(Circular) (Six-base) MAPSORT of: pFN21AE5931.seq Check: 9320 from: 1 to: 5638 pFN21AE5931 5638 bp With 182 enzymes: SgfI * December 8, 2014 13:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3448 278 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2448 2981 1937 Size: 511 533 4594 Fragments arranged by size: 4594 533 511 Acc65I G'GTAC_C Cuts at: 2959 2959 Size: 5638 AclI AA'CG_TT Cuts at: 1563 3765 4138 1563 Size: 2202 373 3063 Fragments arranged by size: 3063 2202 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2010 2927 3701 5085 5353 1632 Size: 378 917 774 1384 268 1917 Fragments arranged by size: 1917 1384 917 774 378 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5441 AflIII A'CryG_T Cuts at: 1203 4537 5514 1203 Size: 3334 977 1327 Fragments arranged by size: 3334 1327 977 AgeI A'CCGG_T Cuts at: 4469 4469 Size: 5638 AhdI GACnn_n'nnGTC Cuts at: 4367 4367 Size: 5638 AleI CACnn'nnGTG Cuts at: 1166 2120 1166 Size: 954 4684 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5638 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2264 2296 2264 Size: 32 5606 AlwNI CAG_nnn'CTG Cuts at: 2033 2424 3381 4953 2033 Size: 391 957 1572 2718 Fragments arranged by size: 2718 1572 957 391 ApaLI G'TGCA_C Cuts at: 3695 4851 3695 Size: 1156 4482 ApoI r'AATT_y Cuts at: 1493 2947 3173 3325 5434 1493 Size: 1454 226 152 2109 1697 Fragments arranged by size: 2109 1697 1454 226 152 AseI AT'TA_AT Cuts at: 160 1032 4192 160 Size: 872 3160 1606 Fragments arranged by size: 3160 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 AvaI C'yCGr_G Cuts at: 1870 1943 2963 1870 Size: 73 1020 4545 Fragments arranged by size: 4545 1020 73 BamHI G'GATC_C Cuts at: 2968 3335 2968 Size: 367 5271 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2959 4414 618 Size: 325 217 627 1172 1455 1842 Fragments arranged by size: 1842 1455 1172 627 325 217 BanII G_rGCy'C Cuts at: 1391 2957 1391 Size: 1566 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2817 928 Size: 971 918 3749 Fragments arranged by size: 3749 971 918 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3829 3863 1903 Size: 34 1892 34 3678 Fragments arranged by size: 3678 1892 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2687 3530 4740 2687 Size: 843 1210 3585 Fragments arranged by size: 3585 1210 843 BclI T'GATC_A Cuts at: 1548 1548 Size: 5638 BfrBI ATG'CAT Cuts at: 2295 2295 Size: 5638 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4249 136 Size: 107 122 71 631 605 2577 1525 Fragments arranged by size: 2577 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5633 5633 Size: 5638 BlpI GC'TnA_GC Cuts at: 3055 3055 Size: 5638 Bme1580I G_kGCm'C Cuts at: 1790 3699 4855 1790 Size: 1909 1156 2573 Fragments arranged by size: 2573 1909 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5638 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2465 3369 4327 Size: 416 53 83 399 24 1048 904 958 Cuts at: 4327 442 Size: 1753 Fragments arranged by size: 1753 1048 958 904 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5638 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2160 2557 4298 1239 Size: 921 397 1741 2579 Fragments arranged by size: 2579 1741 921 397 Bpu10I CC'TnA_GC Cuts at: 2626 2626 Size: 5638 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3763 4628 4926 3763 Size: 865 298 4475 Fragments arranged by size: 4475 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2520 4301 882 Size: 617 1021 1781 2219 Fragments arranged by size: 2219 1781 1021 617 BsaAI yAC'GTr Cuts at: 493 1204 3429 493 Size: 711 2225 2702 Fragments arranged by size: 2702 2225 711 BsaBI GATnn'nnATC Cuts at: 5632 5632 Size: 5638 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3445 3827 4462 275 Size: 53 83 186 936 1912 382 635 1451 Fragments arranged by size: 1912 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4071 4469 4743 4890 5301 4071 Size: 398 274 147 411 4408 Fragments arranged by size: 4408 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2073 2103 2264 2294 1178 Size: 30 165 30 670 30 161 30 4522 Fragments arranged by size: 4522 670 165 161 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2215 2282 2628 1186 Size: 1029 67 346 4196 Fragments arranged by size: 4196 1029 346 67 BseYI C'CCAG_C Cuts at: 4841 5337 4841 Size: 496 5142 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5065 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3088 3849 4877 1540 Size: 275 186 1087 761 1028 2301 Fragments arranged by size: 2301 1087 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3699 3784 4855 3699 Size: 85 1071 4482 Fragments arranged by size: 4482 1071 85 BsmI GAATG_Cn' Cuts at: 2297 3149 3242 2297 Size: 852 93 4693 Fragments arranged by size: 4693 852 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2957 3699 3784 4855 1391 Size: 399 1167 742 85 1071 2174 Fragments arranged by size: 2174 1167 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5301 5301 Size: 5638 BspHI T'CATG_A Cuts at: 3527 5257 3527 Size: 1730 3908 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2993 844 Size: 2149 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3525 1394 Size: 429 1702 3507 Fragments arranged by size: 3507 1702 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4133 4307 65 Size: 1183 2885 174 1396 Fragments arranged by size: 2885 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4282 4469 1700 Size: 110 2472 187 2869 Fragments arranged by size: 2869 2472 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5638 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5638 BssSI C'ACGA_G Cuts at: 3698 4710 3698 Size: 1012 4626 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5638 BstBI TT'CG_AA Cuts at: 4442 4442 Size: 5638 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5638 BstXI CCAn_nnnn'nTGG Cuts at: 2437 2437 Size: 5638 BstYI r'GATC_y Cuts at: 1972 2968 3335 3720 3737 5189 5275 5287 5633 Size: 996 367 385 17 1452 86 12 346 Cuts at: 5633 1972 Size: 1977 Fragments arranged by size: 1977 1452 996 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2449 2449 Size: 5638 Bsu36I CC'TnA_GG Cuts at: 2879 2879 Size: 5638 BtgI C'CryG_G Cuts at: 513 1065 2004 2355 513 Size: 552 939 351 3796 Fragments arranged by size: 3796 939 552 351 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4899 Fragments arranged by size: 4899 683 56 BtsI GCAGTG_nn' Cuts at: 2390 3154 3948 3968 2390 Size: 764 794 20 4060 Fragments arranged by size: 4060 794 764 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5638 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5603 DraI TTT'AAA Cuts at: 2942 2942 Size: 5638 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4645 809 Size: 507 3329 1802 Fragments arranged by size: 3329 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3085 3974 5603 Size: 54 1425 50 275 18 1255 889 1629 Cuts at: 5603 8 Size: 43 Fragments arranged by size: 1629 1425 1255 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3085 1537 Size: 275 1273 4090 Fragments arranged by size: 4090 1273 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2090 3568 1353 Size: 737 1478 3423 Fragments arranged by size: 3423 1478 737 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4225 4599 4745 387 Size: 1064 138 2636 374 146 1280 Fragments arranged by size: 2636 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2010 2160 2557 2927 3701 4298 5085 Size: 393 378 150 397 370 774 597 787 Cuts at: 5085 5353 1239 Size: 268 1524 Fragments arranged by size: 1524 787 774 597 397 393 378 370 268 150 EcoRI G'AATT_C Cuts at: 2947 3325 2947 Size: 378 5260 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2360 2360 Size: 5638 FspI TGC'GCA Cuts at: 4144 4144 Size: 5638 HaeII r_GCGC'y Cuts at: 1678 4785 1678 Size: 3107 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2690 2722 2807 2839 4279 Size: 32 135 32 1118 32 85 32 1440 Cuts at: 4279 4311 4353 4385 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1440 1118 135 85 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2982 3228 669 Size: 988 281 1044 246 3079 Fragments arranged by size: 3079 1044 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5638 HpaI GTT'AAC Cuts at: 3228 3228 Size: 5638 KpnI G_GTAC'C Cuts at: 2963 2963 Size: 5638 MfeI C'AATT_G Cuts at: 3237 3237 Size: 5638 MluI A'CGCG_T Cuts at: 5514 5514 Size: 5638 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4752 4936 1305 Size: 3447 184 2007 Fragments arranged by size: 3447 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3816 Fragments arranged by size: 3816 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2120 2435 2495 2586 2804 3596 3955 Size: 648 954 315 60 91 218 792 359 Cuts at: 3955 4114 518 Size: 159 2042 Fragments arranged by size: 2042 954 792 648 359 315 218 159 91 60 MspA1I CmG'CkG Cuts at: 1606 3054 3731 4879 5124 1606 Size: 1448 677 1148 245 2120 Fragments arranged by size: 2120 1448 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2355 513 Size: 552 939 351 3796 Fragments arranged by size: 3796 939 552 351 NdeI CA'TA_TG Cuts at: 387 387 Size: 5638 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5638 NotI GC'GGCC_GC Cuts at: 3085 3085 Size: 5638 NsiI A_TGCA'T Cuts at: 2297 2297 Size: 5638 NspI r_CATG'y Cuts at: 1132 1229 2494 2996 4541 1132 Size: 97 1265 502 1545 2229 Fragments arranged by size: 2229 1545 1265 502 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5638 PciI A'CATG_T Cuts at: 4537 4537 Size: 5638 PflMI CCAn_nnn'nTGG Cuts at: 1108 2740 1108 Size: 1632 4006 PmeI GTTT'AAAC Cuts at: 2942 2942 Size: 5638 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2264 2296 3707 3739 5220 5252 2264 Size: 32 1411 32 1481 32 2650 Fragments arranged by size: 2650 1481 1411 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5638 PsiI TTA'TAA Cuts at: 3208 3208 Size: 5638 PstI C_TGCA'G Cuts at: 830 2990 830 Size: 2160 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5638 SalI G'TCGA_C Cuts at: 1936 2980 1936 Size: 1044 4594 SbfI CC_TGCA'GG Cuts at: 2990 2990 Size: 5638 ScaI AGT'ACT Cuts at: 3886 5598 3886 Size: 1712 3926 SfcI C'TryA_G Cuts at: 826 1045 2509 2872 2986 4121 4802 4993 826 Size: 219 1464 363 114 1135 681 191 1471 Fragments arranged by size: 1471 1464 1135 681 363 219 191 114 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5638 SmaI CCC'GGG Cuts at: 1872 2965 1872 Size: 1093 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3742 4643 4905 820 Size: 197 926 1799 901 262 1553 Fragments arranged by size: 1799 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3429 493 Size: 2936 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5638 SphI G_CATG'C Cuts at: 1132 2996 1132 Size: 1864 3774 SspI AAT'ATT Cuts at: 5 52 2404 3562 5 Size: 47 2352 1158 2081 Fragments arranged by size: 2352 2081 1158 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5638 StyI C'CwwG_G Cuts at: 513 1065 2004 2098 2355 2680 3077 513 Size: 552 939 94 257 325 397 3074 Fragments arranged by size: 3074 939 552 397 325 257 94 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2594 3665 3682 3835 4020 4465 325 Size: 1255 1014 1071 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1071 1014 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3884 5596 96 Size: 274 80 33 51 1443 1907 1712 138 Fragments arranged by size: 1907 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2073 2094 2105 2126 2628 2660 5598 5630 2073 Size: 21 11 21 502 32 2938 32 2081 Fragments arranged by size: 2938 2081 502 32 32 21 21 11 Tth111I GACn'n_nGTC Cuts at: 1316 2563 1316 Size: 1247 4391 XbaI T'CTAG_A Cuts at: 2974 2974 Size: 5638 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2803 2803 Size: 5638 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5638 XmaI C'CCGG_G Cuts at: 1870 2963 1870 Size: 1093 4545 XmnI GAAnn'nnTTC Cuts at: 3767 3767 Size: 5638 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3446 276 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI