################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 247 GTAGAC GT^[AC][GT]AC AciI 448 GCGG CCGC(-3/-1) AcsI 805 AAATTT [GA]^AATT[CT] ApoI 805 AAATTT [GA]^AATT[CT] AspHI 262 GAGCAC G[AT]GC[AT]^C BbsI 788 GAAGAC GAAGAC(2/6) BbvI 886 GCAGC GCAGC(8/12) BciVI 570 GGATAC GTATCC(6/5) BfaI 118 CTAG C^TAG BmyI 262 GAGCAC G[AGT]GC[ACT]^C BpmI 687 CTCCAG CTGGAG(16/14) BpuAI 788 GAAGAC GAAGAC(2/6) BsaWI 410 ACCGGA [AT]^CCGG[AT] BsgI 388 CTGCAC GTGCAG(16/14) BsiHKAI 262 GAGCAC G[AT]GC[AT]^C Bsp1286I 262 GAGCAC G[AGT]GC[ACT]^C BspDI 18 ATCGAT AT^CGAT BsrDI 122 GCAATG GCAATG(2/0) BsrI 689 CCAGT ACTGG(1/-1) BstXI 512 CCAATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 18 ATCGAT AT^CGAT DpnII 666 GATC ^GATC DraII 682 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoO109I 682 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoT14I 545 CCTTGG C^C[AT][AT]GG Fnu4HI 886 GCAGC GC^[ACGT]GC HapII 411 CCGG C^CGG HpaII 411 CCGG C^CGG HphI 549 GGTGA GGTGA(8/7) ItaI 886 GCAGC GC^[ACGT]GC MaeI 118 CTAG C^TAG MboI 666 GATC ^GATC MspI 411 CCGG C^CGG NdeII 666 GATC ^GATC NlaIV 508 GGTCCC GG[ACGT]^[ACGT]CC PflMI 499 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 682 AGGTCCT [GA]G^G[AT]CC[CT] Sau3AI 666 GATC ^GATC StyI 545 CCTTGG C^C[AT][AT]GG TaqI 19 TCGA T^CGA TfiI 16 GAATC G^A[AT]TC TseI 886 GCAGC G^C[AT]GC TthHB8I 19 TCGA T^CGA Van91I 499 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### BspMI 494 ACCTGC ACCTGC(4/8) 680 GCAGGT Cac8I 558 GCTGGC GC[ACGT]^[ACGT]GC 562 GCTTGC EcoRV 208 GATATC GAT^ATC 615 GATATC MaeIII 106 GTGAC ^GT[ACGT]AC 536 GTGAC MslI 7 CACACAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 729 CATCACAGTG PleI 47 GACTC GAGTC(4/5) 154 GACTC SfaNI 240 GATGC GCATC(5/9) 677 GATGC SfcI 95 CTATAG C^T[GA][CT]AG 393 CTACAG Tsp45I 106 GTGAC ^GT[GC]AC 536 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AfaI AluI AvaII BsaJI BsiYI BslI BsmFI BssKI BstNI Cfr13I DdeI EcoRII HaeIII HinfI MboII MnlI MseI MvaI NdeI NlaIII RsaI Sau96I ScrFI Tru9I Tsp509I TspRI