(Circular) (Six-base) MAPSORT of: pFN21AE4281.seq Check: 4702 from: 1 to: 9211 pFN21AE4281 9211 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7021 278 Size: 53 83 186 936 5485 2468 Fragments arranged by size: 5485 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6290 6554 1937 Size: 4353 264 4594 Fragments arranged by size: 4594 4353 264 Acc65I G'GTAC_C Cuts at: 6532 6532 Size: 9211 AclI AA'CG_TT Cuts at: 1563 7338 7711 1563 Size: 5775 373 3063 Fragments arranged by size: 5775 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2475 4065 4731 5250 5271 6425 7274 8658 Size: 843 1590 666 519 21 1154 849 1384 Cuts at: 8658 8926 1632 Size: 268 1917 Fragments arranged by size: 1917 1590 1384 1154 849 843 666 519 268 21 AflII C'TTAA_G Cuts at: 820 1017 3632 3754 820 Size: 197 2615 122 6277 Fragments arranged by size: 6277 2615 197 122 AflIII A'CryG_T Cuts at: 1203 2714 3671 5756 8110 9087 1203 Size: 1511 957 2085 2354 977 1327 Fragments arranged by size: 2354 2085 1511 1327 977 957 AgeI A'CCGG_T Cuts at: 8042 8042 Size: 9211 AhdI GACnn_n'nnGTC Cuts at: 7940 7940 Size: 9211 AleI CACnn'nnGTG Cuts at: 1166 3973 1166 Size: 2807 6404 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3735 1184 Size: 2551 6660 AlwNI CAG_nnn'CTG Cuts at: 3265 4331 4658 4917 5803 6954 8526 3265 Size: 1066 327 259 886 1151 1572 3950 Fragments arranged by size: 3950 1572 1151 1066 886 327 259 ApaLI G'TGCA_C Cuts at: 7268 8424 7268 Size: 1156 8055 ApoI r'AATT_y Cuts at: 1493 2271 2335 2537 3302 3324 3555 3888 4022 Size: 778 64 202 765 22 231 333 134 Cuts at: 4022 4223 4745 4843 5364 5852 6131 6520 6746 Size: 201 522 98 521 488 279 389 226 Cuts at: 6746 6898 9007 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 778 765 522 521 488 389 333 279 231 226 202 201 152 134 98 64 22 AseI AT'TA_AT Cuts at: 160 1032 3189 4097 7765 160 Size: 872 2157 908 3668 1606 Fragments arranged by size: 3668 2157 1606 908 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9211 AvaI C'yCGr_G Cuts at: 1870 1943 5229 6536 1870 Size: 73 3286 1307 4545 Fragments arranged by size: 4545 3286 1307 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2723 2756 3757 3790 2723 Size: 33 1001 33 8144 Fragments arranged by size: 8144 1001 33 33 BamHI G'GATC_C Cuts at: 5260 6493 6541 6908 5260 Size: 1233 48 367 7563 Fragments arranged by size: 7563 1233 367 48 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4149 5233 6433 6532 7987 Size: 325 217 627 2362 1084 1200 99 1455 Cuts at: 7987 618 Size: 1842 Fragments arranged by size: 2362 1842 1455 1200 1084 627 325 217 99 BanII G_rGCy'C Cuts at: 1391 4301 6004 6530 1391 Size: 2910 1703 526 4072 Fragments arranged by size: 4072 2910 1703 526 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5581 6095 928 Size: 971 3682 514 4044 Fragments arranged by size: 4044 3682 971 514 BbvCI CC'TCA_GC Cuts at: 6465 6465 Size: 9211 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4579 4613 7402 7436 1903 Size: 34 2642 34 2789 34 3678 Fragments arranged by size: 3678 2789 2642 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5070 7103 8313 5070 Size: 2033 1210 5968 Fragments arranged by size: 5968 2033 1210 BclI T'GATC_A Cuts at: 1548 2212 2410 2527 5941 1548 Size: 664 198 117 3414 4818 Fragments arranged by size: 4818 3414 664 198 117 BfrBI ATG'CAT Cuts at: 2490 2490 Size: 9211 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 7822 136 Size: 107 122 71 631 605 337 5813 1525 Fragments arranged by size: 5813 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3517 6022 9206 3517 Size: 2505 3184 3522 Fragments arranged by size: 3522 3184 2505 BlpI GC'TnA_GC Cuts at: 2174 6628 2174 Size: 4454 4757 Bme1580I G_kGCm'C Cuts at: 1790 7272 8428 1790 Size: 5482 1156 2573 Fragments arranged by size: 5482 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9211 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2236 6942 7900 Size: 416 53 83 399 24 819 4706 958 Cuts at: 7900 442 Size: 1753 Fragments arranged by size: 4706 1753 958 819 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9211 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5780 6018 7871 1239 Size: 4541 238 1853 2579 Fragments arranged by size: 4541 2579 1853 238 Bpu10I CC'TnA_GC Cuts at: 3218 6465 3218 Size: 3247 5964 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4357 4413 7336 8201 8499 4357 Size: 56 2923 865 298 5069 Fragments arranged by size: 5069 2923 865 298 56 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6253 7874 882 Size: 617 4754 1621 2219 Fragments arranged by size: 4754 2219 1621 617 BsaAI yAC'GTr Cuts at: 493 1204 5759 6166 7002 493 Size: 711 4555 407 836 2702 Fragments arranged by size: 4555 2702 836 711 407 BsaBI GATnn'nnATC Cuts at: 2625 3516 5259 5946 9205 2625 Size: 891 1743 687 3259 2631 Fragments arranged by size: 3259 2631 1743 891 687 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7018 7400 8035 275 Size: 53 83 186 936 5485 382 635 1451 Fragments arranged by size: 5485 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7644 8042 8316 8463 8874 7644 Size: 398 274 147 411 7981 Fragments arranged by size: 7981 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2184 2214 6351 6381 1178 Size: 30 165 30 781 30 4137 30 4008 Fragments arranged by size: 4137 4008 781 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3295 4272 6381 1186 Size: 2109 977 2109 4016 Fragments arranged by size: 4016 2109 2109 977 BseYI C'CCAG_C Cuts at: 8414 8910 8414 Size: 496 8715 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2379 2709 2864 1163 Size: 573 643 330 155 7510 Fragments arranged by size: 7510 643 573 330 155 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3911 6661 7422 8450 1540 Size: 275 186 1910 2750 761 1028 2301 Fragments arranged by size: 2750 2301 1910 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3224 7272 7357 8428 3224 Size: 4048 85 1071 4007 Fragments arranged by size: 4048 4007 1071 85 BsmI GAATG_Cn' Cuts at: 2270 2618 3493 4602 6722 6815 2270 Size: 348 875 1109 2120 93 4666 Fragments arranged by size: 4666 2120 1109 875 348 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3224 4301 6004 6530 7272 7357 8428 Size: 399 1434 1077 1703 526 742 85 1071 Cuts at: 8428 1391 Size: 2174 Fragments arranged by size: 2174 1703 1434 1077 1071 742 526 399 85 BspEI T'CCGG_A Cuts at: 8874 8874 Size: 9211 BspHI T'CATG_A Cuts at: 5086 7100 8830 5086 Size: 2014 1730 5467 Fragments arranged by size: 5467 2014 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4210 6566 844 Size: 3366 2356 3489 Fragments arranged by size: 3489 3366 2356 BsrBI CCG'CTC Cuts at: 1394 1823 7098 1394 Size: 429 5275 3507 Fragments arranged by size: 5275 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4187 4722 6455 7706 7880 65 Size: 1183 2939 535 1733 1251 174 1396 Fragments arranged by size: 2939 1733 1396 1251 1183 535 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3910 7855 8042 1700 Size: 110 2100 3945 187 2869 Fragments arranged by size: 3945 2869 2100 187 110 BsrGI T'GTAC_A Cuts at: 96 2397 3256 3692 4639 4996 96 Size: 2301 859 436 947 357 4311 Fragments arranged by size: 4311 2301 947 859 436 357 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9211 BssSI C'ACGA_G Cuts at: 7271 8283 7271 Size: 1012 8199 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9211 BstBI TT'CG_AA Cuts at: 5368 8015 5368 Size: 2647 6564 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9211 BstYI r'GATC_y Cuts at: 1972 3517 4366 4381 5260 5596 5800 6022 6031 Size: 1545 849 15 879 336 204 222 9 Cuts at: 6031 6493 6541 6908 7293 7310 8762 8848 8860 Size: 462 48 367 385 17 1452 86 12 Cuts at: 8860 9206 1972 Size: 346 1977 Fragments arranged by size: 1977 1545 1452 879 849 462 385 367 346 336 222 204 86 48 17 15 12 9 BtgI C'CryG_G Cuts at: 513 1065 3350 513 Size: 552 2285 6374 Fragments arranged by size: 6374 2285 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8472 Fragments arranged by size: 8472 683 56 BtsI GCAGTG_nn' Cuts at: 4426 4737 6341 6727 7521 7541 4426 Size: 311 1604 386 794 20 6096 Fragments arranged by size: 6096 1604 794 386 311 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9211 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 6366 6401 6426 6461 529 Size: 35 5802 35 25 35 3279 Fragments arranged by size: 5802 3279 35 35 35 25 DraI TTT'AAA Cuts at: 3964 5181 6515 3964 Size: 1217 1334 6660 Fragments arranged by size: 6660 1334 1217 DraIII CAC_nnn'GTG Cuts at: 5018 5704 5018 Size: 686 8525 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8218 809 Size: 507 6902 1802 Fragments arranged by size: 6902 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3682 3748 3908 Size: 54 1425 50 275 18 1852 66 160 Cuts at: 3908 6245 6658 7547 9176 8 Size: 2337 413 889 1629 43 Fragments arranged by size: 2337 1852 1629 1425 889 413 275 160 66 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3908 6658 1537 Size: 275 2096 2750 4090 Fragments arranged by size: 4090 2750 2096 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3006 3060 3272 3462 4522 6057 7141 1353 Size: 1653 54 212 190 1060 1535 1084 3423 Fragments arranged by size: 3423 1653 1535 1084 1060 212 190 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3214 7798 8172 8318 387 Size: 1064 138 1625 4584 374 146 1280 Fragments arranged by size: 4584 1625 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2475 4065 4731 5250 5271 5780 6018 Size: 393 843 1590 666 519 21 509 238 Cuts at: 6018 6425 7274 7871 8658 8926 1239 Size: 407 849 597 787 268 1524 Fragments arranged by size: 1590 1524 849 843 787 666 597 519 509 407 393 268 238 21 EcoRI G'AATT_C Cuts at: 6520 6898 6520 Size: 378 8833 EcoRV GAT'ATC Cuts at: 3270 3270 Size: 9211 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3500 3812 5618 3500 Size: 312 1806 7093 Fragments arranged by size: 7093 1806 312 FspI TGC'GCA Cuts at: 7717 7717 Size: 9211 HaeII r_GCGC'y Cuts at: 1678 8358 1678 Size: 6680 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2685 2717 2845 2877 3605 Size: 32 135 32 1113 32 128 32 728 Cuts at: 3605 3637 3908 3940 5411 5443 6160 6192 6215 Size: 32 271 32 1471 32 717 32 23 Cuts at: 6215 6247 6272 6304 7852 7884 7926 7958 1373 Size: 32 25 32 1548 32 42 32 2626 Fragments arranged by size: 2626 1548 1471 1113 728 717 271 135 128 42 32 32 32 32 32 32 32 32 32 32 32 32 25 23 HincII GTy'rAC Cuts at: 669 1657 1938 2704 3793 6555 6801 669 Size: 988 281 766 1089 2762 246 3079 Fragments arranged by size: 3079 2762 1089 988 766 281 246 HindIII A'AGCT_T Cuts at: 748 3093 3591 4047 4584 6009 748 Size: 2345 498 456 537 1425 3950 Fragments arranged by size: 3950 2345 1425 537 498 456 HpaI GTT'AAC Cuts at: 3793 6801 3793 Size: 3008 6203 KpnI G_GTAC'C Cuts at: 6536 6536 Size: 9211 MfeI C'AATT_G Cuts at: 4521 4983 5187 6810 4521 Size: 462 204 1623 6922 Fragments arranged by size: 6922 1623 462 204 MluI A'CGCG_T Cuts at: 9087 9087 Size: 9211 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2038 2080 2721 3720 5033 8325 8509 1305 Size: 733 42 641 999 1313 3292 184 2007 Fragments arranged by size: 3292 2007 1313 999 733 641 184 42 MscI TGG'CCA Cuts at: 10 64 1489 1832 3684 6247 10 Size: 54 1425 343 1852 2563 2974 Fragments arranged by size: 2974 2563 1852 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3704 3973 4884 5091 5523 7169 7528 Size: 648 2538 269 911 207 432 1646 359 Cuts at: 7528 7687 518 Size: 159 2042 Fragments arranged by size: 2538 2042 1646 911 648 432 359 269 207 159 MspA1I CmG'CkG Cuts at: 1606 2363 4645 5813 5901 6469 6627 7304 8452 Size: 757 2282 1168 88 568 158 677 1148 Cuts at: 8452 8697 1606 Size: 245 2120 Fragments arranged by size: 2282 2120 1168 1148 757 677 568 245 158 88 NcoI C'CATG_G Cuts at: 513 1065 3350 513 Size: 552 2285 6374 Fragments arranged by size: 6374 2285 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9211 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9211 NotI GC'GGCC_GC Cuts at: 6658 6658 Size: 9211 NsiI A_TGCA'T Cuts at: 2492 2492 Size: 9211 NspI r_CATG'y Cuts at: 1132 1229 2718 3675 6569 8114 1132 Size: 97 1489 957 2894 1545 2229 Fragments arranged by size: 2894 2229 1545 1489 957 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9211 PciI A'CATG_T Cuts at: 2714 3671 8110 2714 Size: 957 4439 3815 Fragments arranged by size: 4439 3815 957 PflMI CCAn_nnn'nTGG Cuts at: 1108 5803 1108 Size: 4695 4516 PfoI T'CCnGG_A Cuts at: 5796 6084 5796 Size: 288 8923 PmeI GTTT'AAAC Cuts at: 6515 6515 Size: 9211 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7280 7312 8793 8825 7280 Size: 32 1481 32 7666 Fragments arranged by size: 7666 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9211 PsiI TTA'TAA Cuts at: 2790 6781 2790 Size: 3991 5220 PstI C_TGCA'G Cuts at: 830 6563 830 Size: 5733 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9211 PvuII CAG'CTG Cuts at: 2363 4645 5813 6469 2363 Size: 2282 1168 656 5105 Fragments arranged by size: 5105 2282 1168 656 SalI G'TCGA_C Cuts at: 1936 6553 1936 Size: 4617 4594 SbfI CC_TGCA'GG Cuts at: 6563 6563 Size: 9211 ScaI AGT'ACT Cuts at: 7459 9171 7459 Size: 1712 7499 SfcI C'TryA_G Cuts at: 826 1045 5506 5991 6559 7694 8375 8566 826 Size: 219 4461 485 568 1135 681 191 1471 Fragments arranged by size: 4461 1471 1135 681 568 485 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9211 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9211 SmaI CCC'GGG Cuts at: 1872 5231 6538 1872 Size: 3359 1307 4545 Fragments arranged by size: 4545 3359 1307 SmlI C'TyrA_G Cuts at: 820 1017 1943 3632 3754 4336 4428 7315 8216 Size: 197 926 1689 122 582 92 2887 901 Cuts at: 8216 8478 820 Size: 262 1553 Fragments arranged by size: 2887 1689 1553 926 901 582 262 197 122 92 SnaBI TAC'GTA Cuts at: 493 7002 493 Size: 6509 2702 SpeI A'CTAG_T Cuts at: 152 4543 152 Size: 4391 4820 SphI G_CATG'C Cuts at: 1132 6569 1132 Size: 5437 3774 SspI AAT'ATT Cuts at: 5 52 2150 4503 5926 7135 5 Size: 47 2098 2353 1423 1209 2081 Fragments arranged by size: 2353 2098 2081 1423 1209 47 StuI AGG'CCT Cuts at: 1517 5452 1517 Size: 3935 5276 StyI C'CwwG_G Cuts at: 513 1065 3158 3350 4153 5392 6650 513 Size: 552 2093 192 803 1239 1258 3074 Fragments arranged by size: 3074 2093 1258 1239 803 552 192 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3963 5405 7238 7255 7408 7593 8038 Size: 1255 2383 1442 1833 17 153 185 445 Cuts at: 8038 325 Size: 1498 Fragments arranged by size: 2383 1833 1498 1442 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2223 2397 3256 Size: 274 80 33 51 1443 246 174 859 Cuts at: 3256 3692 4639 4996 7457 9169 96 Size: 436 947 357 2461 1712 138 Fragments arranged by size: 2461 1712 1443 947 859 436 357 274 246 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3350 3382 6405 6437 9171 9203 3350 Size: 32 3023 32 2734 32 3358 Fragments arranged by size: 3358 3023 2734 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9211 XbaI T'CTAG_A Cuts at: 3061 4819 6547 3061 Size: 1758 1728 5725 Fragments arranged by size: 5725 1758 1728 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3348 4520 3348 Size: 1172 8039 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9211 XmaI C'CCGG_G Cuts at: 1870 5229 6536 1870 Size: 3359 1307 4545 Fragments arranged by size: 4545 3359 1307 XmnI GAAnn'nnTTC Cuts at: 3324 3471 3624 4545 5518 6102 7340 3324 Size: 147 153 921 973 584 1238 5195 Fragments arranged by size: 5195 1238 973 921 584 153 147 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7019 276 Size: 53 83 186 936 5485 2468 Fragments arranged by size: 5485 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI