################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AfaI AflII AflIII AgeI AocI Aor51HI AscI AseI AsnI Asp718I AspI AvaI AvaII AviII AvrII BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HapII HgaI HindIII HinfI HpaI HpaII KpnI KspI MaeI MaeII MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AciI 121 GCGG CCGC(-3/-1) AcsI 43 AAATTT [GA]^AATT[CT] AcyI 407 GGCGCC G[GA]^CG[CT]C AhdI 530 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 392 GTGCAC G^TGCAC AlwNI 73 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 297 GGGCCC GGGCC^C ApaLI 392 GTGCAC G^TGCAC ApoI 43 AAATTT [GA]^AATT[CT] Asp700I 480 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 530 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 392 GTGCAC G[AT]GC[AT]^C BalI 674 TGGCCA TGG^CCA BamHI 493 GGATCC G^GATCC BanI 407 GGCGCC G^G[CT][GA]CC BbeI 407 GGCGCC GGCGC^C BpmI 307 CTGGAG CTGGAG(16/14) BsaHI 407 GGCGCC G[GA]^CG[CT]C BsiHKAI 392 GTGCAC G[AT]GC[AT]^C BsmI 274 GCATTC GAATGC(1/-1) BspHI 662 TCATGA T^CATGA BspMI 376 ACCTGC ACCTGC(4/8) BsrDI 364 GCAATG GCAATG(2/0) BstF5I 336 CATCC GGATG(2/0) BstYI 493 GGATCC [GA]^GATC[CT] DraI 39 TTTAAA TTT^AAA EaeI 674 TGGCCA [CT]^GGCC[GA] Eam1105I 530 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 628 CTCTTC CTCTTC(1/4) EcoNI 78 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 182 CCTTGG C^C[AT][AT]GG FokI 336 CATCC GGATG(9/13) HaeII 407 GGCGCC [GA]GCGC^[CT] Hin1I 407 GGCGCC G[GA]^CG[CT]C HphI 383 TCACC GGTGA(8/7) KasI 407 GGCGCC G^GCGCC Ksp632I 628 CTCTTC CTCTTC(1/4) MaeIII 148 GTCAC ^GT[ACGT]AC MflI 493 GGATCC [GA]^GATC[CT] MluNI 674 TGGCCA TGG^CCA MscI 674 TGGCCA TGG^CCA MseI 40 TTAA T^TAA NarI 407 GGCGCC GG^CGCC NdeI 142 CATATG CA^TATG PstI 125 CTGCAG CTGCA^G RcaI 662 TCATGA T^CATGA SfcI 125 CTGCAG C^T[GA][CT]AG SfoI 407 GGCGCC GGC^GCC StyI 182 CCTTGG C^C[AT][AT]GG TaqI 15 TCGA T^CGA Tru9I 40 TTAA T^TAA Tsp45I 148 GTCAC ^GT[GC]AC Tsp509I 44 AATT ^AATT TthHB8I 15 TCGA T^CGA XcmI 344 CCACTGCTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 493 GGATCC [GA]^GATC[CT] XmnI 480 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AluI 415 AGCT AG^CT 454 AGCT BanII 186 GGGCTC G[GA]GC[CT]^C 297 GGGCCC BbsI 468 GTCTTC GAAGAC(2/6) 649 GTCTTC BpuAI 468 GTCTTC GAAGAC(2/6) 649 GTCTTC BsmFI 255 GGGAC GGGAC(10/14) 528 GGGAC BsrI 252 ACTGG ACTGG(1/-1) 531 ACTGG BstAPI 94 GCAAGTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 394 GCACTCTATGC Cac8I 166 GCTGGC GC[ACGT]^[ACGT]GC 170 GCCTGC CfoI 34 GCGC GCG^C 408 GCGC DraII 130 GGGGCCT [GA]G^G[ACGT]CC[CT] 297 GGGCCCT EcoO109I 130 GGGGCCT [GA]G^G[ACGT]CC[CT] 297 GGGCCCT HhaI 34 GCGC GCG^C 408 GCGC HinP1I 34 GCGC G^CGC 408 GCGC HincII 63 GTCAAC GT[CT]^[GA]AC 325 GTCAAC HindII 63 GTCAAC GT[CT]^[GA]AC 325 GTCAAC MslI 226 CATAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 360 CACGGCAATG SfaNI 31 GATGC GCATC(5/9) 335 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AlwI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII ItaI MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI TspRI