Restriction map for
pFN21AE6057
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BcgI BciVI BcnI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MspA1I MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tru9I Tth111I TthHB8I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AflIII 337 ACATGT A^C[GA][CT]GT
AlwI 147 GATCC GGATC(4/5)
AlwNI 453 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
BanI 760 GGCACC G^G[CT][GA]CC
BanII 169 GAGCCC G[GA]GC[CT]^C
BbsI 43 GTCTTC GAAGAC(2/6)
BbvCI 288 CCTCAGC CCTCAGC(-5/-2)
BfaI 706 CTAG C^TAG
BpuAI 43 GTCTTC GAAGAC(2/6)
BsaAI 790 TACGTG [CT]AC^GT[GA]
BsaI 76 GAGACC GGTCTC(1/5)
BsgI 403 CTGCAC GTGCAG(16/14)
BspHI 419 TCATGA T^CATGA
BspLU11I 337 ACATGT A^CATGT
BsrI 452 CCAGT ACTGG(1/-1)
BstAPI 448 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 499 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 155 TGGCCG [CT]^GGCC[GA]
EcoO109I 499 AGGACCT [GA]G^G[ACGT]CC[CT]
HinfI 22 GAATC G^A[ACGT]TC
MaeI 706 CTAG C^TAG
MaeII 791 ACGT A^CGT
MaeIII 947 GTGAC ^GT[ACGT]AC
NcoI 623 CCATGG C^CATGG
NspI 337 ACATGT [GA]CATG^[CT]
PpuMI 499 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 419 TCATGA T^CATGA
SmlI 932 CTCAAG C^T[CT][GA]AG
StuI 572 AGGCCT AGG^CCT
TfiI 22 GAATC G^A[AT]TC
Tsp45I 947 GTGAC ^GT[GC]AC
Tsp509I 864 AATT ^AATT
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# TWO CUT ENZYMES ( 19 enzymes) #
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AciI 282 CCGC CCGC(-3/-1)
378 CCGC
AfaI 192 GTAC GT^AC
318 GTAC
AluI 292 AGCT AG^CT
739 AGCT
BclI 203 TGATCA T^GATCA
552 TGATCA
BmyI 169 GAGCCC G[AGT]GC[ACT]^C
759 GGGCAC
BseRI 109 CTCCTC GAGGAG(10/8)
472 CTCCTC
BsmAI 76 GAGAC GTCTC(1/5)
930 GTCTC
BsmFI 278 GGGAC GGGAC(10/14)
247 GTCCC
Bsp1286I 169 GAGCCC G[AGT]GC[ACT]^C
759 GGGCAC
BspMI 733 ACCTGC ACCTGC(4/8)
809 ACCTGC
BsrDI 525 CATTGC GCAATG(2/0)
836 GCAATG
DsaI 158 CCGTGG C^C[GA][CT]GG
623 CCATGG
EarI 112 CTCTTC CTCTTC(1/4)
322 CTCTTC
FbaI 203 TGATCA T^GATCA
552 TGATCA
Ksp632I 112 CTCTTC CTCTTC(1/4)
322 CTCTTC
NlaIV 278 GGGACC GG[ACGT]^[ACGT]CC
760 GGCACC
PstI 310 CTGCAG CTGCA^G
735 CTGCAG
RsaI 192 GTAC GT^AC
318 GTAC
XcmI 434 CCAATGCTACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
782 CCATAGCCTACGTGG
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AvaII BbvI BglI BsaJI BsiYI
BslI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EcoRII EcoT14I Fnu4HI
FokI HaeIII HphI ItaI MboI
MboII MnlI MslI MvaI MwoI
NdeII NlaIII PflMI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
TspRI Van91I