(Circular) (Six-base) MAPSORT of: pFN21AE3822.seq Check: 8514 from: 1 to: 12286 pFN21AE3822 12286 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2230 2230 Size: 12286 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 10096 278 Size: 53 83 186 936 8560 2468 Fragments arranged by size: 8560 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 9081 9331 9629 1937 Size: 7144 250 298 4594 Fragments arranged by size: 7144 4594 298 250 Acc65I G'GTAC_C Cuts at: 9607 9607 Size: 12286 AclI AA'CG_TT Cuts at: 1563 10413 10786 1563 Size: 8850 373 3063 Fragments arranged by size: 8850 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2072 2421 2432 3393 4620 4851 4862 5126 Size: 440 349 11 961 1227 231 11 264 Cuts at: 5126 5408 5417 6303 7257 7281 7865 8148 8583 Size: 282 9 886 954 24 584 283 435 Cuts at: 8583 9245 9465 10349 11733 12001 1632 Size: 662 220 884 1384 268 1917 Fragments arranged by size: 1917 1384 1227 961 954 886 884 662 584 440 435 349 283 282 268 264 231 220 24 11 11 9 AfeI AGC'GCT Cuts at: 2940 2940 Size: 12286 AflII C'TTAA_G Cuts at: 820 1017 4375 5668 820 Size: 197 3358 1293 7438 Fragments arranged by size: 7438 3358 1293 197 AflIII A'CryG_T Cuts at: 1203 2557 6939 9340 11185 12162 1203 Size: 1354 4382 2401 1845 977 1327 Fragments arranged by size: 4382 2401 1845 1354 1327 977 AgeI A'CCGG_T Cuts at: 11117 11117 Size: 12286 AhdI GACnn_n'nnGTC Cuts at: 11015 11015 Size: 12286 AleI CACnn'nnGTG Cuts at: 1166 2257 5315 1166 Size: 1091 3058 8137 Fragments arranged by size: 8137 3058 1091 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 7941 1184 Size: 6757 5529 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5543 5575 5543 Size: 32 12254 AlwNI CAG_nnn'CTG Cuts at: 3817 10029 11601 3817 Size: 6212 1572 4502 Fragments arranged by size: 6212 4502 1572 ApaI G_GGCC'C Cuts at: 4818 4818 Size: 12286 ApaLI G'TGCA_C Cuts at: 8121 9173 10343 11499 8121 Size: 1052 1170 1156 8908 Fragments arranged by size: 8908 1170 1156 1052 ApoI r'AATT_y Cuts at: 1493 2597 2644 2799 3578 3807 3891 4591 4616 Size: 1104 47 155 779 229 84 700 25 Cuts at: 4616 4748 4786 5141 5491 5555 6203 6635 6902 Size: 132 38 355 350 64 648 432 267 Cuts at: 6902 6923 6968 7631 8401 8785 8926 9520 9595 Size: 21 45 663 770 384 141 594 75 Cuts at: 9595 9821 9973 12082 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1104 779 770 700 663 648 594 432 384 355 350 267 229 226 155 152 141 132 84 75 64 47 45 38 25 21 AseI AT'TA_AT Cuts at: 160 1032 2267 6065 7127 8333 10840 160 Size: 872 1235 3798 1062 1206 2507 1606 Fragments arranged by size: 3798 2507 1606 1235 1206 1062 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12286 AvaI C'yCGr_G Cuts at: 1870 1943 9611 1870 Size: 73 7668 4545 Fragments arranged by size: 7668 4545 73 AvrII C'CTAG_G Cuts at: 7657 7657 Size: 12286 BamHI G'GATC_C Cuts at: 9616 9983 9616 Size: 367 11919 BanI G'GyrC_C Cuts at: 618 943 1160 1787 9607 11062 618 Size: 325 217 627 7820 1455 1842 Fragments arranged by size: 7820 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4818 9605 1391 Size: 3427 4787 4072 Fragments arranged by size: 4787 4072 3427 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2420 4035 928 Size: 971 521 1615 9179 Fragments arranged by size: 9179 1615 971 521 BbvCI CC'TCA_GC Cuts at: 6266 6266 Size: 12286 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 10477 10511 1903 Size: 34 8540 34 3678 Fragments arranged by size: 8540 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3565 3801 3830 8031 8561 10178 11388 3565 Size: 236 29 4201 530 1617 1210 4463 Fragments arranged by size: 4463 4201 1617 1210 530 236 29 BclI T'GATC_A Cuts at: 1548 4156 4533 4825 1548 Size: 2608 377 292 9009 Fragments arranged by size: 9009 2608 377 292 BfrBI ATG'CAT Cuts at: 3226 3266 3731 8466 3226 Size: 40 465 4735 7046 Fragments arranged by size: 7046 4735 465 40 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 10897 136 Size: 107 122 71 631 605 9225 1525 Fragments arranged by size: 9225 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2422 2931 6085 6163 7516 12281 2422 Size: 509 3154 78 1353 4765 2427 Fragments arranged by size: 4765 3154 2427 1353 509 78 BlpI GC'TnA_GC Cuts at: 2942 8608 9102 9703 2942 Size: 5666 494 601 5525 Fragments arranged by size: 5666 5525 601 494 Bme1580I G_kGCm'C Cuts at: 1790 3124 4818 8108 8125 8311 8523 9177 10347 Size: 1334 1694 3290 17 186 212 654 1170 Cuts at: 10347 11503 1790 Size: 1156 2573 Fragments arranged by size: 3290 2573 1694 1334 1170 1156 654 212 186 17 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 12286 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6042 7788 10017 Size: 416 53 83 399 24 4625 1746 2229 Cuts at: 10017 10975 442 Size: 958 1753 Fragments arranged by size: 4625 2229 1753 1746 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 12286 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2173 2593 7870 8555 2173 Size: 420 5277 685 5904 Fragments arranged by size: 5904 5277 685 420 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 3530 5072 5351 7412 7629 8232 9312 Size: 902 1389 1542 279 2061 217 603 1080 Cuts at: 9312 10946 1239 Size: 1634 2579 Fragments arranged by size: 2579 2061 1634 1542 1389 1080 902 603 279 217 Bpu10I CC'TnA_GC Cuts at: 5003 6266 5003 Size: 1263 11023 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5401 5471 5734 7163 8346 10411 11276 11574 5401 Size: 70 263 1429 1183 2065 865 298 6113 Fragments arranged by size: 6113 2065 1429 1183 865 298 263 70 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7236 7977 10949 882 Size: 617 5737 741 2972 2219 Fragments arranged by size: 5737 2972 2219 741 617 BsaAI yAC'GTr Cuts at: 493 1204 2142 3100 7936 9341 10077 493 Size: 711 938 958 4836 1405 736 2702 Fragments arranged by size: 4836 2702 1405 958 938 736 711 BsaBI GATnn'nnATC Cuts at: 4209 5702 12280 4209 Size: 1493 6578 4215 Fragments arranged by size: 6578 4215 1493 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 10093 10475 11110 275 Size: 53 83 186 936 8560 382 635 1451 Fragments arranged by size: 8560 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6651 9432 10719 11117 11391 11538 11949 6651 Size: 2781 1287 398 274 147 411 6988 Fragments arranged by size: 6988 2781 1287 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 7695 7725 1178 Size: 30 165 30 6292 30 5739 Fragments arranged by size: 6292 5739 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2417 2597 2948 9309 1186 Size: 1231 180 351 6361 4163 Fragments arranged by size: 6361 4163 1231 351 180 BseYI C'CCAG_C Cuts at: 3117 3190 6766 7817 8509 11489 11985 3117 Size: 73 3576 1051 692 2980 496 3418 Fragments arranged by size: 3576 3418 2980 1051 692 496 73 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2255 2921 3122 3524 1163 Size: 573 519 666 201 402 9925 Fragments arranged by size: 9925 666 573 519 402 201 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 9736 10497 11525 1540 Size: 275 186 7735 761 1028 2301 Fragments arranged by size: 7735 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2154 7183 8125 9177 10347 10432 11503 2154 Size: 5029 942 1052 1170 85 1071 2937 Fragments arranged by size: 5029 2937 1170 1071 1052 942 85 BsmI GAATG_Cn' Cuts at: 2780 2923 3226 3268 4187 4912 5071 6099 6475 Size: 143 303 42 919 725 159 1028 376 Cuts at: 6475 8468 8500 9797 9890 2780 Size: 1993 32 1297 93 5176 Fragments arranged by size: 5176 1993 1297 1028 919 725 376 303 159 143 93 42 32 BsmBI CGTCTCn'nnnn_ Cuts at: 8422 9562 8422 Size: 1140 11146 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 3124 4818 7183 8108 8125 8311 Size: 399 364 970 1694 2365 925 17 186 Cuts at: 8311 8523 9177 9605 10347 10432 11503 1391 Size: 212 654 428 742 85 1071 2174 Fragments arranged by size: 2365 2174 1694 1071 970 925 742 654 428 399 364 212 186 85 17 BspEI T'CCGG_A Cuts at: 11949 11949 Size: 12286 BspHI T'CATG_A Cuts at: 2730 3484 4828 9375 10175 11905 2730 Size: 754 1344 4547 800 1730 3111 Fragments arranged by size: 4547 3111 1730 1344 800 754 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2230 6182 7602 9641 844 Size: 1386 3952 1420 2039 3489 Fragments arranged by size: 3952 3489 2039 1420 1386 BsrBI CCG'CTC Cuts at: 1394 1823 10173 1394 Size: 429 8350 3507 Fragments arranged by size: 8350 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2746 2924 5626 8037 8061 10781 10955 Size: 1183 1498 178 2702 2411 24 2720 174 Cuts at: 10955 65 Size: 1396 Fragments arranged by size: 2720 2702 2411 1498 1396 1183 178 174 24 BsrFI r'CCGG_y Cuts at: 1700 1810 10930 11117 1700 Size: 110 9120 187 2869 Fragments arranged by size: 9120 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3715 96 Size: 3619 8667 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12286 BssSI C'ACGA_G Cuts at: 4302 5074 10346 11358 4302 Size: 772 5272 1012 5230 Fragments arranged by size: 5272 5230 1012 772 BstAPI GCAn_nnn'nTGC Cuts at: 794 6396 8048 794 Size: 5602 1652 5032 Fragments arranged by size: 5602 5032 1652 BstBI TT'CG_AA Cuts at: 11090 11090 Size: 12286 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 12286 BstXI CCAn_nnnn'nTGG Cuts at: 2318 2540 4054 4276 4810 5866 2318 Size: 222 1514 222 534 1056 8738 Fragments arranged by size: 8738 1514 1056 534 222 222 BstYI r'GATC_y Cuts at: 1972 2125 2422 2931 4226 4298 5233 5849 5890 Size: 153 297 509 1295 72 935 616 41 Cuts at: 5890 6085 6163 6540 7256 7516 9316 9616 9983 Size: 195 78 377 716 260 1800 300 367 Cuts at: 9983 10368 10385 11837 11923 11935 12281 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1800 1452 1295 935 716 616 509 385 377 367 346 300 297 260 195 153 86 78 72 41 17 12 Bsu36I CC'TnA_GG Cuts at: 4576 6870 4576 Size: 2294 9992 BtgI C'CryG_G Cuts at: 513 1065 2004 8312 513 Size: 552 939 6308 4487 Fragments arranged by size: 6308 4487 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4599 487 Size: 683 56 3373 8174 Fragments arranged by size: 8174 3373 683 56 BtsI GCAGTG_nn' Cuts at: 3456 3779 6770 9084 9270 9802 10596 10616 3456 Size: 323 2991 2314 186 532 794 20 5126 Fragments arranged by size: 5126 2991 2314 794 532 323 186 20 ClaI AT'CG_AT Cuts at: 1554 7135 1554 Size: 5581 6705 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 12251 DraI TTT'AAA Cuts at: 2650 5406 5583 5929 9590 2650 Size: 2756 177 346 3661 5346 Fragments arranged by size: 5346 3661 2756 346 177 DraIII CAC_nnn'GTG Cuts at: 6466 8002 6466 Size: 1536 10750 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 11293 809 Size: 507 9977 1802 Fragments arranged by size: 9977 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3181 4398 9733 Size: 54 1425 50 275 18 1351 1217 5335 Cuts at: 9733 10622 12251 8 Size: 889 1629 43 Fragments arranged by size: 5335 1629 1425 1351 1217 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 9733 1537 Size: 275 7921 4090 Fragments arranged by size: 7921 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2028 2652 4651 5102 6314 10216 1353 Size: 675 624 1999 451 1212 3902 3423 Fragments arranged by size: 3902 3423 1999 1212 675 624 451 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 10873 11247 11393 387 Size: 1064 138 9284 374 146 1280 Fragments arranged by size: 9284 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2141 2421 2432 3393 3530 4620 Size: 393 440 69 280 11 961 137 1090 Cuts at: 4620 4851 4862 5072 5126 5351 5408 5417 6303 Size: 231 11 210 54 225 57 9 886 Cuts at: 6303 7257 7281 7412 7629 7865 8148 8232 8583 Size: 954 24 131 217 236 283 84 351 Cuts at: 8583 9245 9312 9465 10349 10946 11733 12001 1239 Size: 662 67 153 884 597 787 268 1524 Fragments arranged by size: 1524 1090 961 954 886 884 787 662 597 440 393 351 283 280 268 236 231 225 217 210 153 137 131 84 69 67 57 54 24 11 11 9 EcoNI CCTnn'n_nnAGG Cuts at: 6269 6269 Size: 12286 EcoO109I rG'GnC_Cy Cuts at: 2306 4814 5642 2306 Size: 2508 828 8950 Fragments arranged by size: 8950 2508 828 EcoRI G'AATT_C Cuts at: 2799 6203 8401 9595 9973 2799 Size: 3404 2198 1194 378 5112 Fragments arranged by size: 5112 3404 2198 1194 378 EcoRV GAT'ATC Cuts at: 4541 7803 4541 Size: 3262 9024 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3444 7882 8391 3444 Size: 4438 509 7339 Fragments arranged by size: 7339 4438 509 FspI TGC'GCA Cuts at: 10792 10792 Size: 12286 HaeII r_GCGC'y Cuts at: 1678 2942 3518 11433 1678 Size: 1264 576 7915 2531 Fragments arranged by size: 7915 2531 1264 576 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2407 2439 3728 3760 4525 Size: 32 135 32 835 32 1289 32 765 Cuts at: 4525 4557 4595 4627 5785 5817 6153 6185 6532 Size: 32 38 32 1158 32 336 32 347 Cuts at: 6532 6564 6667 6699 7248 7280 8130 8162 9458 Size: 32 103 32 549 32 850 32 1296 Cuts at: 9458 9490 10927 10959 11001 11033 1373 Size: 32 1437 32 42 32 2626 Fragments arranged by size: 2626 1437 1296 1289 1158 850 835 765 549 347 336 135 103 42 38 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2640 3273 4329 9332 9630 9876 Size: 988 281 702 633 1056 5003 298 246 Cuts at: 9876 669 Size: 3079 Fragments arranged by size: 5003 3079 1056 988 702 633 298 281 246 HindIII A'AGCT_T Cuts at: 748 2296 2550 7239 8846 748 Size: 1548 254 4689 1607 4188 Fragments arranged by size: 4689 4188 1607 1548 254 HpaI GTT'AAC Cuts at: 2640 9876 2640 Size: 7236 5050 KpnI G_GTAC'C Cuts at: 9611 9611 Size: 12286 MfeI C'AATT_G Cuts at: 2351 6630 9885 2351 Size: 4279 3255 4752 Fragments arranged by size: 4752 4279 3255 MluI A'CGCG_T Cuts at: 12162 12162 Size: 12286 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2299 5276 6541 7105 7608 8779 9084 11400 Size: 994 2977 1265 564 503 1171 305 2316 Cuts at: 11400 11584 1305 Size: 184 2007 Fragments arranged by size: 2977 2316 2007 1265 1171 994 564 503 305 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3183 4400 10 Size: 54 1425 343 1351 1217 7896 Fragments arranged by size: 7896 1425 1351 1217 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2257 3076 3221 3333 5064 5315 5731 Size: 648 1091 819 145 112 1731 251 416 Cuts at: 5731 6119 6366 8905 9071 9539 10244 10603 10762 Size: 388 247 2539 166 468 705 359 159 Cuts at: 10762 518 Size: 2042 Fragments arranged by size: 2539 2042 1731 1091 819 705 648 468 416 388 359 251 247 166 159 145 112 MspA1I CmG'CkG Cuts at: 1606 2019 2946 3508 5287 6568 6688 7817 8408 Size: 413 927 562 1779 1281 120 1129 591 Cuts at: 8408 8807 9702 10379 11527 11772 1606 Size: 399 895 677 1148 245 2120 Fragments arranged by size: 2120 1779 1281 1148 1129 927 895 677 591 562 413 399 245 120 NcoI C'CATG_G Cuts at: 513 1065 2004 8312 513 Size: 552 939 6308 4487 Fragments arranged by size: 6308 4487 939 552 NdeI CA'TA_TG Cuts at: 387 4905 387 Size: 4518 7768 NheI G'CTAG_C Cuts at: 1052 1052 Size: 12286 NotI GC'GGCC_GC Cuts at: 9733 9733 Size: 12286 NruI TCG'CGA Cuts at: 2228 2228 Size: 12286 NsiI A_TGCA'T Cuts at: 3228 3268 3733 8468 3228 Size: 40 465 4735 7046 Fragments arranged by size: 7046 4735 465 40 NspI r_CATG'y Cuts at: 1132 1229 2561 3117 3553 6943 7162 9644 11189 Size: 97 1332 556 436 3390 219 2482 1545 Cuts at: 11189 1132 Size: 2229 Fragments arranged by size: 3390 2482 2229 1545 1332 556 436 219 97 PacI TTA_AT'TAA Cuts at: 8337 8337 Size: 12286 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 12286 PciI A'CATG_T Cuts at: 2557 6939 11185 2557 Size: 4382 4246 3658 Fragments arranged by size: 4382 4246 3658 PflMI CCAn_nnn'nTGG Cuts at: 1108 2119 7345 1108 Size: 1011 5226 6049 Fragments arranged by size: 6049 5226 1011 PfoI T'CCnGG_A Cuts at: 2082 2118 9312 2082 Size: 36 7194 5056 Fragments arranged by size: 7194 5056 36 PmeI GTTT'AAAC Cuts at: 9590 9590 Size: 12286 PmlI CAC'GTG Cuts at: 3100 3100 Size: 12286 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 10355 10387 11868 11900 10355 Size: 32 1481 32 10741 Fragments arranged by size: 10741 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2306 5642 2306 Size: 3336 8950 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 12286 PsiI TTA'TAA Cuts at: 2754 6000 8154 9856 2754 Size: 3246 2154 1702 5184 Fragments arranged by size: 5184 3246 2154 1702 PspOMI G'GGCC_C Cuts at: 4814 4814 Size: 12286 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4299 4331 4299 Size: 32 12254 PstI C_TGCA'G Cuts at: 830 3786 6693 6777 9638 830 Size: 2956 2907 84 2861 3478 Fragments arranged by size: 3478 2956 2907 2861 84 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12286 PvuII CAG'CTG Cuts at: 2019 2946 3508 5287 6568 6688 7817 8408 2019 Size: 927 562 1779 1281 120 1129 591 5897 Fragments arranged by size: 5897 1779 1281 1129 927 591 562 120 SalI G'TCGA_C Cuts at: 1936 9330 9628 1936 Size: 7394 298 4594 Fragments arranged by size: 7394 4594 298 SanDI GG'GwC_CC Cuts at: 2306 2306 Size: 12286 SapI GCTCTTCn'nnn_ Cuts at: 6314 6314 Size: 12286 SbfI CC_TGCA'GG Cuts at: 9638 9638 Size: 12286 ScaI AGT'ACT Cuts at: 6214 8072 8896 10534 12246 6214 Size: 1858 824 1638 1712 6254 Fragments arranged by size: 6254 1858 1712 1638 824 SexAI A'CCwGG_T Cuts at: 5638 5638 Size: 12286 SfcI C'TryA_G Cuts at: 826 1045 3782 5238 6208 6689 6773 7913 8077 Size: 219 2737 1456 970 481 84 1140 164 Cuts at: 8077 9634 10769 11450 11641 826 Size: 1557 1135 681 191 1471 Fragments arranged by size: 2737 1557 1471 1456 1140 1135 970 681 481 219 191 164 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12286 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 12286 SmaI CCC'GGG Cuts at: 1872 9613 1872 Size: 7741 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4375 5380 5450 5668 5713 7142 Size: 197 926 2432 1005 70 218 45 1429 Cuts at: 7142 8361 10390 11291 11553 820 Size: 1219 2029 901 262 1553 Fragments arranged by size: 2432 2029 1553 1429 1219 1005 926 901 262 218 197 70 45 SnaBI TAC'GTA Cuts at: 493 10077 493 Size: 9584 2702 SpeI A'CTAG_T Cuts at: 152 6670 152 Size: 6518 5768 SphI G_CATG'C Cuts at: 1132 3117 3553 9644 1132 Size: 1985 436 6091 3774 Fragments arranged by size: 6091 3774 1985 436 SspI AAT'ATT Cuts at: 5 52 2758 4029 4860 7001 7606 8601 10210 Size: 47 2706 1271 831 2141 605 995 1609 Cuts at: 10210 5 Size: 2081 Fragments arranged by size: 2706 2141 2081 1609 1271 995 831 605 47 StuI AGG'CCT Cuts at: 1517 7036 8371 1517 Size: 5519 1335 5432 Fragments arranged by size: 5519 5432 1335 StyI C'CwwG_G Cuts at: 513 1065 2004 2338 4417 4809 6302 6414 7541 Size: 552 939 334 2079 392 1493 112 1127 Cuts at: 7541 7657 7857 7866 8312 8613 9725 513 Size: 116 200 9 446 301 1112 3074 Fragments arranged by size: 3074 2079 1493 1127 1112 939 552 446 392 334 301 200 116 112 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 9355 10313 10330 10483 10668 11113 325 Size: 1255 7775 958 17 153 185 445 1498 Fragments arranged by size: 7775 1498 1255 958 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3715 6212 6789 Size: 274 80 33 51 1443 1738 2497 577 Cuts at: 6789 8070 8894 10532 12244 96 Size: 1281 824 1638 1712 138 Fragments arranged by size: 2497 1738 1712 1638 1443 1281 824 577 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5065 5097 5208 5240 5414 5446 6791 6823 7695 Size: 32 111 32 174 32 1345 32 872 Cuts at: 7695 7727 8005 8037 12246 12278 5065 Size: 32 278 32 4209 32 5073 Fragments arranged by size: 5073 4209 1345 872 278 174 111 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 8662 1316 Size: 7346 4940 XbaI T'CTAG_A Cuts at: 5738 9622 5738 Size: 3884 8402 XcmI CCAnnnn_n'nnnnTGG Cuts at: 7113 7864 7940 7113 Size: 751 76 11459 Fragments arranged by size: 11459 751 76 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 12286 XmaI C'CCGG_G Cuts at: 1870 9611 1870 Size: 7741 4545 XmnI GAAnn'nnTTC Cuts at: 2780 2799 3659 3860 4594 6880 6903 7377 10415 Size: 19 860 201 734 2286 23 474 3038 Cuts at: 10415 2780 Size: 4651 Fragments arranged by size: 4651 3038 2286 860 734 474 201 23 19 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 10094 276 Size: 53 83 186 936 8560 2468 Fragments arranged by size: 8560 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BbeI BsiWI BstZ17I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV PspXI RsrII SacI SacII SfiI SfoI SrfI SwaI