################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BpmI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 677 GGTACC G^GTACC AfaI 678 GTAC GT^AC Alw44I 460 GTGCAC G^TGCAC AlwNI 586 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 460 GTGCAC G^TGCAC Asp718I 677 GGTACC G^GTACC AvaII 508 GGTCC G^G[AT]CC BanI 677 GGTACC G^G[CT][GA]CC BbsI 100 GTCTTC GAAGAC(2/6) BlpI 186 GCTTAGC GC^T[ACGT]AGC Bpu1102I 186 GCTTAGC GC^T[ACGT]AGC BpuAI 100 GTCTTC GAAGAC(2/6) BseRI 634 CTCCTC GAGGAG(10/8) BsmAI 646 GTCTC GTCTC(1/5) BspHI 404 TCATGA T^CATGA BspMI 125 ACCTGC ACCTGC(4/8) BsrDI 597 CATTGC GCAATG(2/0) Cac8I 562 GCCTGC GC[ACGT]^[ACGT]GC CelII 186 GCTTAGC GC^T[ACGT]AGC EcoT14I 350 CCATGG C^C[AT][AT]GG HaeIII 779 GGCC GG^CC HincII 489 GTTGAC GT[CT]^[GA]AC HindII 489 GTTGAC GT[CT]^[GA]AC HphI 138 GGTGA GGTGA(8/7) KpnI 677 GGTACC GGTAC^C NcoI 350 CCATGG C^CATGG NdeI 375 CATATG CA^TATG RcaI 404 TCATGA T^CATGA RsaI 678 GTAC GT^AC SfcI 583 CTACAG C^T[GA][CT]AG SspI 663 AATATT AAT^ATT StyI 350 CCATGG C^C[AT][AT]GG TaqI 693 TCGA T^CGA TthHB8I 693 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 31 GAATTT [GA]^AATT[CT] 806 AAATTC AlwI 213 GATCC GGATC(4/5) 695 GATCC ApoI 31 GAATTT [GA]^AATT[CT] 806 AAATTC AspHI 418 GTGCTC G[AT]GC[AT]^C 460 GTGCAC BanII 408 GAGCCC G[GA]GC[CT]^C 610 GGGCTC BbvI 886 GCAGC GCAGC(8/12) 685 GCTGC BfaI 470 CTAG C^TAG 689 CTAG BsiHKAI 418 GTGCTC G[AT]GC[AT]^C 460 GTGCAC BsmFI 45 GGGAC GGGAC(10/14) 509 GTCCC BsrI 424 ACTGG ACTGG(1/-1) 621 ACTGG BstF5I 579 CATCC GGATG(2/0) 286 GGATG Cfr13I 508 GGTCC G^G[ACGT]CC 779 GGCCC DpnII 213 GATC ^GATC 695 GATC DsaI 350 CCATGG C^C[GA][CT]GG 412 CCACGG Fnu4HI 685 GCTGC GC^[ACGT]GC 886 GCAGC FokI 579 CATCC GGATG(9/13) 286 GGATG ItaI 685 GCTGC GC^[ACGT]GC 886 GCAGC MaeI 470 CTAG C^TAG 689 CTAG MaeII 264 ACGT A^CGT 773 ACGT MboI 213 GATC ^GATC 695 GATC MwoI 220 GCTTCTTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 420 GCTCACTGGGC NdeII 213 GATC ^GATC 695 GATC PflMI 15 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 272 CCATCTCATGG Sau3AI 213 GATC ^GATC 695 GATC Sau96I 508 GGTCC G^G[ACGT]CC 779 GGCCC TfiI 89 GATTC G^A[AT]TC 154 GATTC TseI 685 GCTGC G^C[AT]GC 886 GCAGC Van91I 15 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 272 CCATCTCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AluI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI DdeI EcoRII HinfI MaeIII MboII MnlI MseI MslI MvaI NlaIII NlaIV PleI ScrFI Tru9I Tsp45I Tsp509I TspRI