(Circular) (Six-base) MAPSORT of: pFN21AE5480.seq Check: 8704 from: 1 to: 5698 pFN21AE5480 5698 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3508 278 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3041 1937 Size: 1104 4594 Acc65I G'GTAC_C Cuts at: 3019 3019 Size: 5698 AclI AA'CG_TT Cuts at: 1563 3825 4198 1563 Size: 2262 373 3063 Fragments arranged by size: 3063 2262 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2691 3761 5145 5413 1632 Size: 1059 1070 1384 268 1917 Fragments arranged by size: 1917 1384 1070 1059 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5501 AflIII A'CryG_T Cuts at: 1203 2922 4597 5574 1203 Size: 1719 1675 977 1327 Fragments arranged by size: 1719 1675 1327 977 AgeI A'CCGG_T Cuts at: 4529 4529 Size: 5698 AhdI GACnn_n'nnGTC Cuts at: 4427 4427 Size: 5698 AleI CACnn'nnGTG Cuts at: 1166 2028 2657 1166 Size: 862 629 4207 Fragments arranged by size: 4207 862 629 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5698 AlwNI CAG_nnn'CTG Cuts at: 2495 3441 5013 2495 Size: 946 1572 3180 Fragments arranged by size: 3180 1572 946 ApaLI G'TGCA_C Cuts at: 3755 4911 3755 Size: 1156 4542 ApoI r'AATT_y Cuts at: 1493 3007 3233 3385 5494 1493 Size: 1514 226 152 2109 1697 Fragments arranged by size: 2109 1697 1514 226 152 AseI AT'TA_AT Cuts at: 160 1032 4252 160 Size: 872 3220 1606 Fragments arranged by size: 3220 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 AvaI C'yCGr_G Cuts at: 1870 1943 2330 3023 1870 Size: 73 387 693 4545 Fragments arranged by size: 4545 693 387 73 BamHI G'GATC_C Cuts at: 3028 3395 3028 Size: 367 5331 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3019 4474 618 Size: 325 217 627 1232 1455 1842 Fragments arranged by size: 1842 1455 1232 627 325 217 BanII G_rGCy'C Cuts at: 1391 2061 3017 1391 Size: 670 956 4072 Fragments arranged by size: 4072 956 670 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4727 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3889 3923 1903 Size: 34 1952 34 3678 Fragments arranged by size: 3678 1952 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3590 4800 3590 Size: 1210 4488 BclI T'GATC_A Cuts at: 1548 2649 1548 Size: 1101 4597 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4309 136 Size: 107 122 71 631 605 2637 1525 Fragments arranged by size: 2637 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5693 5693 Size: 5698 BlpI GC'TnA_GC Cuts at: 2255 3115 2255 Size: 860 4838 Bme1580I G_kGCm'C Cuts at: 1790 3759 4915 1790 Size: 1969 1156 2573 Fragments arranged by size: 2573 1969 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5698 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2836 3429 4387 Size: 416 53 83 399 24 1419 593 958 Cuts at: 4387 442 Size: 1753 Fragments arranged by size: 1753 1419 958 593 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5698 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2618 4358 1239 Size: 1379 1740 2579 Fragments arranged by size: 2579 1740 1379 Bpu10I CC'TnA_GC Cuts at: 2690 2690 Size: 5698 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2254 3823 4688 4986 2254 Size: 1569 865 298 2966 Fragments arranged by size: 2966 1569 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4361 882 Size: 617 2862 2219 Fragments arranged by size: 2862 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2891 3489 493 Size: 711 1687 598 2702 Fragments arranged by size: 2702 1687 711 598 BsaBI GATnn'nnATC Cuts at: 5692 5692 Size: 5698 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3505 3887 4522 275 Size: 53 83 186 936 1972 382 635 1451 Fragments arranged by size: 1972 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4131 4529 4803 4950 5361 4131 Size: 398 274 147 411 4468 Fragments arranged by size: 4468 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5473 Fragments arranged by size: 5473 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2615 1186 Size: 1429 4269 BseYI C'CCAG_C Cuts at: 2454 4901 5397 2454 Size: 2447 496 2755 Fragments arranged by size: 2755 2447 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5125 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3148 3909 4937 1540 Size: 275 186 1147 761 1028 2301 Fragments arranged by size: 2301 1147 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3759 3844 4915 3759 Size: 85 1071 4542 Fragments arranged by size: 4542 1071 85 BsmI GAATG_Cn' Cuts at: 3209 3302 3209 Size: 93 5605 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 3017 3759 3844 4915 1391 Size: 399 271 956 742 85 1071 2174 Fragments arranged by size: 2174 1071 956 742 399 271 85 BspEI T'CCGG_A Cuts at: 5361 5361 Size: 5698 BspHI T'CATG_A Cuts at: 2415 3587 5317 2415 Size: 1172 1730 2796 Fragments arranged by size: 2796 1730 1172 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3053 844 Size: 2209 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3585 1394 Size: 429 1762 3507 Fragments arranged by size: 3507 1762 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2140 4193 4367 65 Size: 1183 892 2053 174 1396 Fragments arranged by size: 2053 1396 1183 892 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4342 4529 1700 Size: 110 2532 187 2869 Fragments arranged by size: 2869 2532 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5698 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5698 BssSI C'ACGA_G Cuts at: 3758 4770 3758 Size: 1012 4686 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5698 BstBI TT'CG_AA Cuts at: 4502 4502 Size: 5698 BstEII G'GTnAC_C Cuts at: 1184 2201 2755 1184 Size: 1017 554 4127 Fragments arranged by size: 4127 1017 554 BstXI CCAn_nnnn'nTGG Cuts at: 2596 2596 Size: 5698 BstYI r'GATC_y Cuts at: 1972 3028 3395 3780 3797 5249 5335 5347 5693 Size: 1056 367 385 17 1452 86 12 346 Cuts at: 5693 1972 Size: 1977 Fragments arranged by size: 1977 1452 1056 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5146 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2784 487 Size: 683 56 1558 3401 Fragments arranged by size: 3401 1558 683 56 BtsI GCAGTG_nn' Cuts at: 2561 2652 2838 3214 4008 4028 2561 Size: 91 186 376 794 20 4231 Fragments arranged by size: 4231 794 376 186 91 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5698 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5663 DraI TTT'AAA Cuts at: 3002 3002 Size: 5698 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4705 809 Size: 507 3389 1802 Fragments arranged by size: 3389 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2382 2795 3145 Size: 54 1425 50 275 18 552 413 350 Cuts at: 3145 4034 5663 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 552 413 350 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3145 1537 Size: 275 1333 4090 Fragments arranged by size: 4090 1333 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2768 3628 1353 Size: 1415 860 3423 Fragments arranged by size: 3423 1415 860 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2558 4285 4659 4805 387 Size: 1064 138 969 1727 374 146 1280 Fragments arranged by size: 1727 1280 1064 969 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2618 2691 3761 4358 5145 5413 1239 Size: 393 986 73 1070 597 787 268 1524 Fragments arranged by size: 1524 1070 986 787 597 393 268 73 EcoO109I rG'GnC_Cy Cuts at: 2334 2334 Size: 5698 EcoRI G'AATT_C Cuts at: 3007 3385 3007 Size: 378 5320 FspI TGC'GCA Cuts at: 4204 4204 Size: 5698 HaeII r_GCGC'y Cuts at: 1678 4845 1678 Size: 3167 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4339 4371 4413 4445 1373 Size: 32 135 32 2767 32 42 32 2626 Fragments arranged by size: 2767 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3042 3288 669 Size: 988 281 1104 246 3079 Fragments arranged by size: 3079 1104 988 281 246 HindIII A'AGCT_T Cuts at: 748 2910 748 Size: 2162 3536 HpaI GTT'AAC Cuts at: 3288 3288 Size: 5698 KpnI G_GTAC'C Cuts at: 3023 3023 Size: 5698 MfeI C'AATT_G Cuts at: 3297 3297 Size: 5698 MluI A'CGCG_T Cuts at: 5574 5574 Size: 5698 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2060 4812 4996 1305 Size: 755 2752 184 2007 Fragments arranged by size: 2752 2007 755 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2384 10 Size: 54 1425 343 552 3324 Fragments arranged by size: 3324 1425 552 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2028 2657 3656 4015 4174 518 Size: 648 862 629 999 359 159 2042 Fragments arranged by size: 2042 999 862 648 629 359 159 MspA1I CmG'CkG Cuts at: 1606 3114 3791 4939 5184 1606 Size: 1508 677 1148 245 2120 Fragments arranged by size: 2120 1508 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5146 NdeI CA'TA_TG Cuts at: 387 387 Size: 5698 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5698 NotI GC'GGCC_GC Cuts at: 3145 3145 Size: 5698 NspI r_CATG'y Cuts at: 1132 1229 2301 2926 3056 4601 1132 Size: 97 1072 625 130 1545 2229 Fragments arranged by size: 2229 1545 1072 625 130 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5698 PciI A'CATG_T Cuts at: 2922 4597 2922 Size: 1675 4023 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5698 PmeI GTTT'AAAC Cuts at: 3002 3002 Size: 5698 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3767 3799 5280 5312 3767 Size: 32 1481 32 4153 Fragments arranged by size: 4153 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5698 PsiI TTA'TAA Cuts at: 3268 3268 Size: 5698 PstI C_TGCA'G Cuts at: 830 3050 830 Size: 2220 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5698 SalI G'TCGA_C Cuts at: 1936 3040 1936 Size: 1104 4594 SbfI CC_TGCA'GG Cuts at: 3050 3050 Size: 5698 ScaI AGT'ACT Cuts at: 3946 5658 3946 Size: 1712 3986 SfcI C'TryA_G Cuts at: 826 1045 2174 3046 4181 4862 5053 826 Size: 219 1129 872 1135 681 191 1471 Fragments arranged by size: 1471 1135 1129 872 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5698 SmaI CCC'GGG Cuts at: 1872 3025 1872 Size: 1153 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2269 3802 4703 4965 820 Size: 197 926 326 1533 901 262 1553 Fragments arranged by size: 1553 1533 926 901 326 262 197 SnaBI TAC'GTA Cuts at: 493 2891 3489 493 Size: 2398 598 2702 Fragments arranged by size: 2702 2398 598 SpeI A'CTAG_T Cuts at: 152 152 Size: 5698 SphI G_CATG'C Cuts at: 1132 3056 1132 Size: 1924 3774 SspI AAT'ATT Cuts at: 5 52 3622 5 Size: 47 3570 2081 Fragments arranged by size: 3570 2081 47 StuI AGG'CCT Cuts at: 1517 2723 1517 Size: 1206 4492 StyI C'CwwG_G Cuts at: 513 1065 2445 3137 513 Size: 552 1380 692 3074 Fragments arranged by size: 3074 1380 692 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2321 2446 3725 3742 3895 4080 4525 Size: 1255 741 125 1279 17 153 185 445 Cuts at: 4525 325 Size: 1498 Fragments arranged by size: 1498 1279 1255 741 445 185 153 125 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2187 3944 5656 Size: 274 80 33 51 1443 210 1757 1712 Cuts at: 5656 96 Size: 138 Fragments arranged by size: 1757 1712 1443 274 210 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2270 2302 5658 5690 2270 Size: 32 3356 32 2278 Fragments arranged by size: 3356 2278 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2612 1316 Size: 1296 4402 XbaI T'CTAG_A Cuts at: 3034 3034 Size: 5698 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2494 2494 Size: 5698 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5698 XmaI C'CCGG_G Cuts at: 1870 3023 1870 Size: 1153 4545 XmnI GAAnn'nnTTC Cuts at: 2723 2873 3827 2723 Size: 150 954 4594 Fragments arranged by size: 4594 954 150 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3506 276 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI