Restriction map for
pFN21AE5956
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BglI BglII BlnI BpmI BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraI
DraII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HhaI HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeII MamI MfeI MflI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 589 GGCGTC G[GA]^CG[CT]C
AflII 894 CTTAAG C^TTAAG
AlwNI 683 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 538 GGGCCC GGGCC^C
AseI 802 ATTAAT AT^TAAT
AsnI 802 ATTAAT AT^TAAT
Asp700I 674 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 432 GAGCTC G[AT]GC[AT]^C
BalI 690 TGGCCA TGG^CCA
BanI 316 GGCACC G^G[CT][GA]CC
BfrI 894 CTTAAG C^TTAAG
BlpI 145 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 145 GCTGAGC GC^T[ACGT]AGC
BsaHI 589 GGCGTC G[GA]^CG[CT]C
BseRI 789 CTCCTC GAGGAG(10/8)
BsiHKAI 432 GAGCTC G[AT]GC[AT]^C
BsmAI 736 GTCTC GTCTC(1/5)
BspHI 398 TCATGA T^CATGA
BstXI 569 CCAGCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 145 GCTGAGC GC^T[ACGT]AGC
DraIII 135 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 690 TGGCCA [CT]^GGCC[GA]
EcoNI 285 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 54 CCAAGG C^C[AT][AT]GG
HgaI 590 GCGTC GACGC(5/10)
Hin1I 589 GGCGTC G[GA]^CG[CT]C
HinfI 849 GAATC G^A[ACGT]TC
MluNI 690 TGGCCA TGG^CCA
MscI 690 TGGCCA TGG^CCA
MspA1I 421 CAGCTG C[AC]G^C[GT]G
NspI 272 GCATGT [GA]CATG^[CT]
PshAI 729 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 802 ATTAAT AT^TAAT
PvuII 421 CAGCTG CAG^CTG
RcaI 398 TCATGA T^CATGA
SacI 432 GAGCTC GAGCT^C
SfaNI 474 GATGC GCATC(5/9)
SmlI 894 CTTAAG C^T[CT][GA]AG
StyI 54 CCAAGG C^C[AT][AT]GG
TfiI 849 GAATC G^A[AT]TC
Tsp45I 621 GTCAC ^GT[GC]AC
XmnI 674 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AluI 422 AGCT AG^CT
433 AGCT
AlwI 333 GATCC GGATC(4/5)
612 GATCC
BfaI 37 CTAG C^TAG
238 CTAG
BsrDI 116 GCAATG GCAATG(2/0)
904 GCAATG
BstAPI 272 GCATGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
407 GCAAACTCTGC
DpnII 333 GATC ^GATC
612 GATC
EcoRI 25 GAATTC G^AATTC
674 GAATTC
MaeI 37 CTAG C^TAG
238 CTAG
MaeIII 185 GTAAC ^GT[ACGT]AC
621 GTCAC
MboI 333 GATC ^GATC
612 GATC
MwoI 272 GCATGTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
407 GCAAACTCTGC
NdeII 333 GATC ^GATC
612 GATC
PstI 289 CTGCAG CTGCA^G
418 CTGCAG
Sau3AI 333 GATC ^GATC
612 GATC
SfcI 289 CTGCAG C^T[GA][CT]AG
418 CTGCAG
StuI 57 AGGCCT AGG^CCT
293 AGGCCT
TspRI 135 CACTG CA[GC]TG[ACGT][ACGT]^
687 CACTG
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AcsI AfaI ApoI BanII
BbvI BmyI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EcoRII Fnu4HI
FokI HaeIII HphI ItaI MboII
MnlI MseI MslI MvaI NlaIII
NlaIV PflMI RsaI Sau96I ScrFI
Tru9I TseI Tsp509I Van91I