(Circular) (Six-base) MAPSORT of: pFN21AE5389.seq Check: 4664 from: 1 to: 5629 pFN21AE5389 5629 bp With 182 enzymes: SgfI * January 30, 2014 17:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3439 278 Size: 53 83 186 936 1903 2468 Fragments arranged by size: 2468 1903 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2972 1937 Size: 1035 4594 Acc65I G'GTAC_C Cuts at: 2950 2950 Size: 5629 AclI AA'CG_TT Cuts at: 1563 3756 4129 1563 Size: 2193 373 3063 Fragments arranged by size: 3063 2193 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2715 3692 5076 5344 1632 Size: 1083 977 1384 268 1917 Fragments arranged by size: 1917 1384 1083 977 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5432 AflIII A'CryG_T Cuts at: 1203 4528 5505 1203 Size: 3325 977 1327 Fragments arranged by size: 3325 1327 977 AgeI A'CCGG_T Cuts at: 4460 4460 Size: 5629 AhdI GACnn_n'nnGTC Cuts at: 4358 4358 Size: 5629 AleI CACnn'nnGTG Cuts at: 1166 2027 1166 Size: 861 4768 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5629 AlwNI CAG_nnn'CTG Cuts at: 2162 3372 4944 2162 Size: 1210 1572 2847 Fragments arranged by size: 2847 1572 1210 ApaLI G'TGCA_C Cuts at: 3686 4842 3686 Size: 1156 4473 ApoI r'AATT_y Cuts at: 1493 2036 2128 2938 3164 3316 5425 1493 Size: 543 92 810 226 152 2109 1697 Fragments arranged by size: 2109 1697 810 543 226 152 92 AseI AT'TA_AT Cuts at: 160 1032 4183 160 Size: 872 3151 1606 Fragments arranged by size: 3151 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 AvaI C'yCGr_G Cuts at: 1870 1943 2954 1870 Size: 73 1011 4545 Fragments arranged by size: 4545 1011 73 BamHI G'GATC_C Cuts at: 2959 3326 2959 Size: 367 5262 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2950 4405 618 Size: 325 217 627 1163 1455 1842 Fragments arranged by size: 1842 1455 1163 627 325 217 BanII G_rGCy'C Cuts at: 1391 2169 2948 1391 Size: 778 779 4072 Fragments arranged by size: 4072 779 778 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4658 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3820 3854 1903 Size: 34 1883 34 3678 Fragments arranged by size: 3678 1883 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3521 4731 3521 Size: 1210 4419 BclI T'GATC_A Cuts at: 1548 1548 Size: 5629 BfrBI ATG'CAT Cuts at: 2216 2432 2216 Size: 216 5413 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4240 136 Size: 107 122 71 631 605 2568 1525 Fragments arranged by size: 2568 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5624 5624 Size: 5629 BlpI GC'TnA_GC Cuts at: 2621 3046 2621 Size: 425 5204 Bme1580I G_kGCm'C Cuts at: 1790 3690 4846 1790 Size: 1900 1156 2573 Fragments arranged by size: 2573 1900 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5629 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2053 3360 4318 Size: 416 53 83 399 24 636 1307 958 Cuts at: 4318 442 Size: 1753 Fragments arranged by size: 1753 1307 958 636 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5629 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2707 2707 Size: 5629 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4289 1239 Size: 3050 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2049 3754 4619 4917 2049 Size: 1705 865 298 2761 Fragments arranged by size: 2761 1705 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2608 4292 882 Size: 617 1109 1684 2219 Fragments arranged by size: 2219 1684 1109 617 BsaAI yAC'GTr Cuts at: 493 1204 3420 493 Size: 711 2216 2702 Fragments arranged by size: 2702 2216 711 BsaBI GATnn'nnATC Cuts at: 5623 5623 Size: 5629 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3436 3818 4453 275 Size: 53 83 186 936 1903 382 635 1451 Fragments arranged by size: 1903 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4062 4460 4734 4881 5292 4062 Size: 398 274 147 411 4399 Fragments arranged by size: 4399 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5404 Fragments arranged by size: 5404 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2336 2680 1186 Size: 1150 344 4135 Fragments arranged by size: 4135 1150 344 BseYI C'CCAG_C Cuts at: 2800 4832 5328 2800 Size: 2032 496 3101 Fragments arranged by size: 3101 2032 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2480 2554 1163 Size: 573 744 74 4238 Fragments arranged by size: 4238 744 573 74 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3079 3840 4868 1540 Size: 275 186 1078 761 1028 2301 Fragments arranged by size: 2301 1078 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2688 3690 3775 4846 2688 Size: 1002 85 1071 3471 Fragments arranged by size: 3471 1071 1002 85 BsmI GAATG_Cn' Cuts at: 3140 3233 3140 Size: 93 5536 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2688 2948 3690 3775 4846 1391 Size: 399 379 519 260 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 519 399 379 260 85 BspEI T'CCGG_A Cuts at: 5292 5292 Size: 5629 BspHI T'CATG_A Cuts at: 2409 3518 5248 2409 Size: 1109 1730 2790 Fragments arranged by size: 2790 1730 1109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2770 2984 844 Size: 1926 214 3489 Fragments arranged by size: 3489 1926 214 BsrBI CCG'CTC Cuts at: 1394 1823 2330 3516 1394 Size: 429 507 1186 3507 Fragments arranged by size: 3507 1186 507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4124 4298 65 Size: 1183 2876 174 1396 Fragments arranged by size: 2876 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2385 4273 4460 1700 Size: 110 575 1888 187 2869 Fragments arranged by size: 2869 1888 575 187 110 BsrGI T'GTAC_A Cuts at: 96 2476 96 Size: 2380 3249 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5629 BssSI C'ACGA_G Cuts at: 3689 4701 3689 Size: 1012 4617 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5629 BstBI TT'CG_AA Cuts at: 4433 4433 Size: 5629 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5629 BstXI CCAn_nnnn'nTGG Cuts at: 2248 2248 Size: 5629 BstYI r'GATC_y Cuts at: 1972 2959 3326 3711 3728 5180 5266 5278 5624 Size: 987 367 385 17 1452 86 12 346 Cuts at: 5624 1972 Size: 1977 Fragments arranged by size: 1977 1452 987 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4138 Fragments arranged by size: 4138 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2359 487 Size: 683 56 1133 3757 Fragments arranged by size: 3757 1133 683 56 BtsI GCAGTG_nn' Cuts at: 3145 3939 3959 3145 Size: 794 20 4815 Fragments arranged by size: 4815 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5629 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5594 DraI TTT'AAA Cuts at: 2933 2933 Size: 5629 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4636 809 Size: 507 3320 1802 Fragments arranged by size: 3320 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3076 3965 5594 Size: 54 1425 50 275 18 1246 889 1629 Cuts at: 5594 8 Size: 43 Fragments arranged by size: 1629 1425 1246 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3076 1537 Size: 275 1264 4090 Fragments arranged by size: 4090 1264 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3559 1353 Size: 2206 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4216 4590 4736 387 Size: 1064 138 2627 374 146 1280 Fragments arranged by size: 2627 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2715 3692 4289 5076 5344 1239 Size: 393 1083 977 597 787 268 1524 Fragments arranged by size: 1524 1083 977 787 597 393 268 EcoRI G'AATT_C Cuts at: 2036 2938 3316 2036 Size: 902 378 4349 Fragments arranged by size: 4349 902 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2504 2504 Size: 5629 FspI TGC'GCA Cuts at: 4135 4135 Size: 5629 HaeII r_GCGC'y Cuts at: 1678 4776 1678 Size: 3098 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2047 2079 2246 2278 4270 Size: 32 135 32 475 32 167 32 1992 Cuts at: 4270 4302 4344 4376 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1992 475 167 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2915 2973 3219 669 Size: 988 281 977 58 246 3079 Fragments arranged by size: 3079 988 977 281 246 58 HindIII A'AGCT_T Cuts at: 748 2889 748 Size: 2141 3488 HpaI GTT'AAC Cuts at: 3219 3219 Size: 5629 KpnI G_GTAC'C Cuts at: 2954 2954 Size: 5629 MfeI C'AATT_G Cuts at: 3228 3228 Size: 5629 MluI A'CGCG_T Cuts at: 5505 5505 Size: 5629 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2631 4743 4927 1305 Size: 1326 2112 184 2007 Fragments arranged by size: 2112 2007 1326 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3807 Fragments arranged by size: 3807 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 3587 3946 4105 518 Size: 648 861 1560 359 159 2042 Fragments arranged by size: 2042 1560 861 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2804 3045 3722 4870 5115 1606 Size: 1198 241 677 1148 245 2120 Fragments arranged by size: 2120 1198 1148 677 245 241 NaeI GCC'GGC Cuts at: 2387 2387 Size: 5629 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4138 Fragments arranged by size: 4138 939 552 NdeI CA'TA_TG Cuts at: 387 2919 387 Size: 2532 3097 NgoMIV G'CCGG_C Cuts at: 2385 2385 Size: 5629 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5629 NotI GC'GGCC_GC Cuts at: 3076 3076 Size: 5629 NsiI A_TGCA'T Cuts at: 2218 2434 2218 Size: 216 5413 NspI r_CATG'y Cuts at: 1132 1229 2724 2987 4532 1132 Size: 97 1495 263 1545 2229 Fragments arranged by size: 2229 1545 1495 263 97 PasI CC'CwG_GG Cuts at: 1348 2162 1348 Size: 814 4815 PciI A'CATG_T Cuts at: 4528 4528 Size: 5629 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5629 PmeI GTTT'AAAC Cuts at: 2933 2933 Size: 5629 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3698 3730 5211 5243 3698 Size: 32 1481 32 4084 Fragments arranged by size: 4084 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5629 PsiI TTA'TAA Cuts at: 3199 3199 Size: 5629 PstI C_TGCA'G Cuts at: 830 2981 830 Size: 2151 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5629 PvuII CAG'CTG Cuts at: 2804 2804 Size: 5629 SalI G'TCGA_C Cuts at: 1936 2971 1936 Size: 1035 4594 SbfI CC_TGCA'GG Cuts at: 2981 2981 Size: 5629 ScaI AGT'ACT Cuts at: 3877 5589 3877 Size: 1712 3917 SfcI C'TryA_G Cuts at: 826 1045 2284 2977 4112 4793 4984 826 Size: 219 1239 693 1135 681 191 1471 Fragments arranged by size: 1471 1239 1135 693 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5629 SmaI CCC'GGG Cuts at: 1872 2956 1872 Size: 1084 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2064 3733 4634 4896 820 Size: 197 926 121 1669 901 262 1553 Fragments arranged by size: 1669 1553 926 901 262 197 121 SnaBI TAC'GTA Cuts at: 493 3420 493 Size: 2927 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5629 SphI G_CATG'C Cuts at: 1132 2987 1132 Size: 1855 3774 SspI AAT'ATT Cuts at: 5 52 3553 5 Size: 47 3501 2081 Fragments arranged by size: 3501 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5629 StyI C'CwwG_G Cuts at: 513 1065 2004 3068 513 Size: 552 939 1064 3074 Fragments arranged by size: 3074 1064 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2744 3656 3673 3826 4011 4456 325 Size: 1255 1164 912 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1164 912 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2476 3875 5587 Size: 274 80 33 51 1443 499 1399 1712 Cuts at: 5587 96 Size: 138 Fragments arranged by size: 1712 1443 1399 499 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5589 5621 5589 Size: 32 5597 Tth111I GACn'n_nGTC Cuts at: 1316 2059 1316 Size: 743 4886 XbaI T'CTAG_A Cuts at: 2965 2965 Size: 5629 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2248 2581 2248 Size: 333 5296 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5629 XmaI C'CCGG_G Cuts at: 1870 2954 1870 Size: 1084 4545 XmnI GAAnn'nnTTC Cuts at: 2040 3758 2040 Size: 1718 3911 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3437 276 Size: 53 83 186 936 1903 2468 Fragments arranged by size: 2468 1903 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI