(Linear) (Six-base) MAPSORT of: KIEE6016.seq Check: 9656 from: 1 to: 945 KIEE6016.orf With 182 enzymes: SgfI * December 5, 2014 13:19 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 680 706 945 Size: 680 26 239 Fragments arranged by size: 680 239 26 AflIII A'CryG_T Cuts at: 0 725 727 945 Size: 725 2 218 Fragments arranged by size: 725 218 2 ApoI r'AATT_y Cuts at: 0 603 945 Size: 603 342 BanII G_rGCy'C Cuts at: 0 475 945 Size: 475 470 BbsI GAAGACnn'nnnn_ Cuts at: 0 283 945 Size: 283 662 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 358 392 945 Size: 358 34 553 Fragments arranged by size: 553 358 34 BclI T'GATC_A Cuts at: 0 152 945 Size: 152 793 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 61 372 783 945 Size: 61 311 411 162 Fragments arranged by size: 411 311 162 61 Bpu10I CC'TnA_GC Cuts at: 0 196 469 945 Size: 196 273 476 Fragments arranged by size: 476 273 196 BsaAI yAC'GTr Cuts at: 0 728 945 Size: 728 217 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 76 337 945 Size: 76 261 608 Fragments arranged by size: 608 261 76 BseYI C'CCAG_C Cuts at: 0 793 945 Size: 793 152 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 157 945 Size: 157 788 BsiEI CG_ry'CG Cuts at: 0 372 945 Size: 372 573 BsiHKAI G_wGCw'C Cuts at: 0 475 945 Size: 475 470 Bsp1286I G_dGCh'C Cuts at: 0 475 945 Size: 475 470 BspHI T'CATG_A Cuts at: 0 413 945 Size: 413 532 BsrGI T'GTAC_A Cuts at: 0 430 945 Size: 430 515 BssSI C'ACGA_G Cuts at: 0 610 945 Size: 610 335 BstEII G'GTnAC_C Cuts at: 0 618 945 Size: 618 327 BstYI r'GATC_y Cuts at: 0 689 945 Size: 689 256 EarI CTCTTCn'nnn_ Cuts at: 0 234 945 Size: 234 711 EcoICRI GAG'CTC Cuts at: 0 473 945 Size: 473 472 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 61 372 680 706 783 945 Size: 61 311 308 26 77 162 Fragments arranged by size: 311 308 162 77 61 26 EcoNI CCTnn'n_nnAGG Cuts at: 0 481 913 945 Size: 481 432 32 Fragments arranged by size: 481 432 32 EcoO109I rG'GnC_Cy Cuts at: 0 81 423 945 Size: 81 342 522 Fragments arranged by size: 522 342 81 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 509 541 945 Size: 509 32 404 Fragments arranged by size: 509 404 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 606 669 902 945 Size: 606 63 233 43 Fragments arranged by size: 606 233 63 43 MslI CAynn'nnrTG Cuts at: 0 181 412 945 Size: 181 231 533 Fragments arranged by size: 533 231 181 NdeI CA'TA_TG Cuts at: 0 453 945 Size: 453 492 PasI CC'CwG_GG Cuts at: 0 420 945 Size: 420 525 PflMI CCAn_nnn'nTGG Cuts at: 0 17 945 Size: 17 928 PmlI CAC'GTG Cuts at: 0 728 945 Size: 728 217 SacI G_AGCT'C Cuts at: 0 475 945 Size: 475 470 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 181 195 386 945 Size: 181 14 191 559 Fragments arranged by size: 559 191 181 14 TatI w'GTAC_w Cuts at: 0 185 430 782 945 Size: 185 245 352 163 Fragments arranged by size: 352 245 185 163 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 51 83 895 927 945 Size: 51 32 812 32 18 Fragments arranged by size: 812 51 32 32 18 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 288 945 Size: 288 657 Enzymes that do cut: AcuI AflIII ApoI BanII BbsI BcgI BclI BpmI Bpu10I BsaAI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrGI BssSI BstEII BstYI EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I MmeI MslI NdeI PasI PflMI PmlI SacI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI