################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AocI 897 CCTGAGG CC^T[ACGT]AGG AseI 858 ATTAAT AT^TAAT AsnI 858 ATTAAT AT^TAAT AspHI 553 GAGCTC G[AT]GC[AT]^C AvaI 799 CTCGAG C^[CT]CG[GA]G AvaII 698 GGTCC G^G[AT]CC BalI 368 TGGCCA TGG^CCA BamHI 68 GGATCC G^GATCC BanI 436 GGTGCC G^G[CT][GA]CC BanII 553 GAGCTC G[GA]GC[CT]^C BbvI 4 GCAGC GCAGC(8/12) BcnI 58 CCGGG CC^[GC]GG BmyI 553 GAGCTC G[AGT]GC[ACT]^C BpmI 663 CTCCAG CTGGAG(16/14) BsaAI 364 TACGTG [CT]AC^GT[GA] BseRI 900 GAGGAG GAGGAG(10/8) BsgI 160 CTGCAC GTGCAG(16/14) BsiHKAI 553 GAGCTC G[AT]GC[AT]^C BsmBI 321 CGTCTC CGTCTC(1/5) BsoBI 799 CTCGAG C^[CT]CG[GA]G Bsp1286I 553 GAGCTC G[AGT]GC[ACT]^C Bsp1407I 389 TGTACA T^GTACA BsrGI 389 TGTACA T^GTACA BstEII 105 GGTCACC G^GT[ACGT]ACC BstPI 105 GGTCACC G^GT[ACGT]ACC BstYI 68 GGATCC [GA]^GATC[CT] Bsu36I 897 CCTGAGG CC^T[ACGT]AGG DpnII 69 GATC ^GATC DraIII 279 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 508 GACAACCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 368 TGGCCA [CT]^GGCC[GA] Eco81I 897 CCTGAGG CC^T[ACGT]AGG EcoO65I 105 GGTCACC G^GT[ACGT]ACC EcoT14I 464 CCATGG C^C[AT][AT]GG Fnu4HI 4 GCAGC GC^[ACGT]GC HapII 58 CCGG C^CGG HincII 610 GTTGAC GT[CT]^[GA]AC HindII 610 GTTGAC GT[CT]^[GA]AC HpaII 58 CCGG C^CGG ItaI 4 GCAGC GC^[ACGT]GC MboI 69 GATC ^GATC MflI 68 GGATCC [GA]^GATC[CT] MluNI 368 TGGCCA TGG^CCA MscI 368 TGGCCA TGG^CCA MspI 58 CCGG C^CGG NciI 58 CCGGG CC^[GC]GG NcoI 464 CCATGG C^CATGG NdeII 69 GATC ^GATC PaeR7I 799 CTCGAG C^TCGAG PshBI 858 ATTAAT AT^TAAT SacI 553 GAGCTC GAGCT^C SapI 297 GCTCTTC GCTCTTC(1/4) Sau3AI 69 GATC ^GATC ScaI 712 AGTACT AGT^ACT SspBI 389 TGTACA T^GTACA StyI 464 CCATGG C^C[AT][AT]GG TseI 4 GCAGC G^C[AT]GC Tsp509I 732 AATT ^AATT XhoI 799 CTCGAG C^TCGAG XhoII 68 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 360 CCGC CCGC(-3/-1) 66 GCGG AlwI 69 GATCC GGATC(4/5) 68 GGATC BsmAI 253 GTCTC GTCTC(1/5) 322 GTCTC BsmFI 60 GGGAC GGGAC(10/14) 841 GTCCC BsmI 620 GAATGC GAATGC(1/-1) 668 GCATTC BspMI 158 ACCTGC ACCTGC(4/8) 695 GCAGGT BstNI 123 CCTGG CC^[AT]GG 411 CCAGG DsaI 110 CCGTGG C^C[GA][CT]GG 464 CCATGG EcoRII 123 CCTGG ^CC[AT]GG 411 CCAGG HinfI 291 GACTC G^A[ACGT]TC 328 GAGTC MaeII 365 ACGT A^CGT 614 ACGT MslI 108 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 165 CACTCCCATG MvaI 123 CCTGG CC^[AT]GG 411 CCAGG MwoI 57 GCCGGGACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 795 GCCCCTCGAGC PleI 291 GACTC GAGTC(4/5) 328 GAGTC SfcI 686 CTACAG C^T[GA][CT]AG 864 CTATAG TaqI 38 TCGA T^CGA 800 TCGA TspRI 383 CACTG CA[GC]TG[ACGT][ACGT]^ 633 CACTG TthHB8I 38 TCGA T^CGA 800 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AfaI AluI BsaJI BsiYI BslI BssKI BstF5I Cfr13I DdeI EarI FokI HaeIII HphI Ksp632I MaeIII MboII MnlI MseI NlaIII NlaIV RsaI Sau96I ScrFI SmlI Tru9I Tsp45I