################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstYI CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV FbaI Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI TseI TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 442 GTATAC GT^[AC][GT]AC AluI 724 AGCT AG^CT AocI 873 CCTGAGG CC^T[ACGT]AGG AspI 555 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 639 GGTCC G^G[AT]CC BbsI 819 GTCTTC GAAGAC(2/6) BciVI 671 GTATCC GTATCC(6/5) BpuAI 819 GTCTTC GAAGAC(2/6) BsaI 509 GGTCTC GGTCTC(1/5) BsmAI 510 GTCTC GTCTC(1/5) BsmFI 408 GTCCC GGGAC(10/14) BsmI 286 GAATGC GAATGC(1/-1) Bst1107I 442 GTATAC GTA^TAC BstXI 425 CCATAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 442 GTATAC GTA^TAC Bsu36I 873 CCTGAGG CC^T[ACGT]AGG Cac8I 43 GCAGGC GC[ACGT]^[ACGT]GC DsaI 350 CCATGG C^C[GA][CT]GG EarI 79 CTCTTC CTCTTC(1/4) Eco81I 873 CCTGAGG CC^T[ACGT]AGG EcoT22I 288 ATGCAT ATGCA^T FauI 482 CCCGC CCCGC(4/6) HaeIII 465 GGCC GG^CC HphI 246 GGTGA GGTGA(8/7) Ksp632I 79 CTCTTC CTCTTC(1/4) MaeIII 109 GTCAC ^GT[ACGT]AC MwoI 716 GCACTTGTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 350 CCATGG C^CATGG NlaIV 122 GGAACC GG[ACGT]^[ACGT]CC NsiI 288 ATGCAT ATGCA^T NspI 485 GCATGT [GA]CATG^[CT] PflFI 555 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 729 CCATCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 555 GACTC GAGTC(4/5) SspI 397 AATATT AAT^ATT Tsp45I 109 GTCAC ^GT[GC]AC Tth111I 555 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 729 CCATCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 791 CCAGCACTACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AciI 483 CCGC CCGC(-3/-1) 637 GCGG AlwNI 23 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG 414 CAGGGTCTG BfaI 668 CTAG C^TAG 914 CTAG BpmI 170 CTCCAG CTGGAG(16/14) 567 CTCCAG BsiYI 689 CCACTGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 729 CCATCTCTTGG BslI 689 CCACTGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 729 CCATCTCTTGG BssKI 39 CCTGG ^CC[ACGT]GG 126 CCTGG BstF5I 929 CATCC GGATG(2/0) 885 GGATG BstNI 39 CCTGG CC^[AT]GG 126 CCTGG Cfr13I 464 GGGCC G^G[ACGT]CC 639 GGTCC DpnII 135 GATC ^GATC 208 GATC EcoRII 39 CCTGG ^CC[AT]GG 126 CCTGG FokI 929 CATCC GGATG(9/13) 885 GGATG MaeI 668 CTAG C^TAG 914 CTAG MboI 135 GATC ^GATC 208 GATC MseI 142 TTAA T^TAA 614 TTAA MvaI 39 CCTGG CC^[AT]GG 126 CCTGG NdeII 135 GATC ^GATC 208 GATC Sau3AI 135 GATC ^GATC 208 GATC Sau96I 464 GGGCC G^G[ACGT]CC 639 GGTCC ScrFI 39 CCTGG CC^[ACGT]GG 126 CCTGG SfaNI 240 GATGC GCATC(5/9) 746 GCATC SfcI 504 CTGTAG C^T[GA][CT]AG 867 CTATAG TfiI 588 GATTC G^A[AT]TC 855 GAATC Tru9I 142 TTAA T^TAA 614 TTAA Tsp509I 144 AATT ^AATT 324 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### BsaJI BseRI BsrI DdeI EcoT14I HinfI MboII MnlI MslI NlaIII StyI TspRI