################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraII DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MroI MseI MspA1I MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccI 523 GTAGAC GT^[AC][GT]AC AfaI 180 GTAC GT^AC AflIII 212 ACATGT A^C[GA][CT]GT Alw44I 463 GTGCAC G^TGCAC ApaLI 463 GTGCAC G^TGCAC AvaII 3 GGACC G^G[AT]CC BfaI 199 CTAG C^TAG BspLU11I 212 ACATGT A^CATGT BsrFI 691 GCCGGT [GA]^CCGG[CT] BsrI 322 ACTGG ACTGG(1/-1) BstEII 121 GGTAACC G^GT[ACGT]ACC BstPI 121 GGTAACC G^GT[ACGT]ACC BstXI 110 CCATTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 691 GCCGGT [GA]^CCGG[CT] DrdI 574 GACACCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 353 CCATGG C^C[GA][CT]GG EcoO65I 121 GGTAACC G^GT[ACGT]ACC FauI 432 GCGGG CCCGC(4/6) HapII 692 CCGG C^CGG HgaI 744 GACGC GACGC(5/10) HinfI 47 GACTC G^A[ACGT]TC HpaII 692 CCGG C^CGG MaeI 199 CTAG C^TAG MslI 735 CATGGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 692 CCGG C^CGG MwoI 223 GCCTCATTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 353 CCATGG C^CATGG NdeI 378 CATATG CA^TATG NspI 212 ACATGT [GA]CATG^[CT] PleI 47 GACTC GAGTC(4/5) RsaI 180 GTAC GT^AC SfaNI 425 GCATC GCATC(5/9) SmlI 624 CTTGAG C^T[CT][GA]AG XcmI 470 CCATCAGTCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AciI 432 GCGG CCGC(-3/-1) 780 GCGG AlwI 154 GATCC GGATC(4/5) 663 GATCC AspHI 340 GTGCTC G[AT]GC[AT]^C 463 GTGCAC BalI 107 TGGCCA TGG^CCA 374 TGGCCA BmyI 340 GTGCTC G[AGT]GC[ACT]^C 463 GTGCAC BsaJI 20 CCTTGG C^C[ACGT][ACGT]GG 353 CCATGG BsiHKAI 340 GTGCTC G[AT]GC[AT]^C 463 GTGCAC Bsp1286I 340 GTGCTC G[AGT]GC[ACT]^C 463 GTGCAC BssKI 207 CCTGG ^CC[ACGT]GG 345 CCTGG BstF5I 701 CATCC GGATG(2/0) 289 GGATG BstNI 207 CCTGG CC^[AT]GG 345 CCTGG EaeI 107 TGGCCA [CT]^GGCC[GA] 374 TGGCCA EcoRII 207 CCTGG ^CC[AT]GG 345 CCTGG EcoT14I 20 CCTTGG C^C[AT][AT]GG 353 CCATGG FokI 701 CATCC GGATG(9/13) 289 GGATG HphI 552 GGTGA GGTGA(8/7) 918 GGTGA MluNI 107 TGGCCA TGG^CCA 374 TGGCCA MscI 107 TGGCCA TGG^CCA 374 TGGCCA MvaI 207 CCTGG CC^[AT]GG 345 CCTGG ScrFI 207 CCTGG CC^[ACGT]GG 345 CCTGG StyI 20 CCTTGG C^C[AT][AT]GG 353 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AcsI AluI ApoI BbvI BclI BseRI BsgI BsiYI BslI Cac8I Cfr13I DdeI DpnII FbaI Fnu4HI HaeIII ItaI MaeIII MboI MboII MnlI NdeII NlaIII NlaIV Sau3AI Sau96I TseI Tsp45I Tsp509I TspRI