################################## # NO CUT ENZYMES ( 180 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MaeIII MamI MflI MluI MroI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StuI SwaI TaqI Tsp45I TspRI Tth111I TthHB8I Van91I XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AfaI 792 GTAC GT^AC AlwI 104 GGATC GGATC(4/5) Asp700I 69 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanII 657 GGGCTC G[GA]GC[CT]^C BbsI 121 GTCTTC GAAGAC(2/6) BbvCI 363 GCTGAGG CCTCAGC(-5/-2) BbvI 870 GCTGC GCAGC(8/12) BmyI 657 GGGCTC G[AGT]GC[ACT]^C BpmI 669 CTCCAG CTGGAG(16/14) BpuAI 121 GTCTTC GAAGAC(2/6) BsmFI 207 GGGAC GGGAC(10/14) Bsp1286I 657 GGGCTC G[AGT]GC[ACT]^C BssKI 290 CCTGG ^CC[ACGT]GG BssSI 831 CTCGTG CACGAG(-5/-1) BstNI 290 CCTGG CC^[AT]GG DpnII 105 GATC ^GATC DraI 540 TTTAAA TTT^AAA EcoRI 46 GAATTC G^AATTC EcoRII 290 CCTGG ^CC[AT]GG EcoRV 457 GATATC GAT^ATC Fnu4HI 870 GCTGC GC^[ACGT]GC ItaI 870 GCTGC GC^[ACGT]GC MboI 105 GATC ^GATC MfeI 164 CAATTG C^AATTG MunI 164 CAATTG C^AATTG MvaI 290 CCTGG CC^[AT]GG NdeII 105 GATC ^GATC NspI 59 GCATGC [GA]CATG^[CT] PleI 667 GACTC GAGTC(4/5) RsaI 792 GTAC GT^AC Sau3AI 105 GATC ^GATC ScrFI 290 CCTGG CC^[ACGT]GG SfaNI 900 GCATC GCATC(5/9) SphI 59 GCATGC GCATG^C SspI 447 AATATT AAT^ATT TseI 870 GCTGC G^C[AT]GC XbaI 661 TCTAGA T^CTAGA XmnI 69 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 46 GAATTC [GA]^AATT[CT] 636 AAATTT ApoI 46 GAATTC [GA]^AATT[CT] 636 AAATTT BalI 2 TGGCCA TGG^CCA 755 TGGCCA BsaJI 617 CCTTGG C^C[ACGT][ACGT]GG 653 CCTTGG BsiYI 683 CCCATAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 684 CCATAGGAGGG BslI 683 CCCATAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 684 CCATAGGAGGG BsmAI 624 GAGAC GTCTC(1/5) 665 GAGAC BspMI 145 ACCTGC ACCTGC(4/8) 301 ACCTGC BsrI 84 ACTGG ACTGG(1/-1) 563 CCAGT BstF5I 138 CATCC GGATG(2/0) 374 GGATG BstXI 5 CCACCGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 695 CCATGAAAATGG Cfr13I 681 GGCCC G^G[ACGT]CC 692 GGGCC EaeI 2 TGGCCA [CT]^GGCC[GA] 755 TGGCCA EarI 548 CTCTTC CTCTTC(1/4) 724 CTCTTC EcoT14I 617 CCTTGG C^C[AT][AT]GG 653 CCTTGG FokI 138 CATCC GGATG(9/13) 374 GGATG Ksp632I 548 CTCTTC CTCTTC(1/4) 724 CTCTTC MluNI 2 TGGCCA TGG^CCA 755 TGGCCA MscI 2 TGGCCA TGG^CCA 755 TGGCCA MslI 54 CATCAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 696 CATGAAAATG MspA1I 128 CAGCTG C[AC]G^C[GT]G 881 CAGCTG MwoI 814 GCCTTAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 891 GCTACTGTGGC PvuII 128 CAGCTG CAG^CTG 881 CAGCTG Sau96I 681 GGCCC G^G[ACGT]CC 692 GGGCC StyI 617 CCTTGG C^C[AT][AT]GG 653 CCTTGG TfiI 30 GATTC G^A[AT]TC 193 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AluI BfaI Cac8I DdeI HaeIII HinfI HphI MaeI MboII MnlI MseI NlaIII Tru9I Tsp509I