################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsoBI BspDI BspEI BspHI BspMI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AfaI 719 GTAC GT^AC AflIII 773 ACATGT A^C[GA][CT]GT AlwI 694 GGATC GGATC(4/5) BanII 46 GGGCTC G[GA]GC[CT]^C BbsI 739 GTCTTC GAAGAC(2/6) BciVI 570 GGATAC GTATCC(6/5) BmyI 46 GGGCTC G[AGT]GC[ACT]^C BpuAI 739 GTCTTC GAAGAC(2/6) BsgI 388 CTGCAC GTGCAG(16/14) BsmAI 213 GTCTC GTCTC(1/5) BsmI 462 GCATTC GAATGC(1/-1) Bsp1286I 46 GGGCTC G[AGT]GC[ACT]^C Bsp1407I 718 TGTACA T^GTACA BspLU11I 773 ACATGT A^CATGT BsrGI 718 TGTACA T^GTACA BssSI 430 CTCGTG CACGAG(-5/-1) BstF5I 3 GGATG GGATG(2/0) Cac8I 380 GCAAGC GC[ACGT]^[ACGT]GC DraII 704 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoNI 356 CCTTTGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 704 AGGCCCT [GA]G^G[ACGT]CC[CT] FokI 3 GGATG GGATG(9/13) HphI 159 TCACC GGTGA(8/7) MaeII 493 ACGT A^CGT MwoI 220 GCTTCTTTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 650 CATATG CA^TATG NspI 773 ACATGT [GA]CATG^[CT] PflMI 499 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 471 GAGTC GAGTC(4/5) RsaI 719 GTAC GT^AC SfcI 67 CTACAG C^T[GA][CT]AG SspBI 718 TGTACA T^GTACA TspRI 321 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 499 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 312 CCACCTTTTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 18 GTCTAC GT^[AC][GT]AC 520 GTAGAC AcsI 31 GAATTT [GA]^AATT[CT] 918 AAATTT ApoI 31 GAATTT [GA]^AATT[CT] 918 AAATTT AvaII 153 GGACC G^G[AT]CC 508 GGTCC BalI 347 TGGCCA TGG^CCA 778 TGGCCA BbvI 788 GCAGC GCAGC(8/12) 287 GCTGC BsaJI 59 CCTGGG C^C[ACGT][ACGT]GG 413 CCCAGG BsmFI 410 GTCCC GGGAC(10/14) 509 GTCCC BsrDI 95 CATTGC GCAATG(2/0) 931 GCAATG BsrI 322 ACTGG ACTGG(1/-1) 555 CCAGT EaeI 347 TGGCCA [CT]^GGCC[GA] 778 TGGCCA EcoT22I 460 ATGCAT ATGCA^T 759 ATGCAT Fnu4HI 287 GCTGC GC^[ACGT]GC 788 GCAGC ItaI 287 GCTGC GC^[ACGT]GC 788 GCAGC MluNI 347 TGGCCA TGG^CCA 778 TGGCCA MscI 347 TGGCCA TGG^CCA 778 TGGCCA MseI 644 TTAA T^TAA 923 TTAA NlaIV 153 GGACCC GG[ACGT]^[ACGT]CC 508 GGTCCC NsiI 460 ATGCAT ATGCA^T 759 ATGCAT SfaNI 678 GCATC GCATC(5/9) 761 GCATC TfiI 807 GATTC G^A[AT]TC 870 GAATC Tru9I 644 TTAA T^TAA 923 TTAA TseI 287 GCTGC G^C[AT]GC 788 GCAGC Tsp45I 263 GTCAC ^GT[GC]AC 536 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AluI BsiYI BslI BssKI BstNI Cfr13I DdeI DpnII EcoRII HaeIII HinfI MaeIII MboI MboII MnlI MslI MvaI NdeII NlaIII Sau3AI Sau96I ScrFI Tsp509I