################################## # NO CUT ENZYMES ( 182 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoRV EcoT14I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MspI MunI MvaI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AflIII 98 ACATGT A^C[GA][CT]GT AlwNI 64 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG BalI 365 TGGCCA TGG^CCA BamHI 147 GGATCC G^GATCC BbvCI 55 CCTCAGC CCTCAGC(-5/-2) BfaI 490 CTAG C^TAG BsiYI 309 CCTCTTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 309 CCTCTTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 39 GGGAC GGGAC(10/14) BspLU11I 98 ACATGT A^CATGT BspMI 63 GCAGGT ACCTGC(4/8) BsrDI 536 CATTGC GCAATG(2/0) BsrI 253 ACTGG ACTGG(1/-1) BstAPI 63 GCAGGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraI 927 TTTAAA TTT^AAA DrdI 502 GACTCTAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 365 TGGCCA [CT]^GGCC[GA] EarI 790 GAAGAG CTCTTC(1/4) HaeIII 366 GGCC GG^CC Ksp632I 790 GAAGAG CTCTTC(1/4) MaeI 490 CTAG C^TAG MaeII 271 ACGT A^CGT MluNI 365 TGGCCA TGG^CCA MscI 365 TGGCCA TGG^CCA MslI 93 CACCTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MwoI 63 GCAGGTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 350 CATATG CA^TATG NlaIV 147 GGATCC GG[ACGT]^[ACGT]CC PstI 61 CTGCAG CTGCA^G SfcI 61 CTGCAG C^T[GA][CT]AG TfiI 233 GATTC G^A[AT]TC Tsp45I 500 GTGAC ^GT[GC]AC TspRI 818 CAGTG CA[GC]TG[ACGT][ACGT]^ XbaI 489 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AseI 573 ATTAAT AT^TAAT 802 ATTAAT AsnI 573 ATTAAT AT^TAAT 802 ATTAAT Asp700I 325 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 849 GAATTCTTTC BspHI 398 TCATGA T^CATGA 897 TCATGA BstYI 51 AGATCC [GA]^GATC[CT] 147 GGATCC EcoRI 493 GAATTC G^AATTC 849 GAATTC EcoT22I 278 ATGCAT ATGCA^T 533 ATGCAT MflI 51 AGATCC [GA]^GATC[CT] 147 GGATCC MspA1I 58 CAGCTG C[AC]G^C[GT]G 753 CAGCTG NsiI 278 ATGCAT ATGCA^T 533 ATGCAT NspI 98 ACATGT [GA]CATG^[CT] 280 GCATGT PleI 502 GACTC GAGTC(4/5) 901 GACTC PshBI 573 ATTAAT AT^TAAT 802 ATTAAT PvuII 58 CAGCTG CAG^CTG 753 CAGCTG RcaI 398 TCATGA T^CATGA 897 TCATGA SfaNI 532 GATGC GCATC(5/9) 758 GCATC XhoII 51 AGATCC [GA]^GATC[CT] 147 GGATCC XmnI 325 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 849 GAATTCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AcsI AluI AlwI ApoI BbvI BstF5I DdeI DpnII Fnu4HI FokI HinfI HphI ItaI MaeIII MboI MboII MnlI MseI NdeII NlaIII Sau3AI Tru9I TseI Tsp509I