################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI AlwI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI SwaI TfiI Tth111I Van91I XbaI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AlwNI 282 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 915 CCTGAGG CC^T[ACGT]AGG AvaI 545 CTCGAG C^[CT]CG[GA]G BbsI 684 GTCTTC GAAGAC(2/6) BcgI 412 CGAAGGACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 946 CTAG C^TAG BpuAI 684 GTCTTC GAAGAC(2/6) BsaI 268 GAGACC GGTCTC(1/5) BsgI 163 CTGCAC GTGCAG(16/14) BsmAI 268 GAGAC GTCTC(1/5) BsmFI 45 GGGAC GGGAC(10/14) BsoBI 545 CTCGAG C^[CT]CG[GA]G BspHI 404 TCATGA T^CATGA BspMI 494 ACCTGC ACCTGC(4/8) BsrDI 694 GCAATG GCAATG(2/0) BssSI 265 CACGAG CACGAG(-5/-1) Bst1107I 102 GTATAC GTA^TAC BstZ17I 102 GTATAC GTA^TAC Bsu36I 915 CCTGAGG CC^T[ACGT]AGG Eco81I 915 CCTGAGG CC^T[ACGT]AGG EcoNI 356 CCTATGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 611 GAATTC G^AATTC EcoT14I 922 CCAAGG C^C[AT][AT]GG HincII 205 GTTGAC GT[CT]^[GA]AC HindII 205 GTTGAC GT[CT]^[GA]AC MaeI 946 CTAG C^TAG MseI 469 TTAA T^TAA MslI 768 CATCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 689 CAGCTG C[AC]G^C[GT]G NdeI 375 CATATG CA^TATG NspI 418 ACATGC [GA]CATG^[CT] PaeR7I 545 CTCGAG C^TCGAG PshAI 208 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 689 CAGCTG CAG^CTG RcaI 404 TCATGA T^CATGA SfaNI 761 GCATC GCATC(5/9) SfcI 51 CTGTAG C^T[GA][CT]AG SpeI 945 ACTAGT A^CTAGT SspI 741 AATATT AAT^ATT StyI 922 CCAAGG C^C[AT][AT]GG Tru9I 469 TTAA T^TAA XhoI 545 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 102 GTATAC GT^[AC][GT]AC 520 GTAGAC AfaI 37 GTAC GT^AC 177 GTAC Alw44I 260 GTGCAC G^TGCAC 460 GTGCAC ApaLI 260 GTGCAC G^TGCAC 460 GTGCAC Asp700I 191 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 611 GAATTCTTTC AspHI 260 GTGCAC G[AT]GC[AT]^C 460 GTGCAC AvaII 508 GGACC G^G[AT]CC 754 GGACC BmyI 260 GTGCAC G[AGT]GC[ACT]^C 460 GTGCAC BpmI 67 CTCCAG CTGGAG(16/14) 323 CTGGAG BsaJI 443 CCTGGG C^C[ACGT][ACGT]GG 922 CCAAGG BsiHKAI 260 GTGCAC G[AT]GC[AT]^C 460 GTGCAC BsiYI 356 CCTATGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 499 CCTTTTTGTGG BslI 356 CCTATGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 499 CCTTTTTGTGG Bsp1286I 260 GTGCAC G[AGT]GC[ACT]^C 460 GTGCAC BsrI 322 ACTGG ACTGG(1/-1) 474 CCAGT BssKI 443 CCTGG ^CC[ACGT]GG 567 CCTGG BstNI 443 CCTGG CC^[AT]GG 567 CCTGG DpnII 438 GATC ^GATC 538 GATC EcoRII 443 CCTGG ^CC[AT]GG 567 CCTGG MboI 438 GATC ^GATC 538 GATC MvaI 443 CCTGG CC^[AT]GG 567 CCTGG MwoI 220 GCTTCTTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 691 GCTGCAATGGC NdeII 438 GATC ^GATC 538 GATC NlaIII 405 CATG CATG^ 419 CATG RsaI 37 GTAC GT^AC 177 GTAC Sau3AI 438 GATC ^GATC 538 GATC ScrFI 443 CCTGG CC^[ACGT]GG 567 CCTGG SmlI 545 CTCGAG C^T[CT][GA]AG 679 CTCAAG TaqI 349 TCGA T^CGA 546 TCGA TthHB8I 349 TCGA T^CGA 546 TCGA XcmI 11 CCAATTCTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 788 CCATCTCTCCTTTGG XmnI 191 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 611 GAATTCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AcsI AluI ApoI BbvI Cac8I Cfr13I DdeI Fnu4HI HaeIII HinfI HphI ItaI MaeIII MboII MnlI PleI Sau96I TseI Tsp45I Tsp509I TspRI