################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AviII BfrI BsaBI BsiWI BsmBI BspDI BspLU11I BssHII Bst1107I BstBI BstZ17I ClaI CpoI DrdI Eam1105I Eco47III EcoRV EcoT22I FseI FspI HpaI KpnI KspI MamI MluI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SpeI SphI Sse8387I StuI SwaI XbaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccIII 1160 TCCGGA T^CCGGA AcyI 2003 GGCGCC G[GA]^CG[CT]C Alw44I 2167 GTGCAC G^TGCAC ApaLI 2167 GTGCAC G^TGCAC Asp700I 273 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 974 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2183 GGATCC G^GATCC BbeI 2003 GGCGCC GGCGC^C BbrPI 3122 CACGTG CAC^GTG BbvCI 3090 CCTCAGC CCTCAGC(-5/-2) BcgI 231 GCAGTGGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 2801 AGATCT A^GATCT BlpI 3316 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3316 GCTGAGC GC^T[ACGT]AGC BsaHI 2003 GGCGCC G[GA]^CG[CT]C BseAI 1160 TCCGGA T^CCGGA BsiEI 1462 CGGCCG CG[GA][CT]^CG Bsp1407I 146 TGTACA T^GTACA BspEI 1160 TCCGGA T^CCGGA BsrBI 2782 GAGCGG CCGCTC(-3/-3) BsrGI 146 TGTACA T^GTACA BssSI 262 CTCGTG CACGAG(-5/-1) BstAPI 1469 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1183 GGTTACC G^GT[ACGT]ACC BstPI 1183 GGTTACC G^GT[ACGT]ACC CelII 3316 GCTGAGC GC^T[ACGT]AGC DraI 2872 TTTAAA TTT^AAA DraIII 1750 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1462 CGGCCG C^GGCCG EclXI 1462 CGGCCG C^GGCCG Eco52I 1462 CGGCCG C^GGCCG EcoNI 899 CCTGTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1183 GGTTACC G^GT[ACGT]ACC EcoRI 277 GAATTC G^AATTC FauI 3923 CCCGC CCCGC(4/6) HgaI 13 GACGC GACGC(5/10) Hin1I 2003 GGCGCC G[GA]^CG[CT]C HincII 3466 GTCAAC GT[CT]^[GA]AC HindII 3466 GTCAAC GT[CT]^[GA]AC KasI 2003 GGCGCC G^GCGCC MroI 1160 TCCGGA T^CCGGA NaeI 737 GCCGGC GCC^GGC NarI 2003 GGCGCC GG^CGCC NdeI 2275 CATATG CA^TATG NgoMIV 737 GCCGGC G^CCGGC PflFI 974 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3122 CACGTG CAC^GTG PmlI 3122 CACGTG CAC^GTG PshAI 321 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 3319 GAGCTC GAGCT^C SapI 1435 GAAGAGC GCTCTTC(1/4) SfoI 2003 GGCGCC GGC^GCC SnaBI 3938 TACGTA TAC^GTA SspBI 146 TGTACA T^GTACA SspI 2363 AATATT AAT^ATT Tth111I 974 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 273 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 277 GAATTC [GA]^AATT[CT] 1624 GAATTT AocI 712 CCTCAGG CC^T[ACGT]AGG 2188 CCTGAGG ApoI 277 GAATTC [GA]^AATT[CT] 1624 GAATTT AvrII 1779 CCTAGG C^CTAGG 3634 CCTAGG BciVI 3157 GGATAC GTATCC(6/5) 2645 GTATCC BclI 1091 TGATCA T^GATCA 1292 TGATCA BglI 1630 GCCCATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3832 GCCATGGGGGC BlnI 1779 CCTAGG C^CTAGG 3634 CCTAGG BsaAI 3122 CACGTG [CT]AC^GT[GA] 3938 TACGTA BsaWI 1160 TCCGGA [AT]^CCGG[AT] 3602 ACCGGA BsmI 1642 GAATGC GAATGC(1/-1) 1663 GAATGC BsrDI 293 CATTGC GCAATG(2/0) 1694 GCAATG Bsu36I 712 CCTCAGG CC^T[ACGT]AGG 2188 CCTGAGG Eco81I 712 CCTCAGG CC^T[ACGT]AGG 2188 CCTGAGG FbaI 1091 TGATCA T^GATCA 1292 TGATCA HindIII 460 AAGCTT A^AGCTT 2131 AAGCTT MfeI 479 CAATTG C^AATTG 2810 CAATTG MunI 479 CAATTG C^AATTG 2810 CAATTG PaeR7I 1032 CTCGAG C^TCGAG 1619 CTCGAG PpuMI 319 GGGACCC [GA]G^G[AT]CC[CT] 585 GGGTCCT XhoI 1032 CTCGAG C^TCGAG 1619 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccI AccII AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI