################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstNI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRII EcoRV EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KspI MaeII MamI MfeI MluI MroI MunI MvaI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 624 GGTACC G^GTACC AciI 763 GCGG CCGC(-3/-1) AflIII 338 ACATGT A^C[GA][CT]GT AlwI 793 GGATC GGATC(4/5) Asp718I 624 GGTACC G^GTACC BalI 371 TGGCCA TGG^CCA BbvI 439 GCTGC GCAGC(8/12) BclI 134 TGATCA T^GATCA BcnI 479 CCGGG CC^[GC]GG BmyI 841 GTGCCC G[AGT]GC[ACT]^C BsgI 564 CTGCAC GTGCAG(16/14) BsmAI 744 GTCTC GTCTC(1/5) BsmFI 408 GTCCC GGGAC(10/14) Bsp1286I 841 GTGCCC G[AGT]GC[ACT]^C BspLU11I 338 ACATGT A^CATGT BsrI 629 CCAGT ACTGG(1/-1) BssKI 479 CCGGG ^CC[ACGT]GG BstEII 399 GGTCACC G^GT[ACGT]ACC BstPI 399 GGTCACC G^GT[ACGT]ACC BstYI 793 GGATCT [GA]^GATC[CT] Cfr13I 471 GGCCC G^G[ACGT]CC DraIII 477 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 371 TGGCCA [CT]^GGCC[GA] EarI 187 CTCTTC CTCTTC(1/4) EcoO65I 399 GGTCACC G^GT[ACGT]ACC FbaI 134 TGATCA T^GATCA KpnI 624 GGTACC GGTAC^C Ksp632I 187 CTCTTC CTCTTC(1/4) MflI 793 GGATCT [GA]^GATC[CT] MluNI 371 TGGCCA TGG^CCA MscI 371 TGGCCA TGG^CCA MspA1I 761 CAGCGG C[AC]G^C[GT]G MwoI 122 GCAACCTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 479 CCGGG CC^[GC]GG NcoI 113 CCATGG C^CATGG NdeI 356 CATATG CA^TATG PstI 504 CTGCAG CTGCA^G Sau96I 471 GGCCC G^G[ACGT]CC ScaI 715 AGTACT AGT^ACT ScrFI 479 CCGGG CC^[ACGT]GG SmlI 692 CTCAAG C^T[CT][GA]AG SphI 485 GCATGC GCATG^C TseI 439 GCTGC G^C[AT]GC XhoII 793 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Asp700I 31 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 809 GAAAAGTTTC BanI 624 GGTACC G^G[CT][GA]CC 840 GGTGCC BpmI 553 CTCCAG CTGGAG(16/14) 461 CTGGAG BsiYI 278 CCAATGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 473 CCCACACCGGG BslI 278 CCAATGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 473 CCCACACCGGG BsmI 671 GCATTC GAATGC(1/-1) 766 GCATTC BsrDI 386 CATTGC GCAATG(2/0) 735 CATTGC Cac8I 3 GCTGGC GC[ACGT]^[ACGT]GC 485 GCATGC DsaI 113 CCATGG C^C[GA][CT]GG 467 CCACGG EcoT14I 113 CCATGG C^C[AT][AT]GG 126 CCTTGG Fnu4HI 439 GCTGC GC^[ACGT]GC 763 GCGGC HaeIII 372 GGCC GG^CC 471 GGCC HapII 69 CCGG C^CGG 479 CCGG HincII 342 GTTGAC GT[CT]^[GA]AC 580 GTCAAC HindII 342 GTTGAC GT[CT]^[GA]AC 580 GTCAAC HinfI 294 GACTC G^A[ACGT]TC 494 GACTC HpaII 69 CCGG C^CGG 479 CCGG ItaI 439 GCTGC GC^[ACGT]GC 763 GCGGC MspI 69 CCGG C^CGG 479 CCGG NspI 338 ACATGT [GA]CATG^[CT] 485 GCATGC PleI 294 GACTC GAGTC(4/5) 494 GACTC SfcI 504 CTGCAG C^T[GA][CT]AG 864 CTACAG SspI 268 AATATT AAT^ATT 782 AATATT StyI 113 CCATGG C^C[AT][AT]GG 126 CCTTGG TspRI 507 CAGTG CA[GC]TG[ACGT][ACGT]^ 896 CACTG XmnI 31 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 809 GAAAAGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AcsI AfaI AluI ApoI BfaI BsaJI BseRI BstF5I DdeI DpnII FokI HphI MaeI MaeIII MboI MboII MnlI MseI MslI NdeII NlaIII NlaIV RsaI Sau3AI Tru9I Tsp45I Tsp509I