Restriction map for
pFN21AE5489
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstYI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I FauI FbaI
Fnu4HI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI HpaII ItaI KasI
KpnI KspI MaeII MamI MfeI
MflI MluI MroI MspA1I MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI TseI
Tth111I TthHB8I Van91I XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 613 GTAGAC GT^[AC][GT]AC
AlwI 753 GATCC GGATC(4/5)
AocI 328 CCTAAGG CC^T[ACGT]AGG
AvaII 601 GGTCC G^G[AT]CC
BalI 464 TGGCCA TGG^CCA
BfaI 1016 CTAG C^TAG
BpmI 996 CTGGAG CTGGAG(16/14)
Bsp1407I 675 TGTACA T^GTACA
BspHI 497 TCATGA T^CATGA
BsrGI 675 TGTACA T^GTACA
BssKI 153 CCAGG ^CC[ACGT]GG
BstEII 214 GGTAACC G^GT[ACGT]ACC
BstNI 153 CCAGG CC^[AT]GG
BstPI 214 GGTAACC G^GT[ACGT]ACC
Bsu36I 328 CCTAAGG CC^T[ACGT]AGG
EaeI 464 TGGCCA [CT]^GGCC[GA]
Eco81I 328 CCTAAGG CC^T[ACGT]AGG
EcoO65I 214 GGTAACC G^GT[ACGT]ACC
EcoRII 153 CCAGG ^CC[AT]GG
EcoRV 978 GATATC GAT^ATC
EcoT22I 852 ATGCAT ATGCA^T
HindIII 649 AAGCTT A^AGCTT
MaeI 1016 CTAG C^TAG
MluNI 464 TGGCCA TGG^CCA
MscI 464 TGGCCA TGG^CCA
MvaI 153 CCAGG CC^[AT]GG
MwoI 313 GCTTCTTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 601 GGTCCC GG[ACGT]^[ACGT]CC
NsiI 852 ATGCAT ATGCA^T
RcaI 497 TCATGA T^CATGA
ScrFI 153 CCAGG CC^[ACGT]GG
SfaNI 854 GCATC GCATC(5/9)
SmlI 113 CTCAAG C^T[CT][GA]AG
SspBI 675 TGTACA T^GTACA
XbaI 1015 TCTAGA T^CTAGA
XcmI 774 CCACTCTCCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcsI 124 GAATTC [GA]^AATT[CT]
1011 GAATTC
AfaI 430 GTAC GT^AC
676 GTAC
ApoI 124 GAATTC [GA]^AATT[CT]
1011 GAATTC
BsaI 438 GGTCTC GGTCTC(1/5)
547 GGTCTC
BsmAI 439 GTCTC GTCTC(1/5)
548 GTCTC
BsmFI 138 GGGAC GGGAC(10/14)
602 GTCCC
BsrI 411 ACTGG ACTGG(1/-1)
784 ACTGG
Cac8I 73 GCGAGC GC[ACGT]^[ACGT]GC
651 GCTTGC
DpnII 40 GATC ^GATC
753 GATC
DsaI 27 CCATGG C^C[GA][CT]GG
443 CCATGG
EarI 181 CTCTTC CTCTTC(1/4)
838 CTCTTC
EcoRI 124 GAATTC G^AATTC
1011 GAATTC
HphI 333 GGTGA GGTGA(8/7)
831 GGTGA
Ksp632I 181 CTCTTC CTCTTC(1/4)
838 CTCTTC
MboI 40 GATC ^GATC
753 GATC
MslI 822 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
825 CACAGTGGTG
NcoI 27 CCATGG C^CATGG
443 CCATGG
NdeII 40 GATC ^GATC
753 GATC
RsaI 430 GTAC GT^AC
676 GTAC
Sau3AI 40 GATC ^GATC
753 GATC
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI BsaJI BsiYI BslI BstF5I
Cfr13I DdeI EcoT14I FokI HaeIII
HinfI MaeIII MboII MnlI MseI
NlaIII Sau96I SfcI StyI TfiI
Tru9I Tsp45I Tsp509I TspRI