################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaII AviII BalI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FauI FseI FspI HaeII HapII HgaI Hin1I HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI TaqI Tru9I TspRI Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcsI 976 AAATTT [GA]^AATT[CT] AlwNI 628 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 976 AAATTT [GA]^AATT[CT] Asp700I 780 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 139 GAGCTC G[AT]GC[AT]^C AvaI 587 CTCGGG C^[CT]CG[GA]G AvrII 261 CCTAGG C^CTAGG BamHI 7 GGATCC G^GATCC BanII 139 GAGCTC G[GA]GC[CT]^C BclI 320 TGATCA T^GATCA BlnI 261 CCTAGG C^CTAGG BmyI 139 GAGCTC G[AGT]GC[ACT]^C BsiHKAI 139 GAGCTC G[AT]GC[AT]^C BsmAI 630 GAGAC GTCTC(1/5) BsmFI 771 GGGAC GGGAC(10/14) BsoBI 587 CTCGGG C^[CT]CG[GA]G Bsp1286I 139 GAGCTC G[AGT]GC[ACT]^C BspLU11I 728 ACATGT A^CATGT BspMI 793 ACCTGC ACCTGC(4/8) BstYI 7 GGATCC [GA]^GATC[CT] CfoI 581 GCGC GCG^C Cfr13I 369 GGCCC G^G[ACGT]CC EaeI 422 CGGCCA [CT]^GGCC[GA] EarI 154 CTCTTC CTCTTC(1/4) EcoRV 666 GATATC GAT^ATC FbaI 320 TGATCA T^GATCA HhaI 581 GCGC GCG^C HinP1I 581 GCGC G^CGC HincII 595 GTTGAC GT[CT]^[GA]AC HindII 595 GTTGAC GT[CT]^[GA]AC Ksp632I 154 CTCTTC CTCTTC(1/4) MaeII 489 ACGT A^CGT MflI 7 GGATCC [GA]^GATC[CT] NspI 728 ACATGT [GA]CATG^[CT] PstI 795 CTGCAG CTGCA^G SacI 139 GAGCTC GAGCT^C Sau96I 369 GGCCC G^G[ACGT]CC SfaNI 889 GCATC GCATC(5/9) SfcI 795 CTGCAG C^T[GA][CT]AG SspI 994 AATATT AAT^ATT StuI 782 AGGCCT AGG^CCT Tsp509I 977 AATT ^AATT XbaI 981 TCTAGA T^CTAGA XhoII 7 GGATCC [GA]^GATC[CT] XmnI 780 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AciI 438 CCGC CCGC(-3/-1) 421 GCGG AfaI 130 GTAC GT^AC 497 GTAC AflIII 487 ACACGT A^C[GA][CT]GT 728 ACATGT BbvI 797 GCAGC GCAGC(8/12) 530 GCTGC BseRI 151 CTCCTC GAGGAG(10/8) 391 CTCCTC BssKI 417 CCTGG ^CC[ACGT]GG 807 CCTGG BstF5I 890 CATCC GGATG(2/0) 206 GGATG BstNI 417 CCTGG CC^[AT]GG 807 CCTGG Cac8I 118 GCAGGC GC[ACGT]^[ACGT]GC 358 GCAGGC DsaI 65 CCATGG C^C[GA][CT]GG 425 CCATGG EcoRII 417 CCTGG ^CC[AT]GG 807 CCTGG FokI 890 CATCC GGATG(9/13) 206 GGATG HinfI 613 GATTC G^A[ACGT]TC 924 GAATC HphI 70 GGTGA GGTGA(8/7) 804 TCACC MboII 987 GAAGA GAAGA(8/7) 155 TCTTC MvaI 417 CCTGG CC^[AT]GG 807 CCTGG NcoI 65 CCATGG C^CATGG 425 CCATGG NlaIV 7 GGATCC GG[ACGT]^[ACGT]CC 514 GGTTCC RsaI 130 GTAC GT^AC 497 GTAC ScrFI 417 CCTGG CC^[ACGT]GG 807 CCTGG TfiI 613 GATTC G^A[AT]TC 924 GAATC TseI 530 GCTGC G^C[AT]GC 797 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AluI AlwI BfaI BsaJI DdeI DpnII EcoT14I Fnu4HI HaeIII ItaI MaeI MaeIII MboI MnlI MslI MwoI NdeII NlaIII Sau3AI StyI Tsp45I