(Circular) (Six-base) MAPSORT of: pFN21AE5986.seq Check: 6220 from: 1 to: 5644 pFN21AE5986 5644 bp With 182 enzymes: SgfI * December 8, 2014 13:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2987 1937 Size: 1050 4594 Acc65I G'GTAC_C Cuts at: 2629 2965 2629 Size: 336 5308 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2701 3707 5091 5359 1632 Size: 1069 1006 1384 268 1917 Fragments arranged by size: 1917 1384 1069 1006 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 2343 4543 5520 1203 Size: 1140 2200 977 1327 Fragments arranged by size: 2200 1327 1140 977 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 4373 4373 Size: 5644 AleI CACnn'nnGTG Cuts at: 1166 2840 1166 Size: 1674 3970 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5644 AlwNI CAG_nnn'CTG Cuts at: 3387 4959 3387 Size: 1572 4072 ApaLI G'TGCA_C Cuts at: 3701 4857 3701 Size: 1156 4488 ApoI r'AATT_y Cuts at: 1493 2036 2158 2922 2953 3179 3331 5440 1493 Size: 543 122 764 31 226 152 2109 1697 Fragments arranged by size: 2109 1697 764 543 226 152 122 31 AseI AT'TA_AT Cuts at: 160 1032 4198 160 Size: 872 3166 1606 Fragments arranged by size: 3166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2969 1870 Size: 73 1026 4545 Fragments arranged by size: 4545 1026 73 BamHI G'GATC_C Cuts at: 2974 3341 2974 Size: 367 5277 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2629 2845 2965 4420 618 Size: 325 217 627 842 216 120 1455 1842 Fragments arranged by size: 1842 1455 842 627 325 217 216 120 BanII G_rGCy'C Cuts at: 1391 2963 1391 Size: 1572 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4673 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3835 3869 1903 Size: 34 50 34 1814 34 3678 Fragments arranged by size: 3678 1814 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3536 4746 3536 Size: 1210 4434 BclI T'GATC_A Cuts at: 1548 2139 1548 Size: 591 5053 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4255 136 Size: 107 122 71 631 605 337 2246 1525 Fragments arranged by size: 2246 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5639 5639 Size: 5644 BlpI GC'TnA_GC Cuts at: 3061 3061 Size: 5644 Bme1580I G_kGCm'C Cuts at: 1790 2850 3705 4861 1790 Size: 1060 855 1156 2573 Fragments arranged by size: 2573 1156 1060 855 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2628 3375 4333 Size: 416 53 83 399 24 1211 747 958 Cuts at: 4333 442 Size: 1753 Fragments arranged by size: 1753 1211 958 747 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5644 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2928 2928 Size: 5644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2487 2543 4304 1239 Size: 1248 56 1761 2579 Fragments arranged by size: 2579 1761 1248 56 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2682 3769 4634 4932 2682 Size: 1087 865 298 3394 Fragments arranged by size: 3394 1087 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4307 882 Size: 617 2808 2219 Fragments arranged by size: 2808 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3435 493 Size: 711 2231 2702 Fragments arranged by size: 2702 2231 711 BsaBI GATnn'nnATC Cuts at: 5638 5638 Size: 5644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3451 3833 4468 275 Size: 53 83 186 936 1918 382 635 1451 Fragments arranged by size: 1918 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4077 4475 4749 4896 5307 4077 Size: 398 274 147 411 4414 Fragments arranged by size: 4414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2538 2568 1178 Size: 30 165 30 1135 30 4254 Fragments arranged by size: 4254 1135 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2215 2546 2944 1186 Size: 1029 331 398 3886 Fragments arranged by size: 3886 1029 398 331 BseYI C'CCAG_C Cuts at: 4847 5343 4847 Size: 496 5148 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2554 1163 Size: 573 818 4253 Fragments arranged by size: 4253 818 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3094 3855 4883 1540 Size: 275 186 1093 761 1028 2301 Fragments arranged by size: 2301 1093 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3705 3790 4861 3705 Size: 85 1071 4488 Fragments arranged by size: 4488 1071 85 BsmI GAATG_Cn' Cuts at: 2676 2771 3155 3248 2676 Size: 95 384 93 5072 Fragments arranged by size: 5072 384 95 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2850 2963 3705 3790 4861 1391 Size: 399 1060 113 742 85 1071 2174 Fragments arranged by size: 2174 1071 1060 742 399 113 85 BspEI T'CCGG_A Cuts at: 5307 5307 Size: 5644 BspHI T'CATG_A Cuts at: 3533 5263 3533 Size: 1730 3914 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2999 844 Size: 2155 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3531 1394 Size: 429 1708 3507 Fragments arranged by size: 3507 1708 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2390 2739 4139 4313 65 Size: 1183 1142 349 1400 174 1396 Fragments arranged by size: 1400 1396 1183 1142 349 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4288 4475 1700 Size: 110 2478 187 2869 Fragments arranged by size: 2869 2478 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5644 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5644 BssSI C'ACGA_G Cuts at: 3704 4716 3704 Size: 1012 4632 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5644 BstBI TT'CG_AA Cuts at: 4448 4448 Size: 5644 BstEII G'GTnAC_C Cuts at: 1184 2404 1184 Size: 1220 4424 BstYI r'GATC_y Cuts at: 1972 2798 2974 3341 3726 3743 5195 5281 5293 Size: 826 176 367 385 17 1452 86 12 Cuts at: 5293 5639 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 826 385 367 346 176 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2118 2472 513 Size: 552 1053 354 3685 Fragments arranged by size: 3685 1053 552 354 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4905 Fragments arranged by size: 4905 683 56 BtsI GCAGTG_nn' Cuts at: 2518 3160 3954 3974 2518 Size: 642 794 20 4188 Fragments arranged by size: 4188 794 642 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5609 DraI TTT'AAA Cuts at: 2948 2948 Size: 5644 DraIII CAC_nnn'GTG Cuts at: 2487 2487 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4651 809 Size: 507 3335 1802 Fragments arranged by size: 3335 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2376 3091 3980 Size: 54 1425 50 275 18 546 715 889 Cuts at: 3980 5609 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 715 546 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3091 1537 Size: 275 1279 4090 Fragments arranged by size: 4090 1279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2198 3574 1353 Size: 845 1376 3423 Fragments arranged by size: 3423 1376 845 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4231 4605 4751 387 Size: 1064 138 2642 374 146 1280 Fragments arranged by size: 2642 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2487 2543 2701 3707 4304 5091 5359 Size: 393 855 56 158 1006 597 787 268 Cuts at: 5359 1239 Size: 1524 Fragments arranged by size: 1524 1006 855 787 597 393 268 158 56 EcoRI G'AATT_C Cuts at: 2953 3331 2953 Size: 378 5266 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 4791 1678 Size: 3113 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4285 4317 4359 4391 1373 Size: 32 135 32 2713 32 42 32 2626 Fragments arranged by size: 2713 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2349 2587 2988 3234 669 Size: 988 281 411 238 401 246 3079 Fragments arranged by size: 3079 988 411 401 281 246 238 HindIII A'AGCT_T Cuts at: 748 748 Size: 5644 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KpnI G_GTAC'C Cuts at: 2633 2969 2633 Size: 336 5308 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4758 4942 1305 Size: 3453 184 2007 Fragments arranged by size: 3453 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2378 10 Size: 54 1425 343 546 3276 Fragments arranged by size: 3276 1425 546 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 2609 2840 3602 3961 4120 518 Size: 648 1011 432 231 762 359 159 2042 Fragments arranged by size: 2042 1011 762 648 432 359 231 159 MspA1I CmG'CkG Cuts at: 1606 2768 3060 3737 4885 5130 1606 Size: 1162 292 677 1148 245 2120 Fragments arranged by size: 2120 1162 1148 677 292 245 NcoI C'CATG_G Cuts at: 513 1065 2118 513 Size: 552 1053 4039 Fragments arranged by size: 4039 1053 552 NdeI CA'TA_TG Cuts at: 387 2362 387 Size: 1975 3669 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5644 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NspI r_CATG'y Cuts at: 1132 1229 2347 2494 3002 4547 1132 Size: 97 1118 147 508 1545 2229 Fragments arranged by size: 2229 1545 1118 508 147 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5644 PciI A'CATG_T Cuts at: 2343 4543 2343 Size: 2200 3444 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5644 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3745 5226 5258 3713 Size: 32 1481 32 4099 Fragments arranged by size: 4099 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5644 PsiI TTA'TAA Cuts at: 3214 3214 Size: 5644 PstI C_TGCA'G Cuts at: 830 2513 2996 830 Size: 1683 483 3478 Fragments arranged by size: 3478 1683 483 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5644 SalI G'TCGA_C Cuts at: 1936 2986 1936 Size: 1050 4594 SbfI CC_TGCA'GG Cuts at: 2996 2996 Size: 5644 ScaI AGT'ACT Cuts at: 2722 3892 5604 2722 Size: 1170 1712 2762 Fragments arranged by size: 2762 1712 1170 SfcI C'TryA_G Cuts at: 826 1045 2509 2869 2992 4127 4808 4999 826 Size: 219 1464 360 123 1135 681 191 1471 Fragments arranged by size: 1471 1464 1135 681 360 219 191 123 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2971 1872 Size: 1099 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2697 3748 4649 4911 820 Size: 197 926 754 1051 901 262 1553 Fragments arranged by size: 1553 1051 926 901 754 262 197 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 2494 3002 1132 Size: 1362 508 3774 Fragments arranged by size: 3774 1362 508 SspI AAT'ATT Cuts at: 5 52 2275 2789 3568 5 Size: 47 2223 514 779 2081 Fragments arranged by size: 2223 2081 779 514 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5644 StyI C'CwwG_G Cuts at: 513 1065 2118 2131 3083 513 Size: 552 1053 13 952 3074 Fragments arranged by size: 3074 1053 952 552 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2141 2280 3671 3688 3841 4026 4471 Size: 1255 561 139 1391 17 153 185 445 Cuts at: 4471 325 Size: 1498 Fragments arranged by size: 1498 1391 1255 561 445 185 153 139 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2720 3890 Size: 274 80 33 51 1443 204 539 1170 Cuts at: 3890 5602 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1170 539 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5604 5636 5604 Size: 32 5612 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5644 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5644 XmaI C'CCGG_G Cuts at: 1870 2969 1870 Size: 1099 4545 XmnI GAAnn'nnTTC Cuts at: 2040 2818 3773 2040 Size: 778 955 3911 Fragments arranged by size: 3911 955 778 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI