################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspLU11I BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CfoI ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HinfI HpaI KasI KpnI Ksp632I KspI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MunI MvnI NarI NciI NcoI NdeII NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TfiI Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AfaI 472 GTAC GT^AC AlwNI 152 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 31 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 679 GAGCAC G[AT]GC[AT]^C AspI 51 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 754 GGACC G^G[AT]CC BanII 160 GGGCTC G[GA]GC[CT]^C BbvI 287 GCTGC GCAGC(8/12) BlpI 615 GCTCAGC GC^T[ACGT]AGC Bpu1102I 615 GCTCAGC GC^T[ACGT]AGC BsaI 597 GGTCTC GGTCTC(1/5) BsiHKAI 679 GAGCAC G[AT]GC[AT]^C Bsp1407I 471 TGTACA T^GTACA BspHI 404 TCATGA T^CATGA BspMI 756 ACCTGC ACCTGC(4/8) BsrBI 323 GAGCGG CCGCTC(-3/-3) BsrFI 380 GCCGGC [GA]^CCGG[CT] BsrGI 471 TGTACA T^GTACA BstXI 236 CCAGGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 615 GCTCAGC GC^T[ACGT]AGC Cfr10I 380 GCCGGC [GA]^CCGG[CT] EcoRI 31 GAATTC G^AATTC FauI 325 GCGGG CCCGC(4/6) Fnu4HI 287 GCTGC GC^[ACGT]GC HapII 381 CCGG C^CGG HincII 908 GTCAAC GT[CT]^[GA]AC HindII 908 GTCAAC GT[CT]^[GA]AC HindIII 884 AAGCTT A^AGCTT HpaII 381 CCGG C^CGG ItaI 287 GCTGC GC^[ACGT]GC MaeIII 25 GTGAC ^GT[ACGT]AC MslI 18 CACAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 797 CAGCTG C[AC]G^C[GT]G MspI 381 CCGG C^CGG NaeI 380 GCCGGC GCC^GGC NdeI 913 CATATG CA^TATG NgoMIV 380 GCCGGC G^CCGGC NlaIV 231 GGTTCC GG[ACGT]^[ACGT]CC NspI 715 ACATGC [GA]CATG^[CT] PflFI 51 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 797 CAGCTG CAG^CTG RcaI 404 TCATGA T^CATGA RsaI 472 GTAC GT^AC SfcI 279 CTATAG C^T[GA][CT]AG SmlI 59 CTCAAG C^T[CT][GA]AG SspBI 471 TGTACA T^GTACA TaqI 521 TCGA T^CGA TseI 287 GCTGC G^C[AT]GC Tsp45I 25 GTGAC ^GT[GC]AC Tth111I 51 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 521 TCGA T^CGA XmnI 31 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) 325 GCGG AcsI 31 GAATTC [GA]^AATT[CT] 123 AAATTT ApoI 31 GAATTC [GA]^AATT[CT] 123 AAATTT BfaI 88 CTAG C^TAG 413 CTAG BmyI 160 GGGCTC G[AGT]GC[ACT]^C 679 GAGCAC BseRI 340 CTCCTC GAGGAG(10/8) 684 CTCCTC BsgI 490 CTGCAC GTGCAG(16/14) 564 CTGCAC BsmAI 57 GTCTC GTCTC(1/5) 598 GTCTC Bsp1286I 160 GGGCTC G[AGT]GC[ACT]^C 679 GAGCAC BsrI 204 ACTGG ACTGG(1/-1) 54 CCAGT EcoT22I 209 ATGCAT ATGCA^T 425 ATGCAT MaeI 88 CTAG C^TAG 413 CTAG MseI 94 TTAA T^TAA 904 TTAA NsiI 209 ATGCAT ATGCA^T 425 ATGCAT Tru9I 94 TTAA T^TAA 904 TTAA TspRI 20 CAGTG CA[GC]TG[ACGT][ACGT]^ 606 CAGTG XcmI 236 CCAGGATGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 569 CCAATACCTTTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AluI BsaJI BsiYI BslI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRII FokI HaeIII HphI MboII MnlI MvaI MwoI NlaIII Sau96I ScrFI SfaNI Tsp509I