(Linear) (Six-base) MAPSORT of: KIEE1931.seq Check: 4962 from: 1 to: 1335 KIEE1931.orf With 182 enzymes: SgfI * July 15, 2010 18:51 .. AatII G_ACGT'C Cuts at: 0 248 1335 Size: 248 1087 AccI GT'mk_AC Cuts at: 0 316 352 1001 1335 Size: 316 36 649 334 Fragments arranged by size: 649 334 316 36 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 41 892 1335 Size: 41 851 443 Fragments arranged by size: 851 443 41 AflIII A'CryG_T Cuts at: 0 374 428 1335 Size: 374 54 907 Fragments arranged by size: 907 374 54 AleI CACnn'nnGTG Cuts at: 0 831 1335 Size: 831 504 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 128 160 1335 Size: 128 32 1175 Fragments arranged by size: 1175 128 32 ApaI G_GGCC'C Cuts at: 0 460 1063 1221 1327 1335 Size: 460 603 158 106 8 Fragments arranged by size: 603 460 158 106 8 AvaI C'yCGr_G Cuts at: 0 684 1335 Size: 684 651 BanI G'GyrC_C Cuts at: 0 1240 1335 Size: 1240 95 BanII G_rGCy'C Cuts at: 0 73 122 460 864 1063 1221 1327 1335 Size: 73 49 338 404 199 158 106 8 Fragments arranged by size: 404 338 199 158 106 73 49 8 BbsI GAAGACnn'nnnn_ Cuts at: 0 1207 1335 Size: 1207 128 BbvCI CC'TCA_GC Cuts at: 0 934 1335 Size: 934 401 BglI GCCn_nnn'nGGC Cuts at: 0 929 1116 1335 Size: 929 187 219 Fragments arranged by size: 929 219 187 BglII A'GATC_T Cuts at: 0 701 1335 Size: 701 634 BlpI GC'TnA_GC Cuts at: 0 744 1335 Size: 744 591 Bme1580I G_kGCm'C Cuts at: 0 460 925 1019 1063 1221 1327 1335 Size: 460 465 94 44 158 106 8 Fragments arranged by size: 465 460 158 106 94 44 8 BmgBI CAC'GTC Cuts at: 0 431 1020 1188 1335 Size: 431 589 168 147 Fragments arranged by size: 589 431 168 147 BmrI ACTGGGnnnn_n' Cuts at: 0 912 1224 1335 Size: 912 312 111 Fragments arranged by size: 912 312 111 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 458 901 1335 Size: 34 424 443 434 Fragments arranged by size: 443 434 424 34 Bpu10I CC'TnA_GC Cuts at: 0 934 1335 Size: 934 401 BsaHI Gr'CG_yC Cuts at: 0 245 1335 Size: 245 1090 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 244 274 629 659 1335 Size: 244 30 355 30 676 Fragments arranged by size: 676 355 244 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 75 130 455 1335 Size: 75 55 325 880 Fragments arranged by size: 880 325 75 55 BseYI C'CCAG_C Cuts at: 0 749 887 952 957 1037 1335 Size: 749 138 65 5 80 298 Fragments arranged by size: 749 298 138 80 65 5 BsiEI CG_ry'CG Cuts at: 0 220 1335 Size: 220 1115 BsiHKAI G_wGCw'C Cuts at: 0 475 1335 Size: 475 860 BsmBI CGTCTCn'nnnn_ Cuts at: 0 279 1335 Size: 279 1056 Bsp1286I G_dGCh'C Cuts at: 0 73 122 460 475 864 925 1019 1063 Size: 73 49 338 15 389 61 94 44 Cuts at: 1063 1221 1327 1335 Size: 158 106 8 Fragments arranged by size: 389 338 158 106 94 73 61 49 44 15 8 BsrBI CCG'CTC Cuts at: 0 270 392 586 743 1056 1335 Size: 270 122 194 157 313 279 Fragments arranged by size: 313 279 270 194 157 122 BsrDI GCAATG_nn' Cuts at: 0 727 1335 Size: 727 608 BsrFI r'CCGG_y Cuts at: 0 188 215 512 843 1046 1132 1288 1335 Size: 188 27 297 331 203 86 156 47 Fragments arranged by size: 331 297 203 188 156 86 47 27 BsrGI T'GTAC_A Cuts at: 0 722 1335 Size: 722 613 BstYI r'GATC_y Cuts at: 0 701 1335 Size: 701 634 BstZ17I GTA'TAC Cuts at: 0 317 353 1335 Size: 317 36 982 Fragments arranged by size: 982 317 36 BtgI C'CryG_G Cuts at: 0 294 926 963 1023 1032 1236 1335 Size: 294 632 37 60 9 204 99 Fragments arranged by size: 632 294 204 99 60 37 9 BtsI GCAGTG_nn' Cuts at: 0 826 1228 1335 Size: 826 402 107 Fragments arranged by size: 826 402 107 EaeI y'GGCC_r Cuts at: 0 387 514 587 966 1122 1182 1335 Size: 387 127 73 379 156 60 153 Fragments arranged by size: 387 379 156 153 127 73 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 612 1297 1335 Size: 612 685 38 Fragments arranged by size: 685 612 38 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 41 458 892 901 1335 Size: 34 7 417 434 9 434 Fragments arranged by size: 434 434 417 34 9 7 EcoNI CCTnn'n_nnAGG Cuts at: 0 440 1335 Size: 440 895 EcoO109I rG'GnC_Cy Cuts at: 0 29 456 457 1059 1060 1099 1277 1323 Size: 29 427 1 602 1 39 178 46 Cuts at: 1323 1335 Size: 12 Fragments arranged by size: 602 427 178 46 39 29 12 1 1 FseI GG_CCGG'CC Cuts at: 0 1136 1335 Size: 1136 199 FspI TGC'GCA Cuts at: 0 104 1335 Size: 104 1231 HaeII r_GCGC'y Cuts at: 0 979 1335 Size: 979 356 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 727 759 1335 Size: 727 32 576 Fragments arranged by size: 727 576 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 806 1335 Size: 806 529 MscI TGG'CCA Cuts at: 0 1124 1335 Size: 1124 211 MslI CAynn'nnrTG Cuts at: 0 373 831 1022 1335 Size: 373 458 191 313 Fragments arranged by size: 458 373 313 191 MspA1I CmG'CkG Cuts at: 0 544 756 891 1010 1329 1335 Size: 544 212 135 119 319 6 Fragments arranged by size: 544 319 212 135 119 6 NaeI GCC'GGC Cuts at: 0 190 514 1048 1134 1335 Size: 190 324 534 86 201 Fragments arranged by size: 534 324 201 190 86 NcoI C'CATG_G Cuts at: 0 1023 1335 Size: 1023 312 NgoMIV G'CCGG_C Cuts at: 0 188 512 1046 1132 1335 Size: 188 324 534 86 203 Fragments arranged by size: 534 324 203 188 86 NspI r_CATG'y Cuts at: 0 378 1335 Size: 378 957 PasI CC'CwG_GG Cuts at: 0 995 1150 1335 Size: 995 155 185 Fragments arranged by size: 995 185 155 PciI A'CATG_T Cuts at: 0 374 1335 Size: 374 961 PflMI CCAn_nnn'nTGG Cuts at: 0 964 1335 Size: 964 371 PfoI T'CCnGG_A Cuts at: 0 325 1335 Size: 325 1010 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 55 87 1335 Size: 55 32 1248 Fragments arranged by size: 1248 55 32 PpuMI rG'GwC_Cy Cuts at: 0 29 1335 Size: 29 1306 PspOMI G'GGCC_C Cuts at: 0 456 1059 1217 1323 1335 Size: 456 603 158 106 12 Fragments arranged by size: 603 456 158 106 12 PstI C_TGCA'G Cuts at: 0 794 1335 Size: 794 541 SanDI GG'GwC_CC Cuts at: 0 29 1335 Size: 29 1306 SfcI C'TryA_G Cuts at: 0 790 1335 Size: 790 545 SgrAI Cr'CCGG_yG Cuts at: 0 188 843 1335 Size: 188 655 492 Fragments arranged by size: 655 492 188 SmaI CCC'GGG Cuts at: 0 686 1335 Size: 686 649 StyI C'CwwG_G Cuts at: 0 278 573 1023 1335 Size: 278 295 450 312 Fragments arranged by size: 450 312 295 278 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 327 696 1258 1335 Size: 327 369 562 77 Fragments arranged by size: 562 369 327 77 TatI w'GTAC_w Cuts at: 0 722 1335 Size: 722 613 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 244 276 629 661 1335 Size: 244 32 353 32 674 Fragments arranged by size: 674 353 244 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 639 1335 Size: 639 696 XmaI C'CCGG_G Cuts at: 0 684 1335 Size: 684 651 ZraI GAC'GTC Cuts at: 0 246 1335 Size: 246 1089 Enzymes that do cut: AatII AccI AcuI AflIII AleI AloI ApaI AvaI BanI BanII BbsI BbvCI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BsrFI BsrGI BstYI BstZ17I BtgI BtsI EaeI EciI Eco57MI EcoNI EcoO109I FseI FspI HaeII Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PciI PflMI PfoI PpiI PpuMI PspOMI PstI SanDI SfcI SgrAI SmaI StyI TaqII TatI TstI XcmI XmaI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsgI BsiWI BsmI BspEI BspHI BspMI BssHII BssSI BstAPI BstBI BstEII BstXI Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoRI EcoRV FalI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmnI