################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbvCI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcsI 603 GAATTT [GA]^AATT[CT] ApoI 603 GAATTT [GA]^AATT[CT] AspHI 471 GAGCTC G[AT]GC[AT]^C BanII 471 GAGCTC G[GA]GC[CT]^C BbrPI 726 CACGTG CAC^GTG BbsI 276 GAAGAC GAAGAC(2/6) BcgI 369 CGACCGATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 152 TGATCA T^GATCA BfaI 811 CTAG C^TAG BmyI 471 GAGCTC G[AGT]GC[ACT]^C BpuAI 276 GAAGAC GAAGAC(2/6) BsaAI 726 CACGTG [CT]AC^GT[GA] BsgI 172 CTGCAC GTGCAG(16/14) BsiEI 369 CGACCG CG[GA][CT]^CG BsiHKAI 471 GAGCTC G[AT]GC[AT]^C Bsp1286I 471 GAGCTC G[AGT]GC[ACT]^C Bsp1407I 430 TGTACA T^GTACA BspHI 413 TCATGA T^CATGA BsrGI 430 TGTACA T^GTACA BsrI 556 CCAGT ACTGG(1/-1) BssSI 610 CTCGTG CACGAG(-5/-1) BstEII 618 GGTCACC G^GT[ACGT]ACC BstPI 618 GGTCACC G^GT[ACGT]ACC BstYI 689 AGATCC [GA]^GATC[CT] EarI 228 CTCTTC CTCTTC(1/4) EcoO65I 618 GGTCACC G^GT[ACGT]ACC FbaI 152 TGATCA T^GATCA Ksp632I 228 CTCTTC CTCTTC(1/4) MaeI 811 CTAG C^TAG MaeII 727 ACGT A^CGT MflI 689 AGATCC [GA]^GATC[CT] MwoI 464 GCTTCCTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 452 CATATG CA^TATG PflMI 11 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 753 GACTC GAGTC(4/5) PmaCI 726 CACGTG CAC^GTG PmlI 726 CACGTG CAC^GTG RcaI 413 TCATGA T^CATGA SacI 471 GAGCTC GAGCT^C SspBI 430 TGTACA T^GTACA TfiI 56 GATTC G^A[AT]TC Tsp509I 604 AATT ^AATT Van91I 11 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 281 CCATATCATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 689 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AciI 515 GCGG CCGC(-3/-1) 679 GCGG AflIII 725 ACACGT A^C[GA][CT]GT 727 ACGTGT AvaII 517 GGACC G^G[AT]CC 681 GGTCC BbvI 379 GCAGC GCAGC(8/12) 301 GCTGC BseRI 85 CTCCTC GAGGAG(10/8) 346 CTCCTC BsmFI 766 GGGAC GGGAC(10/14) 818 GGGAC Cac8I 487 GCCAGC GC[ACGT]^[ACGT]GC 718 GCCTGC DraII 80 AGGCCCT [GA]G^G[ACGT]CC[CT] 422 AGGGCCT EcoNI 477 CCTGATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 909 CCTGTGGAAGG EcoO109I 80 AGGCCCT [GA]G^G[ACGT]CC[CT] 422 AGGGCCT Fnu4HI 301 GCTGC GC^[ACGT]GC 379 GCAGC HinfI 56 GATTC G^A[ACGT]TC 753 GACTC ItaI 301 GCTGC GC^[ACGT]GC 379 GCAGC MslI 177 CACACCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 408 CACTATCATG TseI 301 GCTGC G^C[AT]GC 379 GCAGC TspRI 32 CAGTG CA[GC]TG[ACGT][ACGT]^ 255 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AfaI AluI AlwI BpmI BsaJI BsiYI BslI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII FokI HaeIII HphI MaeIII MboI MboII MnlI MvaI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI Tsp45I