(Circular) (Six-base) MAPSORT of: pFN21AE6092.seq Check: 9867 from: 1 to: 5683 pFN21AE6092 5683 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3493 278 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3026 1937 Size: 1089 4594 Acc65I G'GTAC_C Cuts at: 3004 3004 Size: 5683 AclI AA'CG_TT Cuts at: 1563 3810 4183 1563 Size: 2247 373 3063 Fragments arranged by size: 3063 2247 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3746 5130 5398 1632 Size: 2114 1384 268 1917 Fragments arranged by size: 2114 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5486 AflIII A'CryG_T Cuts at: 1203 2229 4582 5559 1203 Size: 1026 2353 977 1327 Fragments arranged by size: 2353 1327 1026 977 AgeI A'CCGG_T Cuts at: 4514 4514 Size: 5683 AhdI GACnn_n'nnGTC Cuts at: 4412 4412 Size: 5683 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5683 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2487 1184 Size: 1303 4380 AlwNI CAG_nnn'CTG Cuts at: 3426 4998 3426 Size: 1572 4111 ApaLI G'TGCA_C Cuts at: 3740 4896 3740 Size: 1156 4527 ApoI r'AATT_y Cuts at: 1493 2056 2081 2242 2992 3218 3370 5479 1493 Size: 563 25 161 750 226 152 2109 1697 Fragments arranged by size: 2109 1697 750 563 226 161 152 25 AseI AT'TA_AT Cuts at: 160 1032 4237 160 Size: 872 3205 1606 Fragments arranged by size: 3205 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 AvaI C'yCGr_G Cuts at: 1870 1943 3008 1870 Size: 73 1065 4545 Fragments arranged by size: 4545 1065 73 BamHI G'GATC_C Cuts at: 2696 3013 3380 2696 Size: 317 367 4999 Fragments arranged by size: 4999 367 317 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3004 4459 618 Size: 325 217 627 1217 1455 1842 Fragments arranged by size: 1842 1455 1217 627 325 217 BanII G_rGCy'C Cuts at: 1391 2834 3002 1391 Size: 1443 168 4072 Fragments arranged by size: 4072 1443 168 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4712 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3874 3908 1903 Size: 34 1937 34 3678 Fragments arranged by size: 3678 1937 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3575 4785 3575 Size: 1210 4473 BclI T'GATC_A Cuts at: 1548 2410 1548 Size: 862 4821 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4294 136 Size: 107 122 71 631 605 2622 1525 Fragments arranged by size: 2622 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2121 5678 2121 Size: 3557 2126 BlpI GC'TnA_GC Cuts at: 3100 3100 Size: 5683 Bme1580I G_kGCm'C Cuts at: 1790 3744 4900 1790 Size: 1954 1156 2573 Fragments arranged by size: 2573 1954 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5683 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2469 2754 3414 Size: 416 53 83 399 24 1052 285 660 Cuts at: 3414 4372 442 Size: 958 1753 Fragments arranged by size: 1753 1052 958 660 416 399 285 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5683 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2330 2909 4343 1239 Size: 1091 579 1434 2579 Fragments arranged by size: 2579 1434 1091 579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3808 4673 4971 3808 Size: 865 298 4520 Fragments arranged by size: 4520 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2781 4346 882 Size: 617 1282 1565 2219 Fragments arranged by size: 2219 1565 1282 617 BsaAI yAC'GTr Cuts at: 493 1204 3474 493 Size: 711 2270 2702 Fragments arranged by size: 2702 2270 711 BsaBI GATnn'nnATC Cuts at: 5677 5677 Size: 5683 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3490 3872 4507 275 Size: 53 83 186 936 1957 382 635 1451 Fragments arranged by size: 1957 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4116 4514 4788 4935 5346 4116 Size: 398 274 147 411 4453 Fragments arranged by size: 4453 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5458 Fragments arranged by size: 5458 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5683 BseYI C'CCAG_C Cuts at: 2493 4886 5382 2493 Size: 2393 496 2794 Fragments arranged by size: 2794 2393 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5110 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3133 3894 4922 1540 Size: 275 186 1132 761 1028 2301 Fragments arranged by size: 2301 1132 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3744 3829 4900 3744 Size: 85 1071 4527 Fragments arranged by size: 4527 1071 85 BsmI GAATG_Cn' Cuts at: 2387 3194 3287 2387 Size: 807 93 4783 Fragments arranged by size: 4783 807 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2834 3002 3744 3829 4900 1391 Size: 399 1044 168 742 85 1071 2174 Fragments arranged by size: 2174 1071 1044 742 399 168 85 BspEI T'CCGG_A Cuts at: 5346 5346 Size: 5683 BspHI T'CATG_A Cuts at: 2454 3572 5302 2454 Size: 1118 1730 2835 Fragments arranged by size: 2835 1730 1118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2014 3038 844 Size: 1170 1024 3489 Fragments arranged by size: 3489 1170 1024 BsrBI CCG'CTC Cuts at: 1394 1823 3570 1394 Size: 429 1747 3507 Fragments arranged by size: 3507 1747 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2181 4178 4352 65 Size: 1183 933 1997 174 1396 Fragments arranged by size: 1997 1396 1183 933 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4327 4514 1700 Size: 110 2517 187 2869 Fragments arranged by size: 2869 2517 187 110 BsrGI T'GTAC_A Cuts at: 96 2226 96 Size: 2130 3553 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5683 BssSI C'ACGA_G Cuts at: 3743 4755 3743 Size: 1012 4671 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5683 BstBI TT'CG_AA Cuts at: 4487 4487 Size: 5683 BstEII G'GTnAC_C Cuts at: 1184 2890 1184 Size: 1706 3977 BstXI CCAn_nnnn'nTGG Cuts at: 2293 2293 Size: 5683 BstYI r'GATC_y Cuts at: 1972 2121 2696 3013 3380 3765 3782 5234 5320 Size: 149 575 317 367 385 17 1452 86 Cuts at: 5320 5332 5678 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 575 385 367 346 317 149 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2951 2951 Size: 5683 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5131 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4944 Fragments arranged by size: 4944 683 56 BtsI GCAGTG_nn' Cuts at: 3199 3993 4013 3199 Size: 794 20 4869 Fragments arranged by size: 4869 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5683 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5648 DraI TTT'AAA Cuts at: 2987 2987 Size: 5683 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4690 809 Size: 507 3374 1802 Fragments arranged by size: 3374 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3130 4019 5648 Size: 54 1425 50 275 18 1300 889 1629 Cuts at: 5648 8 Size: 43 Fragments arranged by size: 1629 1425 1300 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3130 1537 Size: 275 1318 4090 Fragments arranged by size: 4090 1318 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2135 2278 2906 3613 1353 Size: 782 143 628 707 3423 Fragments arranged by size: 3423 782 707 628 143 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4270 4644 4790 387 Size: 1064 138 2681 374 146 1280 Fragments arranged by size: 2681 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2330 2909 3746 4343 5130 5398 1239 Size: 393 698 579 837 597 787 268 1524 Fragments arranged by size: 1524 837 787 698 597 579 393 268 EcoRI G'AATT_C Cuts at: 2992 3370 2992 Size: 378 5305 FspI TGC'GCA Cuts at: 4189 4189 Size: 5683 HaeII r_GCGC'y Cuts at: 1678 4830 1678 Size: 3152 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2328 2360 2449 2481 4324 Size: 32 135 32 756 32 89 32 1843 Cuts at: 4324 4356 4398 4430 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1843 756 135 89 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2299 3027 3273 669 Size: 988 281 361 728 246 3079 Fragments arranged by size: 3079 988 728 361 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5683 HpaI GTT'AAC Cuts at: 3273 3273 Size: 5683 KpnI G_GTAC'C Cuts at: 3008 3008 Size: 5683 MfeI C'AATT_G Cuts at: 3282 3282 Size: 5683 MluI A'CGCG_T Cuts at: 5559 5559 Size: 5683 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2659 2997 4797 4981 1305 Size: 1354 338 1800 184 2007 Fragments arranged by size: 2007 1800 1354 338 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3861 Fragments arranged by size: 3861 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2222 2228 2380 2469 2825 3641 4000 Size: 648 1056 6 152 89 356 816 359 Cuts at: 4000 4159 518 Size: 159 2042 Fragments arranged by size: 2042 1056 816 648 359 356 159 152 89 6 MspA1I CmG'CkG Cuts at: 1606 3099 3776 4924 5169 1606 Size: 1493 677 1148 245 2120 Fragments arranged by size: 2120 1493 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5131 NdeI CA'TA_TG Cuts at: 387 2152 387 Size: 1765 3918 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5683 NotI GC'GGCC_GC Cuts at: 3130 3130 Size: 5683 NspI r_CATG'y Cuts at: 1132 1229 2233 3041 4586 1132 Size: 97 1004 808 1545 2229 Fragments arranged by size: 2229 1545 1004 808 97 PasI CC'CwG_GG Cuts at: 1348 2591 1348 Size: 1243 4440 PciI A'CATG_T Cuts at: 2229 4582 2229 Size: 2353 3330 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5683 PmeI GTTT'AAAC Cuts at: 2987 2987 Size: 5683 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3752 3784 5265 5297 3752 Size: 32 1481 32 4138 Fragments arranged by size: 4138 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5683 PsiI TTA'TAA Cuts at: 3253 3253 Size: 5683 PstI C_TGCA'G Cuts at: 830 3035 830 Size: 2205 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5683 SalI G'TCGA_C Cuts at: 1936 3025 1936 Size: 1089 4594 SbfI CC_TGCA'GG Cuts at: 3035 3035 Size: 5683 ScaI AGT'ACT Cuts at: 3931 5643 3931 Size: 1712 3971 SfcI C'TryA_G Cuts at: 826 1045 2277 2803 2850 2917 3031 4166 4847 Size: 219 1232 526 47 67 114 1135 681 Cuts at: 4847 5038 826 Size: 191 1471 Fragments arranged by size: 1471 1232 1135 681 526 219 191 114 67 47 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5683 SmaI CCC'GGG Cuts at: 1872 3010 1872 Size: 1138 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3787 4688 4950 820 Size: 197 926 1844 901 262 1553 Fragments arranged by size: 1844 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3474 493 Size: 2981 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5683 SphI G_CATG'C Cuts at: 1132 3041 1132 Size: 1909 3774 SspI AAT'ATT Cuts at: 5 52 3607 5 Size: 47 3555 2081 Fragments arranged by size: 3555 2081 47 StuI AGG'CCT Cuts at: 1517 2759 1517 Size: 1242 4441 StyI C'CwwG_G Cuts at: 513 1065 2092 3122 513 Size: 552 1027 1030 3074 Fragments arranged by size: 3074 1030 1027 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3710 3727 3880 4065 4510 325 Size: 1255 2130 17 153 185 445 1498 Fragments arranged by size: 2130 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2226 3929 5641 Size: 274 80 33 51 1443 249 1703 1712 Cuts at: 5641 96 Size: 138 Fragments arranged by size: 1712 1703 1443 274 249 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5643 5675 5643 Size: 32 5651 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5683 XbaI T'CTAG_A Cuts at: 3019 3019 Size: 5683 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5683 XmaI C'CCGG_G Cuts at: 1870 3008 1870 Size: 1138 4545 XmnI GAAnn'nnTTC Cuts at: 3812 3812 Size: 5683 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3491 276 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI