(Linear) (Six-base) MAPSORT of: KIEE3459.seq Check: 1762 from: 1 to: 4026 KIEE3459.orf With 182 enzymes: SgfI * February 23, 2012 13:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 12 4026 Size: 12 4014 AccI GT'mk_AC Cuts at: 0 329 791 2819 4026 Size: 329 462 2028 1207 Fragments arranged by size: 2028 1207 462 329 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1209 1357 2774 3518 3584 4026 Size: 1209 148 1417 744 66 442 Fragments arranged by size: 1417 1209 744 442 148 66 AleI CACnn'nnGTG Cuts at: 0 2672 4026 Size: 2672 1354 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1183 1215 4026 Size: 1183 32 2811 Fragments arranged by size: 2811 1183 32 AlwNI CAG_nnn'CTG Cuts at: 0 27 144 545 911 1006 1475 1540 2430 Size: 27 117 401 366 95 469 65 890 Cuts at: 2430 3214 3310 3853 3961 4026 Size: 784 96 543 108 65 Fragments arranged by size: 890 784 543 469 401 366 117 108 96 95 65 65 27 ApaI G_GGCC'C Cuts at: 0 662 1534 1748 1795 4026 Size: 662 872 214 47 2231 Fragments arranged by size: 2231 872 662 214 47 ApaLI G'TGCA_C Cuts at: 0 2167 4026 Size: 2167 1859 ApoI r'AATT_y Cuts at: 0 277 1624 4026 Size: 277 1347 2402 Fragments arranged by size: 2402 1347 277 AvaI C'yCGr_G Cuts at: 0 50 1032 1619 1701 2148 2906 3445 3515 Size: 50 982 587 82 447 758 539 70 Cuts at: 3515 4026 Size: 511 Fragments arranged by size: 982 758 587 539 511 447 82 70 50 AvrII C'CTAG_G Cuts at: 0 1779 3634 4026 Size: 1779 1855 392 Fragments arranged by size: 1855 1779 392 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2905 2938 4026 Size: 2905 33 1088 Fragments arranged by size: 2905 1088 33 BamHI G'GATC_C Cuts at: 0 2183 4026 Size: 2183 1843 BanI G'GyrC_C Cuts at: 0 1783 2003 3230 3523 4026 Size: 1783 220 1227 293 503 Fragments arranged by size: 1783 1227 503 293 220 BanII G_rGCy'C Cuts at: 0 58 662 950 1534 1708 1748 1795 2732 Size: 58 604 288 584 174 40 47 937 Cuts at: 2732 2975 3085 3323 3370 4026 Size: 243 110 238 47 656 Fragments arranged by size: 937 656 604 584 288 243 238 174 110 58 47 47 40 BbeI G_GCGC'C Cuts at: 0 2007 4026 Size: 2007 2019 BbsI GAAGACnn'nnnn_ Cuts at: 0 605 1149 3779 4026 Size: 605 544 2630 247 Fragments arranged by size: 2630 605 544 247 BbvCI CC'TCA_GC Cuts at: 0 3091 4026 Size: 3091 935 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 220 254 4026 Size: 220 34 3772 Fragments arranged by size: 3772 220 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2656 3151 4026 Size: 2656 495 875 Fragments arranged by size: 2656 875 495 BclI T'GATC_A Cuts at: 0 1091 1292 4026 Size: 1091 201 2734 Fragments arranged by size: 2734 1091 201 BglI GCCn_nnn'nGGC Cuts at: 0 1636 3838 4026 Size: 1636 2202 188 Fragments arranged by size: 2202 1636 188 BglII A'GATC_T Cuts at: 0 2801 4026 Size: 2801 1225 BlpI GC'TnA_GC Cuts at: 0 3317 4026 Size: 3317 709 Bme1580I G_kGCm'C Cuts at: 0 662 1534 1684 1731 1748 1795 2171 2415 Size: 662 872 150 47 17 47 376 244 Cuts at: 2415 3235 4026 Size: 820 791 Fragments arranged by size: 872 820 791 662 376 244 150 47 47 17 BmrI ACTGGGnnnn_n' Cuts at: 0 2069 2425 2448 2528 3088 4026 Size: 2069 356 23 80 560 938 Fragments arranged by size: 2069 938 560 356 80 23 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 363 507 2703 3034 3307 3749 4026 Size: 363 144 2196 331 273 442 277 Fragments arranged by size: 2196 442 363 331 277 273 144 Bpu10I CC'TnA_GC Cuts at: 0 705 2125 3091 4026 Size: 705 1420 966 935 Fragments arranged by size: 1420 966 935 705 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 934 2494 2509 2659 3670 4026 Size: 208 726 1560 15 150 1011 356 Fragments arranged by size: 1560 1011 726 356 208 150 15 BsaI GGTCTCn'nnnn_ Cuts at: 0 1103 2903 3547 4026 Size: 1103 1800 644 479 Fragments arranged by size: 1800 1103 644 479 BsaAI yAC'GTr Cuts at: 0 3124 3940 4026 Size: 3124 816 86 Fragments arranged by size: 3124 816 86 BsaHI Gr'CG_yC Cuts at: 0 2004 4026 Size: 2004 2022 BsaWI w'CCGG_w Cuts at: 0 1160 3602 4026 Size: 1160 2442 424 Fragments arranged by size: 2442 1160 424 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 361 391 749 779 842 872 2676 2706 Size: 361 30 358 30 63 30 1804 30 Cuts at: 2706 3319 3349 4026 Size: 613 30 677 Fragments arranged by size: 1804 677 613 361 358 63 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 623 863 1503 1589 3067 3501 3598 3621 Size: 623 240 640 86 1478 434 97 23 Cuts at: 3621 3667 4026 Size: 46 359 Fragments arranged by size: 1478 640 623 434 359 240 97 86 46 23 BseYI C'CCAG_C Cuts at: 0 27 581 1351 1526 1974 2322 3401 3657 Size: 27 554 770 175 448 348 1079 256 Cuts at: 3657 3846 4026 Size: 189 180 Fragments arranged by size: 1079 770 554 448 348 256 189 180 175 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1044 1833 2790 4026 Size: 1044 789 957 1236 Fragments arranged by size: 1236 1044 957 789 BsiEI CG_ry'CG Cuts at: 0 1465 4026 Size: 1465 2561 BsiHKAI G_wGCw'C Cuts at: 0 200 633 1513 1722 2171 3323 4026 Size: 200 433 880 209 449 1152 703 Fragments arranged by size: 1152 880 703 449 433 209 200 BsmI GAATG_Cn' Cuts at: 0 1648 1669 4026 Size: 1648 21 2357 Fragments arranged by size: 2357 1648 21 Bsp1286I G_dGCh'C Cuts at: 0 58 200 633 662 950 1513 1534 1684 Size: 58 142 433 29 288 563 21 150 Cuts at: 1684 1708 1722 1731 1748 1795 2171 2415 2732 Size: 24 14 9 17 47 376 244 317 Cuts at: 2732 2975 3085 3235 3323 3370 4026 Size: 243 110 150 88 47 656 Fragments arranged by size: 656 563 433 376 317 288 244 243 150 150 142 110 88 58 47 47 29 24 21 17 14 9 BspEI T'CCGG_A Cuts at: 0 1160 4026 Size: 1160 2866 BspHI T'CATG_A Cuts at: 0 558 1295 1967 4026 Size: 558 737 672 2059 Fragments arranged by size: 2059 737 672 558 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 12 2579 2774 4026 Size: 12 2567 195 1252 Fragments arranged by size: 2567 1252 195 12 BsrBI CCG'CTC Cuts at: 0 2784 4026 Size: 2784 1242 BsrDI GCAATG_nn' Cuts at: 0 292 1701 4026 Size: 292 1409 2325 Fragments arranged by size: 2325 1409 292 BsrFI r'CCGG_y Cuts at: 0 115 737 1096 4026 Size: 115 622 359 2930 Fragments arranged by size: 2930 622 359 115 BsrGI T'GTAC_A Cuts at: 0 146 4026 Size: 146 3880 BssSI C'ACGA_G Cuts at: 0 262 4026 Size: 262 3764 BstAPI GCAn_nnn'nTGC Cuts at: 0 1475 4026 Size: 1475 2551 BstEII G'GTnAC_C Cuts at: 0 1183 4026 Size: 1183 2843 BstXI CCAn_nnnn'nTGG Cuts at: 0 1071 2627 3078 4026 Size: 1071 1556 451 948 Fragments arranged by size: 1556 1071 948 451 BstYI r'GATC_y Cuts at: 0 1067 1124 2183 2801 4026 Size: 1067 57 1059 618 1225 Fragments arranged by size: 1225 1067 1059 618 57 Bsu36I CC'TnA_GG Cuts at: 0 713 2189 4026 Size: 713 1476 1837 Fragments arranged by size: 1837 1476 713 BtgI C'CryG_G Cuts at: 0 1673 1770 2917 2976 3262 3370 3833 4026 Size: 1673 97 1147 59 286 108 463 193 Fragments arranged by size: 1673 1147 463 286 193 108 97 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 134 591 4026 Size: 134 457 3435 Fragments arranged by size: 3435 457 134 BtsI GCAGTG_nn' Cuts at: 0 86 238 648 1593 1682 3228 3749 4026 Size: 86 152 410 945 89 1546 521 277 Fragments arranged by size: 1546 945 521 410 277 152 89 86 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 735 770 4026 Size: 735 35 3256 Fragments arranged by size: 3256 735 35 DraI TTT'AAA Cuts at: 0 2874 4026 Size: 2874 1152 DraIII CAC_nnn'GTG Cuts at: 0 1755 4026 Size: 1755 2271 EaeI y'GGCC_r Cuts at: 0 266 1204 1462 2099 2282 3118 3373 4026 Size: 266 938 258 637 183 836 255 653 Fragments arranged by size: 938 836 653 637 266 258 255 183 EagI C'GGCC_G Cuts at: 0 1462 4026 Size: 1462 2564 EarI CTCTTCn'nnn_ Cuts at: 0 1430 3452 3863 4026 Size: 1430 2022 411 163 Fragments arranged by size: 2022 1430 411 163 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 3075 4026 Size: 3075 951 EcoICRI GAG'CTC Cuts at: 0 3321 4026 Size: 3321 705 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 363 507 1209 1357 2703 2774 3034 3307 Size: 363 144 702 148 1346 71 260 273 Cuts at: 3307 3518 3584 3749 4026 Size: 211 66 165 277 Fragments arranged by size: 1346 702 363 277 273 260 211 165 148 144 71 66 EcoNI CCTnn'n_nnAGG Cuts at: 0 903 4026 Size: 903 3123 EcoO109I rG'GnC_Cy Cuts at: 0 320 586 709 908 1426 1530 1537 1745 Size: 320 266 123 199 518 104 7 208 Cuts at: 1745 1791 3839 3889 3903 4026 Size: 46 2048 50 14 123 Fragments arranged by size: 2048 518 320 266 208 199 123 123 104 50 46 14 7 EcoRI G'AATT_C Cuts at: 0 277 4026 Size: 277 3749 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 3544 4026 Size: 3544 482 HaeII r_GCGC'y Cuts at: 0 2007 3392 3408 4026 Size: 2007 1385 16 618 Fragments arranged by size: 2007 1385 618 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 412 444 880 912 1433 1465 1565 1597 Size: 412 32 436 32 521 32 100 32 Cuts at: 1597 2168 2182 2200 2214 3319 3351 3667 3699 Size: 571 14 18 14 1105 32 316 32 Cuts at: 3699 4026 Size: 327 Fragments arranged by size: 1105 571 521 436 412 327 316 100 32 32 32 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 0 3468 4026 Size: 3468 558 HindIII A'AGCT_T Cuts at: 0 460 2131 4026 Size: 460 1671 1895 Fragments arranged by size: 1895 1671 460 KasI G'GCGC_C Cuts at: 0 2003 4026 Size: 2003 2023 MfeI C'AATT_G Cuts at: 0 479 2810 4026 Size: 479 2331 1216 Fragments arranged by size: 2331 1216 479 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2821 3084 4026 Size: 2821 263 942 Fragments arranged by size: 2821 942 263 MscI TGG'CCA Cuts at: 0 268 2101 2284 4026 Size: 268 1833 183 1742 Fragments arranged by size: 1833 1742 268 183 MslI CAynn'nnrTG Cuts at: 0 746 2672 2827 2899 3166 3267 3676 4026 Size: 746 1926 155 72 267 101 409 350 Fragments arranged by size: 1926 746 409 350 267 155 101 72 MspA1I CmG'CkG Cuts at: 0 813 866 1763 2427 2595 4026 Size: 813 53 897 664 168 1431 Fragments arranged by size: 1431 897 813 664 168 53 NaeI GCC'GGC Cuts at: 0 739 4026 Size: 739 3287 NarI GG'CG_CC Cuts at: 0 2004 4026 Size: 2004 2022 NcoI C'CATG_G Cuts at: 0 1673 1770 2976 3833 4026 Size: 1673 97 1206 857 193 Fragments arranged by size: 1673 1206 857 193 97 NdeI CA'TA_TG Cuts at: 0 2276 4026 Size: 2276 1750 NgoMIV G'CCGG_C Cuts at: 0 737 4026 Size: 737 3289 NspI r_CATG'y Cuts at: 0 1218 1694 3165 3301 4026 Size: 1218 476 1471 136 725 Fragments arranged by size: 1471 1218 725 476 136 PasI CC'CwG_GG Cuts at: 0 1854 2335 2962 4026 Size: 1854 481 627 1064 Fragments arranged by size: 1854 1064 627 481 PflMI CCAn_nnn'nTGG Cuts at: 0 1026 1540 1674 3154 4026 Size: 1026 514 134 1480 872 Fragments arranged by size: 1480 1026 872 514 134 PfoI T'CCnGG_A Cuts at: 0 588 3323 4026 Size: 588 2735 703 Fragments arranged by size: 2735 703 588 PmlI CAC'GTG Cuts at: 0 3124 4026 Size: 3124 902 PpuMI rG'GwC_Cy Cuts at: 0 320 586 4026 Size: 320 266 3440 Fragments arranged by size: 3440 320 266 PshAI GACnn'nnGTC Cuts at: 0 325 4026 Size: 325 3701 PspOMI G'GGCC_C Cuts at: 0 658 1530 1744 1791 4026 Size: 658 872 214 47 2235 Fragments arranged by size: 2235 872 658 214 47 PspXI vC'TCGA_Gb Cuts at: 0 1032 4026 Size: 1032 2994 PstI C_TGCA'G Cuts at: 0 23 233 2351 3210 3756 4026 Size: 23 210 2118 859 546 270 Fragments arranged by size: 2118 859 546 270 210 23 PvuII CAG'CTG Cuts at: 0 813 866 1763 2427 2595 4026 Size: 813 53 897 664 168 1431 Fragments arranged by size: 1431 897 813 664 168 53 SacI G_AGCT'C Cuts at: 0 3323 4026 Size: 3323 703 SanDI GG'GwC_CC Cuts at: 0 320 4026 Size: 320 3706 SapI GCTCTTCn'nnn_ Cuts at: 0 1430 4026 Size: 1430 2596 SfcI C'TryA_G Cuts at: 0 19 229 2347 3206 3752 3953 4026 Size: 19 210 2118 859 546 201 73 Fragments arranged by size: 2118 859 546 210 201 73 19 SfoI GGC'GCC Cuts at: 0 2005 4026 Size: 2005 2021 SmaI CCC'GGG Cuts at: 0 52 3447 3517 4026 Size: 52 3395 70 509 Fragments arranged by size: 3395 509 70 52 SmlI C'TyrA_G Cuts at: 0 187 949 1032 1619 2473 2524 2638 3649 Size: 187 762 83 587 854 51 114 1011 Cuts at: 3649 4026 Size: 377 Fragments arranged by size: 1011 854 762 587 377 187 114 83 51 SnaBI TAC'GTA Cuts at: 0 3940 4026 Size: 3940 86 SspI AAT'ATT Cuts at: 0 2365 4026 Size: 2365 1661 StyI C'CwwG_G Cuts at: 0 1412 1673 1770 1779 1787 2456 2976 3634 Size: 1412 261 97 9 8 669 520 658 Cuts at: 3634 3833 4004 4026 Size: 199 171 22 Fragments arranged by size: 1412 669 658 520 261 199 171 97 22 9 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 521 1867 2547 3269 4026 Size: 521 1346 680 722 757 Fragments arranged by size: 1346 757 722 680 521 TatI w'GTAC_w Cuts at: 0 146 2445 2465 3668 4026 Size: 146 2299 20 1203 358 Fragments arranged by size: 2299 1203 358 146 20 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1943 1975 2676 2708 4026 Size: 1943 32 701 32 1318 Fragments arranged by size: 1943 1318 701 32 32 Tth111I GACn'n_nGTC Cuts at: 0 977 4026 Size: 977 3049 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 306 331 1738 2292 3181 4026 Size: 306 25 1407 554 889 845 Fragments arranged by size: 1407 889 845 554 306 25 XhoI C'TCGA_G Cuts at: 0 1032 1619 4026 Size: 1032 587 2407 Fragments arranged by size: 2407 1032 587 XmaI C'CCGG_G Cuts at: 0 50 3445 3515 4026 Size: 50 3395 70 511 Fragments arranged by size: 3395 511 70 50 XmnI GAAnn'nnTTC Cuts at: 0 277 4026 Size: 277 3749 Enzymes that do cut: AarI AccI AcuI AleI AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SanDI SapI SfcI SfoI SmaI SmlI SnaBI SspI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AscI AseI AsiSI BfrBI BmgBI BmtI BplI BsaBI BsiWI BsmBI BssHII BstBI BstZ17I ClaI DrdI EcoRV FseI FspI FspAI HpaI KpnI MluI NheI NotI NruI NsiI PacI PciI PmeI PpiI PsiI PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SgfI SgrAI SpeI SphI SrfI StuI SwaI XbaI ZraI