################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BcnI BfrI BglI BglII BlnI BpmI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsiYI BslI BsmBI BsoBI Bsp1407I BspDI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI Sau96I SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AciI 55 CCGC CCGC(-3/-1) AflIII 720 ACATGT A^C[GA][CT]GT AlwI 407 GGATC GGATC(4/5) AlwNI 405 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 180 CCTCAGG CC^T[ACGT]AGG Asp700I 908 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 577 GAGCTC G[AT]GC[AT]^C BalI 470 TGGCCA TGG^CCA BanII 577 GAGCTC G[GA]GC[CT]^C BbsI 159 GAAGAC GAAGAC(2/6) BbvCI 677 CCTCAGC CCTCAGC(-5/-2) BclI 236 TGATCA T^GATCA BlpI 286 GCTCAGC GC^T[ACGT]AGC BmyI 577 GAGCTC G[AGT]GC[ACT]^C Bpu1102I 286 GCTCAGC GC^T[ACGT]AGC BpuAI 159 GAAGAC GAAGAC(2/6) BsaAI 93 TACGTA [CT]AC^GT[GA] BseRI 714 CTCCTC GAGGAG(10/8) BsgI 281 GTGCAG GTGCAG(16/14) BsiHKAI 577 GAGCTC G[AT]GC[AT]^C BsmAI 733 GTCTC GTCTC(1/5) BsmFI 254 GGGAC GGGAC(10/14) BsmI 242 GCATTC GAATGC(1/-1) Bsp1286I 577 GAGCTC G[AGT]GC[ACT]^C BspLU11I 720 ACATGT A^CATGT BssKI 333 CCTGG ^CC[ACGT]GG BstNI 333 CCTGG CC^[AT]GG BstYI 407 GGATCT [GA]^GATC[CT] Bsu36I 180 CCTCAGG CC^T[ACGT]AGG Cac8I 438 GCTGGC GC[ACGT]^[ACGT]GC CelII 286 GCTCAGC GC^T[ACGT]AGC DrdI 499 GACTCCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 473 CCATGG C^C[GA][CT]GG EaeI 470 TGGCCA [CT]^GGCC[GA] Eco81I 180 CCTCAGG CC^T[ACGT]AGG EcoRII 333 CCTGG ^CC[AT]GG FbaI 236 TGATCA T^GATCA HphI 879 GGTGA GGTGA(8/7) MaeII 94 ACGT A^CGT MflI 407 GGATCT [GA]^GATC[CT] MluNI 470 TGGCCA TGG^CCA MscI 470 TGGCCA TGG^CCA MseI 893 TTAA T^TAA MspA1I 484 CAGCTG C[AC]G^C[GT]G MvaI 333 CCTGG CC^[AT]GG MwoI 277 GCTTGTGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 473 CCATGG C^CATGG NspI 720 ACATGT [GA]CATG^[CT] PstI 61 CTGCAG CTGCA^G PvuII 484 CAGCTG CAG^CTG SacI 577 GAGCTC GAGCT^C ScaI 568 AGTACT AGT^ACT ScrFI 333 CCTGG CC^[ACGT]GG SfaNI 755 GCATC GCATC(5/9) SfcI 61 CTGCAG C^T[GA][CT]AG SnaBI 93 TACGTA TAC^GTA Tru9I 893 TTAA T^TAA XbaI 573 TCTAGA T^CTAGA XhoII 407 GGATCT [GA]^GATC[CT] XmnI 908 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 186 GAATTT [GA]^AATT[CT] 200 AAATTT AfaI 100 GTAC GT^AC 569 GTAC ApoI 186 GAATTT [GA]^AATT[CT] 200 AAATTT BfaI 227 CTAG C^TAG 574 CTAG BspHI 395 TCATGA T^CATGA 581 TCATGA BsrI 112 ACTGG ACTGG(1/-1) 468 ACTGG BstF5I 30 CATCC GGATG(2/0) 897 GGATG DpnII 237 GATC ^GATC 408 GATC FokI 30 CATCC GGATG(9/13) 897 GGATG HaeIII 336 GGCC GG^CC 471 GGCC MaeI 227 CTAG C^TAG 574 CTAG MboI 237 GATC ^GATC 408 GATC MslI 756 CATCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 762 CATGTATGTG NdeII 237 GATC ^GATC 408 GATC PleI 499 GACTC GAGTC(4/5) 529 GACTC RcaI 395 TCATGA T^CATGA 581 TCATGA RsaI 100 GTAC GT^AC 569 GTAC Sau3AI 237 GATC ^GATC 408 GATC TspRI 17 CAGTG CA[GC]TG[ACGT][ACGT]^ 467 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AluI BbvI BsaJI BspMI DdeI EcoT14I Fnu4HI HinfI ItaI MaeIII MboII MnlI NlaIII StyI TfiI TseI Tsp45I Tsp509I