################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BanII BbeI BbrPI BbsI BcgI BciVI BcnI BfrI BglII BlnI BpuAI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BsrDI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cac8I CfoI Cfr10I ClaI CpoI DraI DraII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HapII HhaI HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MfeI MluI MroI MseI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 793 GACGTC GACGT^C AflIII 203 ACATGT A^C[GA][CT]GT AvaI 618 CTCGGG C^[CT]CG[GA]G BamHI 677 GGATCC G^GATCC BanI 225 GGTGCC G^G[CT][GA]CC BbvCI 572 CCTCAGC CCTCAGC(-5/-2) BclI 137 TGATCA T^GATCA BfaI 610 CTAG C^TAG BglI 421 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 292 GCTCAGC GC^T[ACGT]AGC BpmI 786 CTCCAG CTGGAG(16/14) Bpu1102I 292 GCTCAGC GC^T[ACGT]AGC BsaAI 97 TACGTG [CT]AC^GT[GA] BsaBI 585 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 777 GAGACC GGTCTC(1/5) BseRI 121 CTCCTC GAGGAG(10/8) BsoBI 618 CTCGGG C^[CT]CG[GA]G Bsp1407I 857 TGTACA T^GTACA BspLU11I 203 ACATGT A^CATGT BspMI 413 GCAGGT ACCTGC(4/8) BsrBI 382 CCGCTC CCGCTC(-3/-3) BsrGI 857 TGTACA T^GTACA BsrI 555 ACTGG ACTGG(1/-1) BssSI 16 CTCGTG CACGAG(-5/-1) BstXI 578 CCAACGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 292 GCTCAGC GC^T[ACGT]AGC DraIII 324 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 243 GACCTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 245 CCTTGG C^C[AT][AT]GG FauI 381 CCCGC CCCGC(4/6) FbaI 137 TGATCA T^GATCA HgaI 64 GACGC GACGC(5/10) MaeI 610 CTAG C^TAG MamI 585 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 282 CAGCTG C[AC]G^C[GT]G NspI 203 ACATGT [GA]CATG^[CT] PvuII 282 CAGCTG CAG^CTG SspBI 857 TGTACA T^GTACA StyI 245 CCTTGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcyI 64 GACGCC G[GA]^CG[CT]C 793 GACGTC AspHI 100 GTGCTC G[AT]GC[AT]^C 321 GAGCAC AvaII 505 GGACC G^G[AT]CC 738 GGTCC BbvI 284 GCTGC GCAGC(8/12) 623 GCTGC BsaHI 64 GACGCC G[GA]^CG[CT]C 793 GACGTC BsgI 457 CTGCAC GTGCAG(16/14) 563 GTGCAG BsiHKAI 100 GTGCTC G[AT]GC[AT]^C 321 GAGCAC BsmAI 777 GAGAC GTCTC(1/5) 905 GAGAC BsmFI 791 GGGAC GGGAC(10/14) 252 GTCCC BstYI 515 AGATCC [GA]^GATC[CT] 677 GGATCC Hin1I 64 GACGCC G[GA]^CG[CT]C 793 GACGTC HinfI 80 GAATC G^A[ACGT]TC 148 GATTC MaeII 98 ACGT A^CGT 794 ACGT MboII 882 GAAGA GAAGA(8/7) 83 TCTTC MflI 515 AGATCC [GA]^GATC[CT] 677 GGATCC SfaNI 535 GATGC GCATC(5/9) 731 GCATC TfiI 80 GAATC G^A[AT]TC 148 GATTC TseI 284 GCTGC G^C[AT]GC 623 GCTGC Tsp45I 19 GTGAC ^GT[GC]AC 220 GTCAC XcmI 545 CCATCCTGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 788 CCAGGGACGTCGTGG XhoII 515 AGATCC [GA]^GATC[CT] 677 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AfaI AluI AlwI BalI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HphI ItaI MaeIII MboI MluNI MnlI MscI MslI MvaI MwoI NdeII NlaIII NlaIV PflMI RsaI Sau3AI Sau96I ScrFI TspRI Van91I