI KpnI MluI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI