(Linear) (Six-base) MAPSORT of: KIEE0835.seq  Check: 4521  from: 1  to: 942



KIEE0835.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:44  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     154     310     942 
   Size:        154     156     632

  Fragments arranged by size: 

                 632     156     154
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     942 
   Size:        119     823
 
ApoI r'AATT_y

Cuts at:      0      46     636     942 
   Size:         46     590     306

  Fragments arranged by size: 

                 590     306      46
 
BanII G_rGCy'C

Cuts at:      0     661     942 
   Size:        661     281
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     114     942 
   Size:        114     828
 
BbvCI CC'TCA_GC

Cuts at:      0     364     942 
   Size:        364     578
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     654     942 
   Size:        654     288
 
Bpu10I CC'TnA_GC

Cuts at:      0     307     364     942 
   Size:        307      57     578

  Fragments arranged by size: 

                 578     307      57
 
BseYI C'CCAG_C

Cuts at:      0      63     942 
   Size:         63     879
 
Bsp1286I G_dGCh'C

Cuts at:      0     661     942 
   Size:        661     281
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     154     310     942 
   Size:        154     156     632

  Fragments arranged by size: 

                 632     156     154
 
BssSI C'ACGA_G

Cuts at:      0     831     942 
   Size:        831     111
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      12     702     942 
   Size:         12     690     240

  Fragments arranged by size: 

                 690     240      12
 
DraI TTT'AAA

Cuts at:      0     542     942 
   Size:        542     400
 
EaeI y'GGCC_r

Cuts at:      0       2     755     942 
   Size:          2     753     187

  Fragments arranged by size: 

                 753     187       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     554     730     942 
   Size:        554     176     212

  Fragments arranged by size: 

                 554     212     176
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     654     942 
   Size:        119     535     288

  Fragments arranged by size: 

                 535     288     119
 
EcoRI G'AATT_C

Cuts at:      0      46     942 
   Size:         46     896
 
EcoRV GAT'ATC

Cuts at:      0     459     942 
   Size:        459     483
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     517     549     942 
   Size:        517      32     393

  Fragments arranged by size: 

                 517     393      32
 
MfeI C'AATT_G

Cuts at:      0     164     942 
   Size:        164     778
 
MscI TGG'CCA

Cuts at:      0       4     757     942 
   Size:          4     753     185

  Fragments arranged by size: 

                 753     185       4
 
MslI CAynn'nnrTG

Cuts at:      0      58     700     942 
   Size:         58     642     242

  Fragments arranged by size: 

                 642     242      58
 
MspA1I CmG'CkG

Cuts at:      0     130     883     942 
   Size:        130     753      59

  Fragments arranged by size: 

                 753     130      59
 
NspI r_CATG'y

Cuts at:      0      63     942 
   Size:         63     879
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     775     807     942 
   Size:        775      32     135

  Fragments arranged by size: 

                 775     135      32
 
PvuII CAG'CTG

Cuts at:      0     130     883     942 
   Size:        130     753      59

  Fragments arranged by size: 

                 753     130      59
 
SphI G_CATG'C

Cuts at:      0      63     942 
   Size:         63     879
 
SspI AAT'ATT

Cuts at:      0     449     942 
   Size:        449     493
 
StyI C'CwwG_G

Cuts at:      0     617     653     942 
   Size:        617      36     289

  Fragments arranged by size: 

                 617     289      36
 
TatI w'GTAC_w

Cuts at:      0     791     942 
   Size:        791     151
 
XbaI T'CTAG_A

Cuts at:      0     661     942 
   Size:        661     281
 
XmnI GAAnn'nnTTC

Cuts at:      0      73     942 
   Size:         73     869
 

 Enzymes that do cut:

     AarI     AcuI     ApoI    BanII     BbsI    BbvCI     BpmI   Bpu10I
    BseYI Bsp1286I    BspMI    BssSI    BstXI     DraI     EaeI     EarI
  Eco57MI    EcoRI    EcoRV    Hin4I     MfeI     MscI     MslI   MspA1I
     NspI     PsrI    PvuII     SphI     SspI     StyI     TatI     XbaI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI EcoO109I     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     StuI     SwaI    TaqII     TstI  Tth111I     XcmI
     XhoI     XmaI     ZraI