################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BanII BbeI BbrPI BbsI BcgI BciVI BcnI BfaI BfrI BglII BlnI BpmI BpuAI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraII EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MfeI MluI MroI MseI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 700 CGCG CG^CG AflIII 203 ACATGT A^C[GA][CT]GT AocI 617 CCTCAGG CC^T[ACGT]AGG BamHI 677 GGATCC G^GATCC BanI 225 GGTGCC G^G[CT][GA]CC BbvCI 572 CCTCAGC CCTCAGC(-5/-2) BclI 137 TGATCA T^GATCA BglI 421 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 292 GCTCAGC GC^T[ACGT]AGC Bpu1102I 292 GCTCAGC GC^T[ACGT]AGC BsaAI 97 TACGTG [CT]AC^GT[GA] BsaBI 585 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmAI 905 GAGAC GTCTC(1/5) BsmFI 252 GTCCC GGGAC(10/14) Bsp1407I 857 TGTACA T^GTACA BspLU11I 203 ACATGT A^CATGT BspMI 413 GCAGGT ACCTGC(4/8) BsrBI 382 CCGCTC CCGCTC(-3/-3) BsrFI 433 ACCGGC [GA]^CCGG[CT] BsrGI 857 TGTACA T^GTACA BsrI 555 ACTGG ACTGG(1/-1) BssSI 16 CTCGTG CACGAG(-5/-1) BstUI 700 CGCG CG^CG BstXI 578 CCAACGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 617 CCTCAGG CC^T[ACGT]AGG Cac8I 10 GCCAGC GC[ACGT]^[ACGT]GC CelII 292 GCTCAGC GC^T[ACGT]AGC CfoI 699 GCGC GCG^C Cfr10I 433 ACCGGC [GA]^CCGG[CT] DraIII 324 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 243 GACCTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 617 CCTCAGG CC^T[ACGT]AGG FauI 381 CCCGC CCCGC(4/6) FbaI 137 TGATCA T^GATCA HapII 434 CCGG C^CGG HhaI 699 GCGC GCG^C HinP1I 699 GCGC G^CGC HpaII 434 CCGG C^CGG MaeII 98 ACGT A^CGT MamI 585 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 83 TCTTC GAAGA(8/7) MspA1I 282 CAGCTG C[AC]G^C[GT]G MspI 434 CCGG C^CGG MvnI 700 CGCG CG^CG NspI 203 ACATGT [GA]CATG^[CT] PflMI 309 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 282 CAGCTG CAG^CTG SfaNI 793 GATGC GCATC(5/9) SspBI 857 TGTACA T^GTACA StuI 39 AGGCCT AGG^CCT Van91I 309 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcyI 64 GACGCC G[GA]^CG[CT]C 834 GACGCC AspHI 100 GTGCTC G[AT]GC[AT]^C 321 GAGCAC AvaII 505 GGACC G^G[AT]CC 738 GGTCC BbvI 284 GCTGC GCAGC(8/12) 623 GCTGC BsaHI 64 GACGCC G[GA]^CG[CT]C 834 GACGCC BseRI 70 CTCCTC GAGGAG(10/8) 121 CTCCTC BsiHKAI 100 GTGCTC G[AT]GC[AT]^C 321 GAGCAC BstYI 515 AGATCC [GA]^GATC[CT] 677 GGATCC Cfr13I 505 GGACC G^G[ACGT]CC 738 GGTCC DsaI 788 CCATGG C^C[GA][CT]GG 797 CCATGG Hin1I 64 GACGCC G[GA]^CG[CT]C 834 GACGCC HinfI 80 GAATC G^A[ACGT]TC 148 GATTC MflI 515 AGATCC [GA]^GATC[CT] 677 GGATCC NcoI 788 CCATGG C^CATGG 797 CCATGG Sau96I 505 GGACC G^G[ACGT]CC 738 GGTCC TfiI 80 GAATC G^A[AT]TC 148 GATTC TseI 284 GCTGC G^C[AT]GC 623 GCTGC XcmI 545 CCATCCTGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 788 CCATGGATGCCATGG XhoII 515 AGATCC [GA]^GATC[CT] 677 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AfaI AluI AlwI BalI BmyI BsaJI BsgI BsiYI BslI Bsp1286I BssKI BstF5I BstNI DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HgaI HphI ItaI MaeIII MboI MluNI MnlI MscI MslI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI StyI Tsp45I TspRI