################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII BamHI BanI BbeI BbrPI BbsI BcgI BciVI BcnI BfrI BglI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MamI MluI MroI MspI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 711 GTCTAC GT^[AC][GT]AC Alw44I 422 GTGCAC G^TGCAC AlwI 646 GGATC GGATC(4/5) AlwNI 382 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 422 GTGCAC G^TGCAC AvrII 235 CCTAGG C^CTAGG BalI 887 TGGCCA TGG^CCA BanII 46 GGGCTC G[GA]GC[CT]^C BbvCI 544 CCTCAGC CCTCAGC(-5/-2) BglII 332 AGATCT A^GATCT BlnI 235 CCTAGG C^CTAGG BpmI 934 CTGGAG CTGGAG(16/14) BseRI 906 GAGGAG GAGGAG(10/8) BsmAI 811 GTCTC GTCTC(1/5) BsmBI 810 CGTCTC CGTCTC(1/5) BsmFI 929 GTCCC GGGAC(10/14) BspMI 161 ACCTGC ACCTGC(4/8) BsrI 42 ACTGG ACTGG(1/-1) BstXI 312 CCACTTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 332 AGATCT [GA]^GATC[CT] Cac8I 562 GCCTGC GC[ACGT]^[ACGT]GC DrdI 802 GACAAGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 797 CCATGG C^C[GA][CT]GG EaeI 887 TGGCCA [CT]^GGCC[GA] HincII 247 GTTGAC GT[CT]^[GA]AC HindII 247 GTTGAC GT[CT]^[GA]AC MaeII 767 ACGT A^CGT MfeI 827 CAATTG C^AATTG MflI 332 AGATCT [GA]^GATC[CT] MluNI 887 TGGCCA TGG^CCA MscI 887 TGGCCA TGG^CCA MunI 827 CAATTG C^AATTG NcoI 797 CCATGG C^CATGG PflMI 499 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 294 GACTC GAGTC(4/5) PpuMI 917 AGGACCC [GA]G^G[AT]CC[CT] SfcI 113 CTGTAG C^T[GA][CT]AG StuI 778 AGGCCT AGG^CCT Van91I 499 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 332 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) 696 CCGC AfaI 472 GTAC GT^AC 651 GTAC AspHI 422 GTGCAC G[AT]GC[AT]^C 937 GAGCAC AvaII 918 GGACC G^G[AT]CC 928 GGTCC BclI 551 TGATCA T^GATCA 881 TGATCA BfaI 236 CTAG C^TAG 664 CTAG BsiHKAI 422 GTGCAC G[AT]GC[AT]^C 937 GAGCAC BsrDI 369 CATTGC GCAATG(2/0) 729 CATTGC BstEII 615 GGTGACC G^GT[ACGT]ACC 738 GGTGACC BstPI 615 GGTGACC G^GT[ACGT]ACC 738 GGTGACC DraII 704 AGGCCCT [GA]G^G[ACGT]CC[CT] 917 AGGACCC EarI 219 CTCTTC CTCTTC(1/4) 301 CTCTTC EcoO109I 704 AGGCCCT [GA]G^G[ACGT]CC[CT] 917 AGGACCC EcoO65I 615 GGTGACC G^GT[ACGT]ACC 738 GGTGACC EcoT14I 235 CCTAGG C^C[AT][AT]GG 797 CCATGG FbaI 551 TGATCA T^GATCA 881 TGATCA Ksp632I 219 CTCTTC CTCTTC(1/4) 301 CTCTTC MaeI 236 CTAG C^TAG 664 CTAG MseI 592 TTAA T^TAA 745 TTAA MspA1I 493 CAGCTG C[AC]G^C[GT]G 547 CAGCTG NlaIV 918 GGACCC GG[ACGT]^[ACGT]CC 928 GGTCCC PvuII 493 CAGCTG CAG^CTG 547 CAGCTG RsaI 472 GTAC GT^AC 651 GTAC StyI 235 CCTAGG C^C[AT][AT]GG 797 CCATGG Tru9I 592 TTAA T^TAA 745 TTAA Tsp509I 557 AATT ^AATT 828 AATT TspRI 84 CACTG CA[GC]TG[ACGT][ACGT]^ 659 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AluI BbvI BmyI BsaJI BsgI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII Sau3AI Sau96I ScrFI SfaNI TfiI TseI Tsp45I