(Circular) (Six-base) MAPSORT of: pFN21AE5922.seq Check: 4397 from: 1 to: 5638 pFN21AE5922 5638 bp With 182 enzymes: SgfI * December 8, 2014 13:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3448 278 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2253 2981 1937 Size: 316 728 4594 Fragments arranged by size: 4594 728 316 Acc65I G'GTAC_C Cuts at: 2959 2959 Size: 5638 AclI AA'CG_TT Cuts at: 1563 2937 3765 4138 1563 Size: 1374 828 373 3063 Fragments arranged by size: 3063 1374 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3701 5085 5353 1632 Size: 2069 1384 268 1917 Fragments arranged by size: 2069 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5441 AflIII A'CryG_T Cuts at: 1203 4537 5514 1203 Size: 3334 977 1327 Fragments arranged by size: 3334 1327 977 AgeI A'CCGG_T Cuts at: 4469 4469 Size: 5638 AhdI GACnn_n'nnGTC Cuts at: 4367 4367 Size: 5638 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5638 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5638 AlwNI CAG_nnn'CTG Cuts at: 3381 4953 3381 Size: 1572 4066 ApaLI G'TGCA_C Cuts at: 3695 4851 3695 Size: 1156 4482 ApoI r'AATT_y Cuts at: 1493 2810 2947 3173 3325 5434 1493 Size: 1317 137 226 152 2109 1697 Fragments arranged by size: 2109 1697 1317 226 152 137 AseI AT'TA_AT Cuts at: 160 1032 4192 160 Size: 872 3160 1606 Fragments arranged by size: 3160 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 AvaI C'yCGr_G Cuts at: 1870 1943 2963 1870 Size: 73 1020 4545 Fragments arranged by size: 4545 1020 73 BamHI G'GATC_C Cuts at: 2968 3335 2968 Size: 367 5271 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2959 4414 618 Size: 325 217 627 1172 1455 1842 Fragments arranged by size: 1842 1455 1172 627 325 217 BanII G_rGCy'C Cuts at: 1391 2957 1391 Size: 1566 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2800 928 Size: 971 901 3766 Fragments arranged by size: 3766 971 901 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3829 3863 1903 Size: 34 1892 34 3678 Fragments arranged by size: 3678 1892 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2569 3530 4740 2569 Size: 961 1210 3467 Fragments arranged by size: 3467 1210 961 BclI T'GATC_A Cuts at: 1548 1548 Size: 5638 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4249 136 Size: 107 122 71 631 605 2577 1525 Fragments arranged by size: 2577 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5633 5633 Size: 5638 BlpI GC'TnA_GC Cuts at: 3055 3055 Size: 5638 Bme1580I G_kGCm'C Cuts at: 1790 3699 4855 1790 Size: 1909 1156 2573 Fragments arranged by size: 2573 1909 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5638 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3369 4327 442 Size: 416 53 83 399 24 1952 958 1753 Fragments arranged by size: 1952 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5638 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2677 4298 1239 Size: 1438 1621 2579 Fragments arranged by size: 2579 1621 1438 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3763 4628 4926 3763 Size: 865 298 4475 Fragments arranged by size: 4475 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4301 882 Size: 617 2802 2219 Fragments arranged by size: 2802 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3429 493 Size: 711 2225 2702 Fragments arranged by size: 2702 2225 711 BsaBI GATnn'nnATC Cuts at: 5632 5632 Size: 5638 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3445 3827 4462 275 Size: 53 83 186 936 1912 382 635 1451 Fragments arranged by size: 1912 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2415 4071 4469 4743 4890 5301 2415 Size: 1656 398 274 147 411 2752 Fragments arranged by size: 2752 1656 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2410 2440 1178 Size: 30 165 30 1007 30 4376 Fragments arranged by size: 4376 1007 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5638 BseYI C'CCAG_C Cuts at: 4841 5337 4841 Size: 496 5142 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2378 1163 Size: 573 642 4423 Fragments arranged by size: 4423 642 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3088 3849 4877 1540 Size: 275 186 1087 761 1028 2301 Fragments arranged by size: 2301 1087 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2271 3699 3784 4855 2271 Size: 1428 85 1071 3054 Fragments arranged by size: 3054 1428 1071 85 BsmI GAATG_Cn' Cuts at: 3149 3242 3149 Size: 93 5545 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2271 2957 3699 3784 4855 1391 Size: 399 481 686 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 686 481 399 85 BspEI T'CCGG_A Cuts at: 5301 5301 Size: 5638 BspHI T'CATG_A Cuts at: 3527 5257 3527 Size: 1730 3908 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2508 2676 2993 844 Size: 1664 168 317 3489 Fragments arranged by size: 3489 1664 317 168 BsrBI CCG'CTC Cuts at: 1394 1823 3525 1394 Size: 429 1702 3507 Fragments arranged by size: 3507 1702 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2134 4133 4307 65 Size: 1183 886 1999 174 1396 Fragments arranged by size: 1999 1396 1183 886 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4282 4469 1700 Size: 110 2472 187 2869 Fragments arranged by size: 2869 2472 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5638 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5638 BssSI C'ACGA_G Cuts at: 3698 4710 3698 Size: 1012 4626 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5638 BstBI TT'CG_AA Cuts at: 4442 4442 Size: 5638 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5638 BstXI CCAn_nnnn'nTGG Cuts at: 2524 2524 Size: 5638 BstYI r'GATC_y Cuts at: 1972 2968 3335 3720 3737 5189 5275 5287 5633 Size: 996 367 385 17 1452 86 12 346 Cuts at: 5633 1972 Size: 1977 Fragments arranged by size: 1977 1452 996 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4147 Fragments arranged by size: 4147 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4899 Fragments arranged by size: 4899 683 56 BtsI GCAGTG_nn' Cuts at: 3154 3948 3968 3154 Size: 794 20 4824 Fragments arranged by size: 4824 794 20 ClaI AT'CG_AT Cuts at: 1554 2024 1554 Size: 470 5168 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5603 DraI TTT'AAA Cuts at: 2942 2942 Size: 5638 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4645 809 Size: 507 3329 1802 Fragments arranged by size: 3329 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3085 3974 5603 Size: 54 1425 50 275 18 1255 889 1629 Cuts at: 5603 8 Size: 43 Fragments arranged by size: 1629 1425 1255 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3085 1537 Size: 275 1273 4090 Fragments arranged by size: 4090 1273 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3568 1353 Size: 2215 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4225 4599 4745 387 Size: 1064 138 2636 374 146 1280 Fragments arranged by size: 2636 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2677 3701 4298 5085 5353 1239 Size: 393 1045 1024 597 787 268 1524 Fragments arranged by size: 1524 1045 1024 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2688 2688 Size: 5638 EcoRI G'AATT_C Cuts at: 2947 3325 2947 Size: 378 5260 EcoRV GAT'ATC Cuts at: 2215 2622 2215 Size: 407 5231 FspI TGC'GCA Cuts at: 4144 4144 Size: 5638 HaeII r_GCGC'y Cuts at: 1678 4785 1678 Size: 3107 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2899 2931 4279 4311 4353 Size: 32 135 32 1327 32 1348 32 42 Cuts at: 4353 4385 1373 Size: 32 2626 Fragments arranged by size: 2626 1348 1327 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2982 3228 669 Size: 988 281 1044 246 3079 Fragments arranged by size: 3079 1044 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5638 HpaI GTT'AAC Cuts at: 3228 3228 Size: 5638 KpnI G_GTAC'C Cuts at: 2963 2963 Size: 5638 MfeI C'AATT_G Cuts at: 3237 3237 Size: 5638 MluI A'CGCG_T Cuts at: 5514 5514 Size: 5638 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4752 4936 1305 Size: 3447 184 2007 Fragments arranged by size: 3447 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3816 Fragments arranged by size: 3816 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2016 2738 3596 3955 4114 518 Size: 648 850 722 858 359 159 2042 Fragments arranged by size: 2042 858 850 722 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3054 3731 4879 5124 1606 Size: 1448 677 1148 245 2120 Fragments arranged by size: 2120 1448 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4147 Fragments arranged by size: 4147 939 552 NdeI CA'TA_TG Cuts at: 387 2362 2381 2581 387 Size: 1975 19 200 3444 Fragments arranged by size: 3444 1975 200 19 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5638 NotI GC'GGCC_GC Cuts at: 3085 3085 Size: 5638 NspI r_CATG'y Cuts at: 1132 1229 2996 4541 1132 Size: 97 1767 1545 2229 Fragments arranged by size: 2229 1767 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5638 PciI A'CATG_T Cuts at: 4537 4537 Size: 5638 PflMI CCAn_nnn'nTGG Cuts at: 1108 2510 1108 Size: 1402 4236 PfoI T'CCnGG_A Cuts at: 2927 2927 Size: 5638 PmeI GTTT'AAAC Cuts at: 2942 2942 Size: 5638 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3707 3739 5220 5252 3707 Size: 32 1481 32 4093 Fragments arranged by size: 4093 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2688 2688 Size: 5638 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5638 PsiI TTA'TAA Cuts at: 3208 3208 Size: 5638 PstI C_TGCA'G Cuts at: 830 2990 830 Size: 2160 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5638 SalI G'TCGA_C Cuts at: 1936 2980 1936 Size: 1044 4594 SbfI CC_TGCA'GG Cuts at: 2990 2990 Size: 5638 ScaI AGT'ACT Cuts at: 3886 5598 3886 Size: 1712 3926 SfcI C'TryA_G Cuts at: 826 1045 2100 2398 2986 4121 4802 4993 826 Size: 219 1055 298 588 1135 681 191 1471 Fragments arranged by size: 1471 1135 1055 681 588 298 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5638 SmaI CCC'GGG Cuts at: 1872 2965 1872 Size: 1093 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3742 4643 4905 820 Size: 197 926 1799 901 262 1553 Fragments arranged by size: 1799 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3429 493 Size: 2936 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5638 SphI G_CATG'C Cuts at: 1132 2996 1132 Size: 1864 3774 SspI AAT'ATT Cuts at: 5 52 3562 5 Size: 47 3510 2081 Fragments arranged by size: 3510 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5638 StyI C'CwwG_G Cuts at: 513 1065 2004 2550 3077 513 Size: 552 939 546 527 3074 Fragments arranged by size: 3074 939 552 546 527 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3665 3682 3835 4020 4465 325 Size: 1255 2085 17 153 185 445 1498 Fragments arranged by size: 2085 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2041 2181 2402 Size: 274 80 33 51 1443 64 140 221 Cuts at: 2402 2837 3884 5596 96 Size: 435 1047 1712 138 Fragments arranged by size: 1712 1443 1047 435 274 221 140 138 80 64 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2410 2442 5598 5630 2410 Size: 32 3156 32 2418 Fragments arranged by size: 3156 2418 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5638 XbaI T'CTAG_A Cuts at: 2974 2974 Size: 5638 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5638 XmaI C'CCGG_G Cuts at: 1870 2963 1870 Size: 1093 4545 XmnI GAAnn'nnTTC Cuts at: 3767 3767 Size: 5638 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3446 276 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI